Genetic diversity of wheat (Triticum aestivum L.) plants-regenerants produced by anther culture
The study of genetic diversity of wheat (Triticum aestivum L.) plants-regenerants produced by anther culture method from hybrids involved in the Latvian wheat breeding programme was performed. Flow cytometry was used to test ploidy of 3×103 cells of each green plantregenerant, and universal retrotra...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2016-09-01
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| Series: | Вавиловский журнал генетики и селекции |
| Subjects: | |
| Online Access: | https://vavilov.elpub.ru/jour/article/view/703 |
| _version_ | 1797214086311706624 |
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| author | D. Grauda K. Žagata G. Lanka V. Strazdina V. Fetere N. Lisina N. Krasnevska O. Fokina A. Mikelsone R. Ornicans I. Belogrudova I. Rashal |
| author_facet | D. Grauda K. Žagata G. Lanka V. Strazdina V. Fetere N. Lisina N. Krasnevska O. Fokina A. Mikelsone R. Ornicans I. Belogrudova I. Rashal |
| author_sort | D. Grauda |
| collection | DOAJ |
| description | The study of genetic diversity of wheat (Triticum aestivum L.) plants-regenerants produced by anther culture method from hybrids involved in the Latvian wheat breeding programme was performed. Flow cytometry was used to test ploidy of 3×103 cells of each green plantregenerant, and universal retrotransposon based iPBS (inter primer binding sites) method were used to establish genetic diversity of plants-regenerants. Progenies of 13 genetically distant hybrids were involved in the study. Most of plants-regenerants have leaves with mixoploid cells. Seeds were formed only by plants-regenerants that had cells with (2n = 6x) ploidy. Majority of fertile plantsregenerants have more than 40 % of hexaploid (6x) cells. The percentage of 6x cells in plants-regenerants and diversity in cell ploidy demonstrated association with mother plant (hybrid) genotype. Percent of spontaneous diplodization was also genotype-dependent. In this experiment colchicine treatment had no significant influence on outcome of fertile plants- regenerants. New dominant allele in a hybrid plant was found in comparison with both parents what indicated possible retrotransposon moving. Genetic diversity of the plants-regenerants obtained in anther culture is a combination of parent’s allele segregation and somaclonal variation. |
| first_indexed | 2024-03-07T16:07:11Z |
| format | Article |
| id | doaj.art-7c7b76593c4b4397a4f4e7ee12e12eb0 |
| institution | Directory Open Access Journal |
| issn | 2500-3259 |
| language | English |
| last_indexed | 2024-04-24T11:08:35Z |
| publishDate | 2016-09-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj.art-7c7b76593c4b4397a4f4e7ee12e12eb02024-04-11T15:30:57ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592016-09-0120453754410.18699/VJ16.176505Genetic diversity of wheat (Triticum aestivum L.) plants-regenerants produced by anther cultureD. Grauda0K. Žagata1G. Lanka2V. Strazdina3V. Fetere4N. Lisina5N. Krasnevska6O. Fokina7A. Mikelsone8R. Ornicans9I. Belogrudova10I. Rashal11Institute of Biology, University of LatviaInstitute of Biology, University of LatviaInstitute of Biology, University of LatviaStende Research Centre of the Institute of Agricultural Resources and Economics, Latvian University of AgricultureStende Research Centre of the Institute of Agricultural Resources and Economics, Latvian University of AgricultureInstitute of Biology, University of LatviaInstitute of Biology, University of LatviaInstitute of Biology, University of LatviaInstitute of Biology, University of LatviaInstitute of Biology, University of LatviaInstitute of Biology, University of LatviaInstitute of Biology, University of LatviaThe study of genetic diversity of wheat (Triticum aestivum L.) plants-regenerants produced by anther culture method from hybrids involved in the Latvian wheat breeding programme was performed. Flow cytometry was used to test ploidy of 3×103 cells of each green plantregenerant, and universal retrotransposon based iPBS (inter primer binding sites) method were used to establish genetic diversity of plants-regenerants. Progenies of 13 genetically distant hybrids were involved in the study. Most of plants-regenerants have leaves with mixoploid cells. Seeds were formed only by plants-regenerants that had cells with (2n = 6x) ploidy. Majority of fertile plantsregenerants have more than 40 % of hexaploid (6x) cells. The percentage of 6x cells in plants-regenerants and diversity in cell ploidy demonstrated association with mother plant (hybrid) genotype. Percent of spontaneous diplodization was also genotype-dependent. In this experiment colchicine treatment had no significant influence on outcome of fertile plants- regenerants. New dominant allele in a hybrid plant was found in comparison with both parents what indicated possible retrotransposon moving. Genetic diversity of the plants-regenerants obtained in anther culture is a combination of parent’s allele segregation and somaclonal variation.https://vavilov.elpub.ru/jour/article/view/703anther culturedh-lineswheat breedingploidyflow cytometryipbs |
| spellingShingle | D. Grauda K. Žagata G. Lanka V. Strazdina V. Fetere N. Lisina N. Krasnevska O. Fokina A. Mikelsone R. Ornicans I. Belogrudova I. Rashal Genetic diversity of wheat (Triticum aestivum L.) plants-regenerants produced by anther culture Вавиловский журнал генетики и селекции anther culture dh-lines wheat breeding ploidy flow cytometry ipbs |
| title | Genetic diversity of wheat (Triticum aestivum L.) plants-regenerants produced by anther culture |
| title_full | Genetic diversity of wheat (Triticum aestivum L.) plants-regenerants produced by anther culture |
| title_fullStr | Genetic diversity of wheat (Triticum aestivum L.) plants-regenerants produced by anther culture |
| title_full_unstemmed | Genetic diversity of wheat (Triticum aestivum L.) plants-regenerants produced by anther culture |
| title_short | Genetic diversity of wheat (Triticum aestivum L.) plants-regenerants produced by anther culture |
| title_sort | genetic diversity of wheat triticum aestivum l plants regenerants produced by anther culture |
| topic | anther culture dh-lines wheat breeding ploidy flow cytometry ipbs |
| url | https://vavilov.elpub.ru/jour/article/view/703 |
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