Lactate Sensors on Flexible Substrates
Lactate detection by an in situ sensor is of great need in clinical medicine, food processing, and athletic performance monitoring. In this paper, a flexible, easy to fabricate, and low-cost biosensor base on lactate oxidase is presented. The fabrication processes, including metal deposition, sol-ge...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2016-09-01
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| Series: | Biosensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2079-6374/6/3/48 |
| _version_ | 1811294996532297728 |
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| author | Xuesong Yang Timothy Fu Pavan Kumar Kota Maggie Tjia Cuong Manh Nguyen Jung-Chih Chiao |
| author_facet | Xuesong Yang Timothy Fu Pavan Kumar Kota Maggie Tjia Cuong Manh Nguyen Jung-Chih Chiao |
| author_sort | Xuesong Yang |
| collection | DOAJ |
| description | Lactate detection by an in situ sensor is of great need in clinical medicine, food processing, and athletic performance monitoring. In this paper, a flexible, easy to fabricate, and low-cost biosensor base on lactate oxidase is presented. The fabrication processes, including metal deposition, sol-gel IrOx deposition, and drop-dry enzyme loading method, are described in detail. The loaded enzyme was examined by scanning electron microscopy. Cyclic voltammetry was used to characterize the sensors. Durability, sensibility, and selectivity of the biosensors were examined. The comparison for different electrode sizes and different sensing film materials was conducted. The sensor could last for four weeks with an average surface area normalized sensitivity of 950 nA/(cm2 mM) and 9250 nA/(cm2 mM) for Au-based electrodes, and IrOx-modified electrodes respectively, both with an electrode size of 100 × 50 μm. The self-referencing method to record noises simultaneously with the working electrode greatly improved sensor sensitivity and selectivity. The sensor showed little response to interference chemicals, such as glutamate and dopamine. |
| first_indexed | 2024-04-13T05:25:46Z |
| format | Article |
| id | doaj.art-7c951883354e4cbe98b0227ffc5ad539 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-04-13T05:25:46Z |
| publishDate | 2016-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-7c951883354e4cbe98b0227ffc5ad5392022-12-22T03:00:35ZengMDPI AGBiosensors2079-63742016-09-01634810.3390/bios6030048bios6030048Lactate Sensors on Flexible SubstratesXuesong Yang0Timothy Fu1Pavan Kumar Kota2Maggie Tjia3Cuong Manh Nguyen4Jung-Chih Chiao5Electrical Engineering, University of Texas‒Arlington, Arlington, TX 76019, USATexas A&M Health Science Center, Bryan, TX 77807, USAElectrical Engineering, University of Texas‒Arlington, Arlington, TX 76019, USAElectrical Engineering, University of Texas‒Arlington, Arlington, TX 76019, USAElectrical Engineering, University of Texas‒Arlington, Arlington, TX 76019, USAElectrical Engineering, University of Texas‒Arlington, Arlington, TX 76019, USALactate detection by an in situ sensor is of great need in clinical medicine, food processing, and athletic performance monitoring. In this paper, a flexible, easy to fabricate, and low-cost biosensor base on lactate oxidase is presented. The fabrication processes, including metal deposition, sol-gel IrOx deposition, and drop-dry enzyme loading method, are described in detail. The loaded enzyme was examined by scanning electron microscopy. Cyclic voltammetry was used to characterize the sensors. Durability, sensibility, and selectivity of the biosensors were examined. The comparison for different electrode sizes and different sensing film materials was conducted. The sensor could last for four weeks with an average surface area normalized sensitivity of 950 nA/(cm2 mM) and 9250 nA/(cm2 mM) for Au-based electrodes, and IrOx-modified electrodes respectively, both with an electrode size of 100 × 50 μm. The self-referencing method to record noises simultaneously with the working electrode greatly improved sensor sensitivity and selectivity. The sensor showed little response to interference chemicals, such as glutamate and dopamine.http://www.mdpi.com/2079-6374/6/3/48flexible substratelactateIrOxbiosensor |
| spellingShingle | Xuesong Yang Timothy Fu Pavan Kumar Kota Maggie Tjia Cuong Manh Nguyen Jung-Chih Chiao Lactate Sensors on Flexible Substrates Biosensors flexible substrate lactate IrOx biosensor |
| title | Lactate Sensors on Flexible Substrates |
| title_full | Lactate Sensors on Flexible Substrates |
| title_fullStr | Lactate Sensors on Flexible Substrates |
| title_full_unstemmed | Lactate Sensors on Flexible Substrates |
| title_short | Lactate Sensors on Flexible Substrates |
| title_sort | lactate sensors on flexible substrates |
| topic | flexible substrate lactate IrOx biosensor |
| url | http://www.mdpi.com/2079-6374/6/3/48 |
| work_keys_str_mv | AT xuesongyang lactatesensorsonflexiblesubstrates AT timothyfu lactatesensorsonflexiblesubstrates AT pavankumarkota lactatesensorsonflexiblesubstrates AT maggietjia lactatesensorsonflexiblesubstrates AT cuongmanhnguyen lactatesensorsonflexiblesubstrates AT jungchihchiao lactatesensorsonflexiblesubstrates |