A Biological Study of Composites Based on the Blends of Nanohydroxyapatite, Silk Fibroin and Chitosan
In this work, the biological properties of three-dimensional scaffolds based on a blend of nanohydroxyapatite (nHA), silk fibroin (SF), and chitosan (CTS), were prepared using a lyophilization technique with various weight ratios: 10:45:45, 15:15:70, 15:70:15, 20:40:40, 40:30:30, and 70:15:15 nHA:SF...
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2022-08-01
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| Online Access: | https://www.mdpi.com/1996-1944/15/15/5444 |
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| author | Anna Tuwalska Alina Sionkowska Amadeusz Bryła Grzegorz Tylko Anna Maria Osyczka Michele Laus Lucy Vojtová |
| author_facet | Anna Tuwalska Alina Sionkowska Amadeusz Bryła Grzegorz Tylko Anna Maria Osyczka Michele Laus Lucy Vojtová |
| author_sort | Anna Tuwalska |
| collection | DOAJ |
| description | In this work, the biological properties of three-dimensional scaffolds based on a blend of nanohydroxyapatite (nHA), silk fibroin (SF), and chitosan (CTS), were prepared using a lyophilization technique with various weight ratios: 10:45:45, 15:15:70, 15:70:15, 20:40:40, 40:30:30, and 70:15:15 nHA:SF:CTS, respectively. The basic 3D scaffolds were obtained from 5% (<i>w</i>/<i>w</i>) chitosan and 5% silk fibroin solutions and then nHA was added. The morphology and physicochemical properties of scaffolds were studied and compared. A biological test was performed to study the growth and osteogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs). It was found that the addition of chitosan increases the resistance properties and extends the degradation time of materials. In vitro studies with human mesenchymal stem cells found a high degree of biotolerance for the materials produced, especially for the 20:40:40 and 15:70:15 (nHa:SF:CTS) ratios. The presence of silk fibroin and the elongated shape of the pores positively influenced the differentiation of cells into osteogenic cells. By taking advantage of the differentiation/proliferation cues offered by individual components, the composites based on the nanohydroxyapatite, silk fibroin, and chitosan scaffold may be suitable for bone tissue engineering, and possibly offer an alternative to the widespread use of collagen materials. |
| first_indexed | 2024-03-09T12:23:49Z |
| format | Article |
| id | doaj.art-7c92c1e38b9d4be692dbcb003bd2dd62 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T12:23:49Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-7c92c1e38b9d4be692dbcb003bd2dd622023-11-30T22:36:59ZengMDPI AGMaterials1996-19442022-08-011515544410.3390/ma15155444A Biological Study of Composites Based on the Blends of Nanohydroxyapatite, Silk Fibroin and ChitosanAnna Tuwalska0Alina Sionkowska1Amadeusz Bryła2Grzegorz Tylko3Anna Maria Osyczka4Michele Laus5Lucy Vojtová6Department of Biomaterials and Cosmetic Chemistry, Faculty of Chemistry, Nicolaus Copernicus University in Toruń, 87-100 Toruń, PolandDepartment of Biomaterials and Cosmetic Chemistry, Faculty of Chemistry, Nicolaus Copernicus University in Toruń, 87-100 Toruń, PolandInstitute of Environmental Science, Faculty of Biology, Jagiellonian University, 30-387 Kraków, PolandDepartment of Biology and Cell Imaging, Faculty of Biology, Institute of Zoology and Biomedical Research, Jagiellonian University, 30-387 Kraków, PolandDepartment of Biology and Cell Imaging, Faculty of Biology, Institute of Zoology and Biomedical Research, Jagiellonian University, 30-387 Kraków, PolandDepartment of Science and Technological Innovation, University of Eastern Piedmont “A. Avogadro”, 15121 Alessandria, ItalyCEITEC—Central European Institute of Technology, Brno University of Technology, 612 00 Brno, Czech RepublicIn this work, the biological properties of three-dimensional scaffolds based on a blend of nanohydroxyapatite (nHA), silk fibroin (SF), and chitosan (CTS), were prepared using a lyophilization technique with various weight ratios: 10:45:45, 15:15:70, 15:70:15, 20:40:40, 40:30:30, and 70:15:15 nHA:SF:CTS, respectively. The basic 3D scaffolds were obtained from 5% (<i>w</i>/<i>w</i>) chitosan and 5% silk fibroin solutions and then nHA was added. The morphology and physicochemical properties of scaffolds were studied and compared. A biological test was performed to study the growth and osteogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs). It was found that the addition of chitosan increases the resistance properties and extends the degradation time of materials. In vitro studies with human mesenchymal stem cells found a high degree of biotolerance for the materials produced, especially for the 20:40:40 and 15:70:15 (nHa:SF:CTS) ratios. The presence of silk fibroin and the elongated shape of the pores positively influenced the differentiation of cells into osteogenic cells. By taking advantage of the differentiation/proliferation cues offered by individual components, the composites based on the nanohydroxyapatite, silk fibroin, and chitosan scaffold may be suitable for bone tissue engineering, and possibly offer an alternative to the widespread use of collagen materials.https://www.mdpi.com/1996-1944/15/15/5444silk fibroinchitosanhydroxyapatitebiological studymesenchymal stem cellsbone regeneration |
| spellingShingle | Anna Tuwalska Alina Sionkowska Amadeusz Bryła Grzegorz Tylko Anna Maria Osyczka Michele Laus Lucy Vojtová A Biological Study of Composites Based on the Blends of Nanohydroxyapatite, Silk Fibroin and Chitosan Materials silk fibroin chitosan hydroxyapatite biological study mesenchymal stem cells bone regeneration |
| title | A Biological Study of Composites Based on the Blends of Nanohydroxyapatite, Silk Fibroin and Chitosan |
| title_full | A Biological Study of Composites Based on the Blends of Nanohydroxyapatite, Silk Fibroin and Chitosan |
| title_fullStr | A Biological Study of Composites Based on the Blends of Nanohydroxyapatite, Silk Fibroin and Chitosan |
| title_full_unstemmed | A Biological Study of Composites Based on the Blends of Nanohydroxyapatite, Silk Fibroin and Chitosan |
| title_short | A Biological Study of Composites Based on the Blends of Nanohydroxyapatite, Silk Fibroin and Chitosan |
| title_sort | biological study of composites based on the blends of nanohydroxyapatite silk fibroin and chitosan |
| topic | silk fibroin chitosan hydroxyapatite biological study mesenchymal stem cells bone regeneration |
| url | https://www.mdpi.com/1996-1944/15/15/5444 |
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