H1N1 exposure during the convalescent stage of SARS-CoV-2 infection results in enhanced lung pathologic damage in hACE2 transgenic mice
The risk of secondary infection with SARS-CoV-2 and influenza A virus is becoming a practical problem that must be addressed as the flu season merges with the COVID-19 pandemic. As SARS-CoV-2 and influenza A virus have been found in patients, understanding the in vivo characteristics of the secondar...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2021.1938241 |
| _version_ | 1818498926559363072 |
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| author | Heng Li Xin Zhao Yurong Zhao Jing Li Huiwen Zheng Mengyi Xue Lei Guo Jian Zhou Jinling Yang Yuanyuan Zuo Yanli Chen Zening Yang Qiqi Fan Li Qin Haijing Shi Longding Liu |
| author_facet | Heng Li Xin Zhao Yurong Zhao Jing Li Huiwen Zheng Mengyi Xue Lei Guo Jian Zhou Jinling Yang Yuanyuan Zuo Yanli Chen Zening Yang Qiqi Fan Li Qin Haijing Shi Longding Liu |
| author_sort | Heng Li |
| collection | DOAJ |
| description | The risk of secondary infection with SARS-CoV-2 and influenza A virus is becoming a practical problem that must be addressed as the flu season merges with the COVID-19 pandemic. As SARS-CoV-2 and influenza A virus have been found in patients, understanding the in vivo characteristics of the secondary infection between these two viruses is a high priority. Here, hACE2 transgenic mice were challenged with the H1N1 virus at a nonlethal dose during the convalescent stage on 7 and 14 days post SARS-CoV-2 infection, and importantly, subsequent H1N1 infection showed enhanced viral shedding and virus tissue distribution. Histopathological observation revealed an extensive pathological change in the lungs related to H1N1 infection in mice recovered from SARS-CoV-2 infection, with severe inflammation infiltration and bronchiole disruption. Moreover, upon H1N1 exposure on 7 and 14 dpi of SARS-CoV-2 infection, the lymphocyte population activated at a lower level with T cell suppressed in both PBMC and lung. These findings will be valuable for evaluating antiviral therapeutics and vaccines as well as guiding public health work. |
| first_indexed | 2024-12-10T20:22:12Z |
| format | Article |
| id | doaj.art-7c8112cc888f4d86ab9228fea18bcb7a |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-12-10T20:22:12Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-7c8112cc888f4d86ab9228fea18bcb7a2022-12-22T01:34:59ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512021-01-011011156116810.1080/22221751.2021.1938241H1N1 exposure during the convalescent stage of SARS-CoV-2 infection results in enhanced lung pathologic damage in hACE2 transgenic miceHeng Li0Xin Zhao1Yurong Zhao2Jing Li3Huiwen Zheng4Mengyi Xue5Lei Guo6Jian Zhou7Jinling Yang8Yuanyuan Zuo9Yanli Chen10Zening Yang11Qiqi Fan12Li Qin13Haijing Shi14Longding Liu15Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, People’s Republic of ChinaThe risk of secondary infection with SARS-CoV-2 and influenza A virus is becoming a practical problem that must be addressed as the flu season merges with the COVID-19 pandemic. As SARS-CoV-2 and influenza A virus have been found in patients, understanding the in vivo characteristics of the secondary infection between these two viruses is a high priority. Here, hACE2 transgenic mice were challenged with the H1N1 virus at a nonlethal dose during the convalescent stage on 7 and 14 days post SARS-CoV-2 infection, and importantly, subsequent H1N1 infection showed enhanced viral shedding and virus tissue distribution. Histopathological observation revealed an extensive pathological change in the lungs related to H1N1 infection in mice recovered from SARS-CoV-2 infection, with severe inflammation infiltration and bronchiole disruption. Moreover, upon H1N1 exposure on 7 and 14 dpi of SARS-CoV-2 infection, the lymphocyte population activated at a lower level with T cell suppressed in both PBMC and lung. These findings will be valuable for evaluating antiviral therapeutics and vaccines as well as guiding public health work.https://www.tandfonline.com/doi/10.1080/22221751.2021.1938241SARS-CoV-2H1N1alternate infectionconvalescent stagehACE2 transgenic mice |
| spellingShingle | Heng Li Xin Zhao Yurong Zhao Jing Li Huiwen Zheng Mengyi Xue Lei Guo Jian Zhou Jinling Yang Yuanyuan Zuo Yanli Chen Zening Yang Qiqi Fan Li Qin Haijing Shi Longding Liu H1N1 exposure during the convalescent stage of SARS-CoV-2 infection results in enhanced lung pathologic damage in hACE2 transgenic mice Emerging Microbes and Infections SARS-CoV-2 H1N1 alternate infection convalescent stage hACE2 transgenic mice |
| title | H1N1 exposure during the convalescent stage of SARS-CoV-2 infection results in enhanced lung pathologic damage in hACE2 transgenic mice |
| title_full | H1N1 exposure during the convalescent stage of SARS-CoV-2 infection results in enhanced lung pathologic damage in hACE2 transgenic mice |
| title_fullStr | H1N1 exposure during the convalescent stage of SARS-CoV-2 infection results in enhanced lung pathologic damage in hACE2 transgenic mice |
| title_full_unstemmed | H1N1 exposure during the convalescent stage of SARS-CoV-2 infection results in enhanced lung pathologic damage in hACE2 transgenic mice |
| title_short | H1N1 exposure during the convalescent stage of SARS-CoV-2 infection results in enhanced lung pathologic damage in hACE2 transgenic mice |
| title_sort | h1n1 exposure during the convalescent stage of sars cov 2 infection results in enhanced lung pathologic damage in hace2 transgenic mice |
| topic | SARS-CoV-2 H1N1 alternate infection convalescent stage hACE2 transgenic mice |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2021.1938241 |
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