P311 Facilitates the Angiogenesis and Wound Healing Function of MSCs by Increasing VEGF Production
As a potential clinical therapeutic cell for injured tissue repair, mesenchymal stem cells (MSCs) have attracted increasing attention. Enhancing the pro-healing function of MSCs has gradually become an essential topic in improving the clinical efficacy of MSCs. Recently, studies have shown that neur...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.821932/full |
| _version_ | 1818332614412468224 |
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| author | Zhihui Liu Zhihui Liu Jiacai Yang Jiacai Yang Yunxia Chen Yunxia Chen Cheng Chen Cheng Chen Jue Wang Jue Wang Yew Mun Lee Yew Mun Lee Wenxia Zheng Wenxia Zheng Ruoyu Shang Ruoyu Shang Yuanyang Tang Yuanyang Tang Xiaorong Zhang Xiaorong Zhang Xiaohong Hu Xiaohong Hu Yong Huang Yong Huang Shiya Peng Yih-Cherng Liou Yih-Cherng Liou Weifeng He Weifeng He Gaoxing Luo Gaoxing Luo |
| author_facet | Zhihui Liu Zhihui Liu Jiacai Yang Jiacai Yang Yunxia Chen Yunxia Chen Cheng Chen Cheng Chen Jue Wang Jue Wang Yew Mun Lee Yew Mun Lee Wenxia Zheng Wenxia Zheng Ruoyu Shang Ruoyu Shang Yuanyang Tang Yuanyang Tang Xiaorong Zhang Xiaorong Zhang Xiaohong Hu Xiaohong Hu Yong Huang Yong Huang Shiya Peng Yih-Cherng Liou Yih-Cherng Liou Weifeng He Weifeng He Gaoxing Luo Gaoxing Luo |
| author_sort | Zhihui Liu |
| collection | DOAJ |
| description | As a potential clinical therapeutic cell for injured tissue repair, mesenchymal stem cells (MSCs) have attracted increasing attention. Enhancing the pro-healing function of MSCs has gradually become an essential topic in improving the clinical efficacy of MSCs. Recently, studies have shown that neuronal protein 3.1 (P311) plays a crucial role in promoting skin wound healing, suggesting P311 gene modification may improve the pro-healing function of MSCs. In this study, we demonstrated that increasing the in vivo expression of P311 could significantly enhance the ability of MSCs to lessen the number of inflammatory cells, increase the expression of IL10, reduce the levels of TNF-α and IFN-γ, increase collagen deposition, promote angiogenesis, and ultimately accelerate skin wound closure and improve the quality of wound healing. Importantly, we uncovered that P311 enhanced the pro-angiogenesis function of MSCs by increasing the production of vascular endothelial growth factor (VEGF) in vitro and in vivo. Mechanistically, we revealed that the mTOR signalling pathway was closely related to the regulation of P311 on VEGF production in MSCs. Together, our data displayed that P311 gene modification in MSCs augments their capabilities to promote skin wound closure, which might bring the dawn for its clinical application in the future. |
| first_indexed | 2024-12-13T13:38:33Z |
| format | Article |
| id | doaj.art-7c805fc704ad4ff684d9f4f8a46a9601 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-13T13:38:33Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-7c805fc704ad4ff684d9f4f8a46a96012022-12-21T23:43:39ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-01-011310.3389/fimmu.2022.821932821932P311 Facilitates the Angiogenesis and Wound Healing Function of MSCs by Increasing VEGF ProductionZhihui Liu0Zhihui Liu1Jiacai Yang2Jiacai Yang3Yunxia Chen4Yunxia Chen5Cheng Chen6Cheng Chen7Jue Wang8Jue Wang9Yew Mun Lee10Yew Mun Lee11Wenxia Zheng12Wenxia Zheng13Ruoyu Shang14Ruoyu Shang15Yuanyang Tang16Yuanyang Tang17Xiaorong Zhang18Xiaorong Zhang19Xiaohong Hu20Xiaohong Hu21Yong Huang22Yong Huang23Shiya Peng24Yih-Cherng Liou25Yih-Cherng Liou26Weifeng He27Weifeng He28Gaoxing Luo29Gaoxing Luo30State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, ChinaDepartment of Disease Proteomics, Chongqing Key Laboratory for Disease Proteomics, Chongqing, ChinaState Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, ChinaDepartment of Disease Proteomics, Chongqing Key Laboratory for Disease Proteomics, Chongqing, ChinaState Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, ChinaDepartment of Disease Proteomics, Chongqing Key Laboratory for Disease Proteomics, Chongqing, ChinaState Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, ChinaDepartment of Disease Proteomics, Chongqing Key Laboratory for Disease Proteomics, Chongqing, ChinaState Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, ChinaDepartment of Disease Proteomics, Chongqing Key Laboratory for Disease Proteomics, Chongqing, ChinaDepartment of Biological Sciences, Faculty of Science, National University of Singapore, Singapore, SingaporeNational University of Singapore (NUS) Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, SingaporeState Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, ChinaDepartment of Disease Proteomics, Chongqing Key Laboratory for Disease Proteomics, Chongqing, ChinaState Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, ChinaDepartment of Disease Proteomics, Chongqing Key Laboratory for Disease Proteomics, Chongqing, ChinaState Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, ChinaAcademy of Biological Engineering, Chongqing University, Chongqing, ChinaState Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, ChinaDepartment of Disease Proteomics, Chongqing Key Laboratory for Disease Proteomics, Chongqing, ChinaState Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, ChinaDepartment of Disease Proteomics, Chongqing Key Laboratory for Disease Proteomics, Chongqing, ChinaState Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, ChinaDepartment of Disease Proteomics, Chongqing Key Laboratory for Disease Proteomics, Chongqing, ChinaDepartment of Dermatology, Xinqiao Hospital, Army Military Medical University, Chongqing, ChinaDepartment of Biological Sciences, Faculty of Science, National University of Singapore, Singapore, SingaporeNational University of Singapore (NUS) Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, SingaporeState Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, ChinaDepartment of Disease Proteomics, Chongqing Key Laboratory for Disease Proteomics, Chongqing, ChinaState Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, ChinaDepartment of Disease Proteomics, Chongqing Key Laboratory for Disease Proteomics, Chongqing, ChinaAs a potential clinical therapeutic cell for injured tissue repair, mesenchymal stem cells (MSCs) have attracted increasing attention. Enhancing the pro-healing function of MSCs has gradually become an essential topic in improving the clinical efficacy of MSCs. Recently, studies have shown that neuronal protein 3.1 (P311) plays a crucial role in promoting skin wound healing, suggesting P311 gene modification may improve the pro-healing function of MSCs. In this study, we demonstrated that increasing the in vivo expression of P311 could significantly enhance the ability of MSCs to lessen the number of inflammatory cells, increase the expression of IL10, reduce the levels of TNF-α and IFN-γ, increase collagen deposition, promote angiogenesis, and ultimately accelerate skin wound closure and improve the quality of wound healing. Importantly, we uncovered that P311 enhanced the pro-angiogenesis function of MSCs by increasing the production of vascular endothelial growth factor (VEGF) in vitro and in vivo. Mechanistically, we revealed that the mTOR signalling pathway was closely related to the regulation of P311 on VEGF production in MSCs. Together, our data displayed that P311 gene modification in MSCs augments their capabilities to promote skin wound closure, which might bring the dawn for its clinical application in the future.https://www.frontiersin.org/articles/10.3389/fimmu.2022.821932/fullP311MSCsVEGFangiogenesiswound healingm-TOR signaling pathway |
| spellingShingle | Zhihui Liu Zhihui Liu Jiacai Yang Jiacai Yang Yunxia Chen Yunxia Chen Cheng Chen Cheng Chen Jue Wang Jue Wang Yew Mun Lee Yew Mun Lee Wenxia Zheng Wenxia Zheng Ruoyu Shang Ruoyu Shang Yuanyang Tang Yuanyang Tang Xiaorong Zhang Xiaorong Zhang Xiaohong Hu Xiaohong Hu Yong Huang Yong Huang Shiya Peng Yih-Cherng Liou Yih-Cherng Liou Weifeng He Weifeng He Gaoxing Luo Gaoxing Luo P311 Facilitates the Angiogenesis and Wound Healing Function of MSCs by Increasing VEGF Production Frontiers in Immunology P311 MSCs VEGF angiogenesis wound healing m-TOR signaling pathway |
| title | P311 Facilitates the Angiogenesis and Wound Healing Function of MSCs by Increasing VEGF Production |
| title_full | P311 Facilitates the Angiogenesis and Wound Healing Function of MSCs by Increasing VEGF Production |
| title_fullStr | P311 Facilitates the Angiogenesis and Wound Healing Function of MSCs by Increasing VEGF Production |
| title_full_unstemmed | P311 Facilitates the Angiogenesis and Wound Healing Function of MSCs by Increasing VEGF Production |
| title_short | P311 Facilitates the Angiogenesis and Wound Healing Function of MSCs by Increasing VEGF Production |
| title_sort | p311 facilitates the angiogenesis and wound healing function of mscs by increasing vegf production |
| topic | P311 MSCs VEGF angiogenesis wound healing m-TOR signaling pathway |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.821932/full |
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