Biochemical and Metabolomic Responses of Antarctic Bacterium <i>Planococcus</i> sp. O5 Induced by Copper Ion
Heavy metal pollution in the Antarctic has gone beyond our imagination. Copper toxicity is a selective pressure on <i>Planococcus</i> sp. O5. We observed relatively broad tolerance in the polar bacterium. The heavy metal resistance pattern is Pb<sup>2+</sup> > Cu<sup>...
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MDPI AG
2022-06-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/10/6/302 |
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| author | Ziyi Cheng Cuijuan Shi Xiujun Gao Xiaofei Wang Guangfeng Kan |
| author_facet | Ziyi Cheng Cuijuan Shi Xiujun Gao Xiaofei Wang Guangfeng Kan |
| author_sort | Ziyi Cheng |
| collection | DOAJ |
| description | Heavy metal pollution in the Antarctic has gone beyond our imagination. Copper toxicity is a selective pressure on <i>Planococcus</i> sp. O5. We observed relatively broad tolerance in the polar bacterium. The heavy metal resistance pattern is Pb<sup>2+</sup> > Cu<sup>2+</sup> > Cd<sup>2+</sup> > Hg<sup>2+</sup> > Zn<sup>2+</sup>. In the study, we combined biochemical and metabolomics approaches to investigate the Cu<sup>2+</sup> adaptation mechanisms of the Antarctic bacterium. Biochemical analysis revealed that copper treatment elevated the activity of antioxidants and enzymes, maintaining the bacterial redox state balance and normal cell division and growth. Metabolomics analysis demonstrated that fatty acids, amino acids, and carbohydrates played dominant roles in copper stress adaptation. The findings suggested that the adaptive mechanisms of strain O5 to copper stress included protein synthesis and repair, accumulation of organic permeable substances, up-regulation of energy metabolism, and the formation of fatty acids. |
| first_indexed | 2024-03-09T22:20:15Z |
| format | Article |
| id | doaj.art-7c8873a337444bb6a152ea200b5bc59d |
| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-09T22:20:15Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxics |
| spelling | doaj.art-7c8873a337444bb6a152ea200b5bc59d2023-11-23T19:15:59ZengMDPI AGToxics2305-63042022-06-0110630210.3390/toxics10060302Biochemical and Metabolomic Responses of Antarctic Bacterium <i>Planococcus</i> sp. O5 Induced by Copper IonZiyi Cheng0Cuijuan Shi1Xiujun Gao2Xiaofei Wang3Guangfeng Kan4School of Marine Science and Technology, Harbin Institute of Technology, Huancui District, Weihai 264209, ChinaSchool of Marine Science and Technology, Harbin Institute of Technology, Huancui District, Weihai 264209, ChinaSchool of Marine Science and Technology, Harbin Institute of Technology, Huancui District, Weihai 264209, ChinaSchool of Marine Science and Technology, Harbin Institute of Technology, Huancui District, Weihai 264209, ChinaSchool of Marine Science and Technology, Harbin Institute of Technology, Huancui District, Weihai 264209, ChinaHeavy metal pollution in the Antarctic has gone beyond our imagination. Copper toxicity is a selective pressure on <i>Planococcus</i> sp. O5. We observed relatively broad tolerance in the polar bacterium. The heavy metal resistance pattern is Pb<sup>2+</sup> > Cu<sup>2+</sup> > Cd<sup>2+</sup> > Hg<sup>2+</sup> > Zn<sup>2+</sup>. In the study, we combined biochemical and metabolomics approaches to investigate the Cu<sup>2+</sup> adaptation mechanisms of the Antarctic bacterium. Biochemical analysis revealed that copper treatment elevated the activity of antioxidants and enzymes, maintaining the bacterial redox state balance and normal cell division and growth. Metabolomics analysis demonstrated that fatty acids, amino acids, and carbohydrates played dominant roles in copper stress adaptation. The findings suggested that the adaptive mechanisms of strain O5 to copper stress included protein synthesis and repair, accumulation of organic permeable substances, up-regulation of energy metabolism, and the formation of fatty acids.https://www.mdpi.com/2305-6304/10/6/302Antarctic straincopper stressadaption responsesmetabolomics |
| spellingShingle | Ziyi Cheng Cuijuan Shi Xiujun Gao Xiaofei Wang Guangfeng Kan Biochemical and Metabolomic Responses of Antarctic Bacterium <i>Planococcus</i> sp. O5 Induced by Copper Ion Toxics Antarctic strain copper stress adaption responses metabolomics |
| title | Biochemical and Metabolomic Responses of Antarctic Bacterium <i>Planococcus</i> sp. O5 Induced by Copper Ion |
| title_full | Biochemical and Metabolomic Responses of Antarctic Bacterium <i>Planococcus</i> sp. O5 Induced by Copper Ion |
| title_fullStr | Biochemical and Metabolomic Responses of Antarctic Bacterium <i>Planococcus</i> sp. O5 Induced by Copper Ion |
| title_full_unstemmed | Biochemical and Metabolomic Responses of Antarctic Bacterium <i>Planococcus</i> sp. O5 Induced by Copper Ion |
| title_short | Biochemical and Metabolomic Responses of Antarctic Bacterium <i>Planococcus</i> sp. O5 Induced by Copper Ion |
| title_sort | biochemical and metabolomic responses of antarctic bacterium i planococcus i sp o5 induced by copper ion |
| topic | Antarctic strain copper stress adaption responses metabolomics |
| url | https://www.mdpi.com/2305-6304/10/6/302 |
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