Electrochemical Sensing of Amoxicillin Drug-Assisted Uropathogenic <i>E. coli</i> Bacteria Using Gold Nanostructures—A Preliminary Study
The present study focuses on the electrochemical sensing of amoxicillin (AMX, as a model antibiotic drug) and its interaction with Uropathogenic <i>E. coli</i> (UPEC) bacteria (as a model pathogen) under physiological conditions. The electrochemical sensor probe is formulated by nanostru...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Electrochem |
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| Online Access: | https://www.mdpi.com/2673-3293/4/2/20 |
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| author | Jayaprakash Sushmitha Subramanian Nellaiappan |
| author_facet | Jayaprakash Sushmitha Subramanian Nellaiappan |
| author_sort | Jayaprakash Sushmitha |
| collection | DOAJ |
| description | The present study focuses on the electrochemical sensing of amoxicillin (AMX, as a model antibiotic drug) and its interaction with Uropathogenic <i>E. coli</i> (UPEC) bacteria (as a model pathogen) under physiological conditions. The electrochemical sensor probe is formulated by nanostructured gold wires (AuNWs) embedded in a carbon nanofiber–chitosan (CNF-CHIT) matrix. The synthesis of AuNWs is characterized by scanning electron microscopy (SEM), UV-Visible spectrophotometry, and X-ray photoelectron spectroscopy (XPS). The CNF-CHIT/AuNW-modified system is characterized by SEM and XPS. Initially, the CNF-CHIT/AuNW electrode was utilized for the sensing of AMX; later, in the antibiotic drug-assisted sensing of UPEC, i.e., in the presence of AMX, the interaction of UPEC was studied. The modified electrode showed appreciable sensitivity for AMX sensing; also, the interaction of AMX with UPEC is studied at two different conditions. One, at a fixed concentration of AMX (100 µM) and different concentrations of UPEC bacteria (0.6–1.2 × 10<sup>6</sup> CFU/mL), and another with incubation time (1 h–1 h 35 min) for bacterial reaction. The electrochemical antimicrobial resistance developed by UPEC, which is inherent in the sensing of AMX, is the key concept for the detection of pathogens. |
| first_indexed | 2024-03-11T02:32:46Z |
| format | Article |
| id | doaj.art-7c8c0523511a4db7af42313e96060de2 |
| institution | Directory Open Access Journal |
| issn | 2673-3293 |
| language | English |
| last_indexed | 2024-03-11T02:32:46Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Electrochem |
| spelling | doaj.art-7c8c0523511a4db7af42313e96060de22023-11-18T10:07:19ZengMDPI AGElectrochem2673-32932023-06-014229130010.3390/electrochem4020020Electrochemical Sensing of Amoxicillin Drug-Assisted Uropathogenic <i>E. coli</i> Bacteria Using Gold Nanostructures—A Preliminary StudyJayaprakash Sushmitha0Subramanian Nellaiappan1School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur 613401, Tamil Nadu, IndiaSchool of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur 613401, Tamil Nadu, IndiaThe present study focuses on the electrochemical sensing of amoxicillin (AMX, as a model antibiotic drug) and its interaction with Uropathogenic <i>E. coli</i> (UPEC) bacteria (as a model pathogen) under physiological conditions. The electrochemical sensor probe is formulated by nanostructured gold wires (AuNWs) embedded in a carbon nanofiber–chitosan (CNF-CHIT) matrix. The synthesis of AuNWs is characterized by scanning electron microscopy (SEM), UV-Visible spectrophotometry, and X-ray photoelectron spectroscopy (XPS). The CNF-CHIT/AuNW-modified system is characterized by SEM and XPS. Initially, the CNF-CHIT/AuNW electrode was utilized for the sensing of AMX; later, in the antibiotic drug-assisted sensing of UPEC, i.e., in the presence of AMX, the interaction of UPEC was studied. The modified electrode showed appreciable sensitivity for AMX sensing; also, the interaction of AMX with UPEC is studied at two different conditions. One, at a fixed concentration of AMX (100 µM) and different concentrations of UPEC bacteria (0.6–1.2 × 10<sup>6</sup> CFU/mL), and another with incubation time (1 h–1 h 35 min) for bacterial reaction. The electrochemical antimicrobial resistance developed by UPEC, which is inherent in the sensing of AMX, is the key concept for the detection of pathogens.https://www.mdpi.com/2673-3293/4/2/20urinary tract infectiongold nanostructuresamoxicillinuropathogenic <i>E. coli</i>electrochemical sensor |
| spellingShingle | Jayaprakash Sushmitha Subramanian Nellaiappan Electrochemical Sensing of Amoxicillin Drug-Assisted Uropathogenic <i>E. coli</i> Bacteria Using Gold Nanostructures—A Preliminary Study Electrochem urinary tract infection gold nanostructures amoxicillin uropathogenic <i>E. coli</i> electrochemical sensor |
| title | Electrochemical Sensing of Amoxicillin Drug-Assisted Uropathogenic <i>E. coli</i> Bacteria Using Gold Nanostructures—A Preliminary Study |
| title_full | Electrochemical Sensing of Amoxicillin Drug-Assisted Uropathogenic <i>E. coli</i> Bacteria Using Gold Nanostructures—A Preliminary Study |
| title_fullStr | Electrochemical Sensing of Amoxicillin Drug-Assisted Uropathogenic <i>E. coli</i> Bacteria Using Gold Nanostructures—A Preliminary Study |
| title_full_unstemmed | Electrochemical Sensing of Amoxicillin Drug-Assisted Uropathogenic <i>E. coli</i> Bacteria Using Gold Nanostructures—A Preliminary Study |
| title_short | Electrochemical Sensing of Amoxicillin Drug-Assisted Uropathogenic <i>E. coli</i> Bacteria Using Gold Nanostructures—A Preliminary Study |
| title_sort | electrochemical sensing of amoxicillin drug assisted uropathogenic i e coli i bacteria using gold nanostructures a preliminary study |
| topic | urinary tract infection gold nanostructures amoxicillin uropathogenic <i>E. coli</i> electrochemical sensor |
| url | https://www.mdpi.com/2673-3293/4/2/20 |
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