Association between human IL-10 gene polymorphisms and serum IL-10 level in patients with food allergy
Single nucleotide polymorphisms (SNPs) of the interleukin-10 (IL-10) gene are associated with allergic diseases in different populations. This study aimed to determine the distribution of two SNPs at –1082A/G and –592A/C (rs1800896 and rs1800872, respectively) in the IL-10 gene promoter of Taiwanese...
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| Format: | Article |
| Language: | English |
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Elsevier
2012-12-01
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| Series: | Journal of the Formosan Medical Association |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0929664612000897 |
| _version_ | 1819181605827117056 |
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| author | Tuo-Kang Chen Jyh-Hong Lee Hsin-Hui Yu Yao-Hsu Yang Li-Chieh Wang Yu-Tsan Lin Bor-Luen Chiang |
| author_facet | Tuo-Kang Chen Jyh-Hong Lee Hsin-Hui Yu Yao-Hsu Yang Li-Chieh Wang Yu-Tsan Lin Bor-Luen Chiang |
| author_sort | Tuo-Kang Chen |
| collection | DOAJ |
| description | Single nucleotide polymorphisms (SNPs) of the interleukin-10 (IL-10) gene are associated with allergic diseases in different populations. This study aimed to determine the distribution of two SNPs at –1082A/G and –592A/C (rs1800896 and rs1800872, respectively) in the IL-10 gene promoter of Taiwanese food allergy (FA) patients, and also to compare the serum IL-10 levels between patients with (FA) and controls.
Methods: Thirty-seven patients with FA and 52 controls were enrolled, and their peripheral blood was collected for IL-10 SNP genotyping and the corresponding serum IL-10 level of each genotype.
Results: The thirty-seven FA patients had positive food-specific IgE (≥ 0.75 kU/L) to more than one food, and the most frequent allergens wereshrimp and crab (56.8% and 35.1%, respectively). The genotype distributions in the FA patients compared to the control group were AA and AG at –1082A/G (86.5% and 13.5% vs. 86.6% and 13.4%, respectively), and AA, AC, and CC at –592A/C (45.9%, 43.3% and 10.8% vs. 38.5%, 48.1% and 13.4%, respectively). Serum IL-10 levels were significantly lower in the FA group than in the control group (p=0.0187), and the IL-10 level of –592A/C of genotype AA was significantly lower than that of the other genotypes (AC+CC) (p=0.007). Patients with AA/AA haplotype homozygotes (10 of 24) had significantly lower serum IL-10 levels than those with other haplotypes.
Conclusions: The two SNPs at –1082A/G and –592A/C of IL-10 were associated with FA in our Taiwanese population, and FA patients with the genotype AA/AA haplotype homozygotes had lower serum IL-10 levels. This suggests that IL-10 might play a critical role in the pathogenesis of FA. We suggest that it may be practicable to evaluate the serum IL-10 levels of FA patients and to predict the possibility of FA if genotypes and haplotypes are checked regularly. |
| first_indexed | 2024-12-22T22:32:54Z |
| format | Article |
| id | doaj.art-7c94fd50fc034cba9695b6c8ef8eb518 |
| institution | Directory Open Access Journal |
| issn | 0929-6646 |
| language | English |
| last_indexed | 2024-12-22T22:32:54Z |
| publishDate | 2012-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of the Formosan Medical Association |
| spelling | doaj.art-7c94fd50fc034cba9695b6c8ef8eb5182022-12-21T18:10:24ZengElsevierJournal of the Formosan Medical Association0929-66462012-12-011111268669210.1016/j.jfma.2011.11.027Association between human IL-10 gene polymorphisms and serum IL-10 level in patients with food allergyTuo-Kang ChenJyh-Hong LeeHsin-Hui YuYao-Hsu YangLi-Chieh WangYu-Tsan LinBor-Luen ChiangSingle nucleotide polymorphisms (SNPs) of the interleukin-10 (IL-10) gene are associated with allergic diseases in different populations. This study aimed to determine the distribution of two SNPs at –1082A/G and –592A/C (rs1800896 and rs1800872, respectively) in the IL-10 gene promoter of Taiwanese food allergy (FA) patients, and also to compare the serum IL-10 levels between patients with (FA) and controls. Methods: Thirty-seven patients with FA and 52 controls were enrolled, and their peripheral blood was collected for IL-10 SNP genotyping and the corresponding serum IL-10 level of each genotype. Results: The thirty-seven FA patients had positive food-specific IgE (≥ 0.75 kU/L) to more than one food, and the most frequent allergens wereshrimp and crab (56.8% and 35.1%, respectively). The genotype distributions in the FA patients compared to the control group were AA and AG at –1082A/G (86.5% and 13.5% vs. 86.6% and 13.4%, respectively), and AA, AC, and CC at –592A/C (45.9%, 43.3% and 10.8% vs. 38.5%, 48.1% and 13.4%, respectively). Serum IL-10 levels were significantly lower in the FA group than in the control group (p=0.0187), and the IL-10 level of –592A/C of genotype AA was significantly lower than that of the other genotypes (AC+CC) (p=0.007). Patients with AA/AA haplotype homozygotes (10 of 24) had significantly lower serum IL-10 levels than those with other haplotypes. Conclusions: The two SNPs at –1082A/G and –592A/C of IL-10 were associated with FA in our Taiwanese population, and FA patients with the genotype AA/AA haplotype homozygotes had lower serum IL-10 levels. This suggests that IL-10 might play a critical role in the pathogenesis of FA. We suggest that it may be practicable to evaluate the serum IL-10 levels of FA patients and to predict the possibility of FA if genotypes and haplotypes are checked regularly.http://www.sciencedirect.com/science/article/pii/S0929664612000897interleukin-10food allergypolymorphismgene |
| spellingShingle | Tuo-Kang Chen Jyh-Hong Lee Hsin-Hui Yu Yao-Hsu Yang Li-Chieh Wang Yu-Tsan Lin Bor-Luen Chiang Association between human IL-10 gene polymorphisms and serum IL-10 level in patients with food allergy Journal of the Formosan Medical Association interleukin-10 food allergy polymorphism gene |
| title | Association between human IL-10 gene polymorphisms and serum IL-10 level in patients with food allergy |
| title_full | Association between human IL-10 gene polymorphisms and serum IL-10 level in patients with food allergy |
| title_fullStr | Association between human IL-10 gene polymorphisms and serum IL-10 level in patients with food allergy |
| title_full_unstemmed | Association between human IL-10 gene polymorphisms and serum IL-10 level in patients with food allergy |
| title_short | Association between human IL-10 gene polymorphisms and serum IL-10 level in patients with food allergy |
| title_sort | association between human il 10 gene polymorphisms and serum il 10 level in patients with food allergy |
| topic | interleukin-10 food allergy polymorphism gene |
| url | http://www.sciencedirect.com/science/article/pii/S0929664612000897 |
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