The VvWRKY37 Regulates Bud Break in Grape Vine Through ABA-Mediated Signaling Pathways
Dormancy is a common survival strategy in plants to temporarily suspend visible growth under unsuitable conditions. The elaborate mechanism underlying bud break in perennial woody plants is gradually illustrated. Here, we identified a grape vine WRKY transcription factor, VvWRKY37, which was highly...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-06-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.929892/full |
| _version_ | 1818552455452950528 |
|---|---|
| author | Feng-Pan Wang Feng-Pan Wang Pan-Pan Zhao Lei Zhang Heng Zhai Muhammad Abid Yuan-Peng Du |
| author_facet | Feng-Pan Wang Feng-Pan Wang Pan-Pan Zhao Lei Zhang Heng Zhai Muhammad Abid Yuan-Peng Du |
| author_sort | Feng-Pan Wang |
| collection | DOAJ |
| description | Dormancy is a common survival strategy in plants to temporarily suspend visible growth under unsuitable conditions. The elaborate mechanism underlying bud break in perennial woody plants is gradually illustrated. Here, we identified a grape vine WRKY transcription factor, VvWRKY37, which was highly expressed in dormant buds. It was particularly induced by the application of exogenous abscisic acid, and depressed on exposure to gibberellin and low temperature (4°C) stress at the transcript level. The yeast one-hybrid assay confirmed that VvWRKY37 had a transcriptional activity. Ectopic over-expression of VvWRKY37 significantly delayed bud break of transgenic poplar plants. As an ABA-inducible gene, VvWRKY37 also depressed the expression of ABA catabolic gene CYP707As and enhanced the accumulation of endogenous ABA in transgenic poplar plants. The molecular pieces of evidence showed that VvWRKY37 preferentially recognized and bound W-box 5′-G/CATTGACT/C/G-3′ cis-element in vitro. Additionally, VvABI5 and VvABF2 acted as the upstream transcriptional activators of VvWRKY37 via protein-DNA interactions. Taken together, our findings provided valuable insights into a new regulatory mechanism of WRKY TF by which it modulates bud break through ABA-mediated signaling pathways. |
| first_indexed | 2024-12-12T09:13:25Z |
| format | Article |
| id | doaj.art-7c8df0d0c0704952be4709a877344410 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T09:13:25Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-7c8df0d0c0704952be4709a8773444102022-12-22T00:29:27ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-06-011310.3389/fpls.2022.929892929892The VvWRKY37 Regulates Bud Break in Grape Vine Through ABA-Mediated Signaling PathwaysFeng-Pan Wang0Feng-Pan Wang1Pan-Pan Zhao2Lei Zhang3Heng Zhai4Muhammad Abid5Yuan-Peng Du6Lushan Botanical Garden, Chinese Academy of Sciences, Jiujiang, ChinaNational Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, ChinaGuangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaCollege of Biological and Enology Engineering, Taishan University, Tai’an, ChinaNational Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, ChinaLushan Botanical Garden, Chinese Academy of Sciences, Jiujiang, ChinaNational Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, ChinaDormancy is a common survival strategy in plants to temporarily suspend visible growth under unsuitable conditions. The elaborate mechanism underlying bud break in perennial woody plants is gradually illustrated. Here, we identified a grape vine WRKY transcription factor, VvWRKY37, which was highly expressed in dormant buds. It was particularly induced by the application of exogenous abscisic acid, and depressed on exposure to gibberellin and low temperature (4°C) stress at the transcript level. The yeast one-hybrid assay confirmed that VvWRKY37 had a transcriptional activity. Ectopic over-expression of VvWRKY37 significantly delayed bud break of transgenic poplar plants. As an ABA-inducible gene, VvWRKY37 also depressed the expression of ABA catabolic gene CYP707As and enhanced the accumulation of endogenous ABA in transgenic poplar plants. The molecular pieces of evidence showed that VvWRKY37 preferentially recognized and bound W-box 5′-G/CATTGACT/C/G-3′ cis-element in vitro. Additionally, VvABI5 and VvABF2 acted as the upstream transcriptional activators of VvWRKY37 via protein-DNA interactions. Taken together, our findings provided valuable insights into a new regulatory mechanism of WRKY TF by which it modulates bud break through ABA-mediated signaling pathways.https://www.frontiersin.org/articles/10.3389/fpls.2022.929892/fullgrapevinebud breakWRKYabscisic acidtranscriptional regulation |
| spellingShingle | Feng-Pan Wang Feng-Pan Wang Pan-Pan Zhao Lei Zhang Heng Zhai Muhammad Abid Yuan-Peng Du The VvWRKY37 Regulates Bud Break in Grape Vine Through ABA-Mediated Signaling Pathways Frontiers in Plant Science grapevine bud break WRKY abscisic acid transcriptional regulation |
| title | The VvWRKY37 Regulates Bud Break in Grape Vine Through ABA-Mediated Signaling Pathways |
| title_full | The VvWRKY37 Regulates Bud Break in Grape Vine Through ABA-Mediated Signaling Pathways |
| title_fullStr | The VvWRKY37 Regulates Bud Break in Grape Vine Through ABA-Mediated Signaling Pathways |
| title_full_unstemmed | The VvWRKY37 Regulates Bud Break in Grape Vine Through ABA-Mediated Signaling Pathways |
| title_short | The VvWRKY37 Regulates Bud Break in Grape Vine Through ABA-Mediated Signaling Pathways |
| title_sort | vvwrky37 regulates bud break in grape vine through aba mediated signaling pathways |
| topic | grapevine bud break WRKY abscisic acid transcriptional regulation |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.929892/full |
| work_keys_str_mv | AT fengpanwang thevvwrky37regulatesbudbreakingrapevinethroughabamediatedsignalingpathways AT fengpanwang thevvwrky37regulatesbudbreakingrapevinethroughabamediatedsignalingpathways AT panpanzhao thevvwrky37regulatesbudbreakingrapevinethroughabamediatedsignalingpathways AT leizhang thevvwrky37regulatesbudbreakingrapevinethroughabamediatedsignalingpathways AT hengzhai thevvwrky37regulatesbudbreakingrapevinethroughabamediatedsignalingpathways AT muhammadabid thevvwrky37regulatesbudbreakingrapevinethroughabamediatedsignalingpathways AT yuanpengdu thevvwrky37regulatesbudbreakingrapevinethroughabamediatedsignalingpathways AT fengpanwang vvwrky37regulatesbudbreakingrapevinethroughabamediatedsignalingpathways AT fengpanwang vvwrky37regulatesbudbreakingrapevinethroughabamediatedsignalingpathways AT panpanzhao vvwrky37regulatesbudbreakingrapevinethroughabamediatedsignalingpathways AT leizhang vvwrky37regulatesbudbreakingrapevinethroughabamediatedsignalingpathways AT hengzhai vvwrky37regulatesbudbreakingrapevinethroughabamediatedsignalingpathways AT muhammadabid vvwrky37regulatesbudbreakingrapevinethroughabamediatedsignalingpathways AT yuanpengdu vvwrky37regulatesbudbreakingrapevinethroughabamediatedsignalingpathways |