Amyloid-β Oligomer-Induced Electrophysiological Mechanisms and Electrical Impedance Changes in Neurons
Amyloid plays a critical role in the pathogenesis of Alzheimer’s disease (AD) and can aggregate to form oligomers and fibrils in the brain. There is increasing evidence that highly toxic amyloid-β oligomers (AβOs) lead to tau protein aggregation, hyperphosphorylation, neuroinflammation, neuronal los...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-02-01
|
| Series: | Sensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8220/24/4/1211 |
| _version_ | 1797297009528406016 |
|---|---|
| author | Shimeng Sun Qing Ma Qiyu Sheng Shangwei Huang Chenxia Wu Junsong Liu Jia Xu |
| author_facet | Shimeng Sun Qing Ma Qiyu Sheng Shangwei Huang Chenxia Wu Junsong Liu Jia Xu |
| author_sort | Shimeng Sun |
| collection | DOAJ |
| description | Amyloid plays a critical role in the pathogenesis of Alzheimer’s disease (AD) and can aggregate to form oligomers and fibrils in the brain. There is increasing evidence that highly toxic amyloid-β oligomers (AβOs) lead to tau protein aggregation, hyperphosphorylation, neuroinflammation, neuronal loss, synaptic loss, and dysfunction. Although the effects of AβOs on neurons have been investigated using conventional biochemical experiments, there are no established criteria for electrical evaluation. To this end, we explored electrophysiological changes in mouse hippocampal neurons (HT22) following exposure to AβOs and/or naringenin (Nar, a flavonoid compound) using electrical impedance spectroscopy (EIS). AβO-induced HT22 showed a decreased impedance amplitude and increased phase angle, and the addition of Nar reversed these changes. The characteristic frequency was markedly increased with AβO exposure, which was also reversed by Nar. The AβOs decreased intranuclear and cytoplasmic resistance and increased nucleus resistance and extracellular capacitance. Overall, the innovative construction of the eight-element CPE-equivalent circuit model further reflects that the pseudo-capacitance of the cell membrane and cell nucleus was increased in the AβO-induced group. This study conclusively revealed that AβOs induce cytotoxic effects by disrupting the resistance characteristics of unit membranes. The results further support that EIS is an effective technique for evaluating AβO-induced neuronal damage and microscopic electrical distinctions in the sub-microscopic structure of reactive cells. |
| first_indexed | 2024-03-07T22:15:01Z |
| format | Article |
| id | doaj.art-7c9716e3488f4fc7aca105d946a5c5ed |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-07T22:15:01Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-7c9716e3488f4fc7aca105d946a5c5ed2024-02-23T15:33:51ZengMDPI AGSensors1424-82202024-02-01244121110.3390/s24041211Amyloid-β Oligomer-Induced Electrophysiological Mechanisms and Electrical Impedance Changes in NeuronsShimeng Sun0Qing Ma1Qiyu Sheng2Shangwei Huang3Chenxia Wu4Junsong Liu5Jia Xu6Department of Physiology and Pharmacology, Health Science Center, Ningbo University, Ningbo 315211, ChinaDepartment of Physiology and Pharmacology, Health Science Center, Ningbo University, Ningbo 315211, ChinaDepartment of Physiology and Pharmacology, Health Science Center, Ningbo University, Ningbo 315211, ChinaDepartment of Physiology and Pharmacology, Health Science Center, Ningbo University, Ningbo 315211, ChinaDepartment of Physiology and Pharmacology, Health Science Center, Ningbo University, Ningbo 315211, ChinaState Key Laboratory of Superhard Materials, College of Physics, Jilin University, Changchun 130012, ChinaDepartment of Physiology and Pharmacology, Health Science Center, Ningbo University, Ningbo 315211, ChinaAmyloid plays a critical role in the pathogenesis of Alzheimer’s disease (AD) and can aggregate to form oligomers and fibrils in the brain. There is increasing evidence that highly toxic amyloid-β oligomers (AβOs) lead to tau protein aggregation, hyperphosphorylation, neuroinflammation, neuronal loss, synaptic loss, and dysfunction. Although the effects of AβOs on neurons have been investigated using conventional biochemical experiments, there are no established criteria for electrical evaluation. To this end, we explored electrophysiological changes in mouse hippocampal neurons (HT22) following exposure to AβOs and/or naringenin (Nar, a flavonoid compound) using electrical impedance spectroscopy (EIS). AβO-induced HT22 showed a decreased impedance amplitude and increased phase angle, and the addition of Nar reversed these changes. The characteristic frequency was markedly increased with AβO exposure, which was also reversed by Nar. The AβOs decreased intranuclear and cytoplasmic resistance and increased nucleus resistance and extracellular capacitance. Overall, the innovative construction of the eight-element CPE-equivalent circuit model further reflects that the pseudo-capacitance of the cell membrane and cell nucleus was increased in the AβO-induced group. This study conclusively revealed that AβOs induce cytotoxic effects by disrupting the resistance characteristics of unit membranes. The results further support that EIS is an effective technique for evaluating AβO-induced neuronal damage and microscopic electrical distinctions in the sub-microscopic structure of reactive cells.https://www.mdpi.com/1424-8220/24/4/1211Aβ oligomerAlzheimer’s diseaseelectrical impedance spectrumCPE-equivalent electrical circuit modelcytotoxicity |
| spellingShingle | Shimeng Sun Qing Ma Qiyu Sheng Shangwei Huang Chenxia Wu Junsong Liu Jia Xu Amyloid-β Oligomer-Induced Electrophysiological Mechanisms and Electrical Impedance Changes in Neurons Sensors Aβ oligomer Alzheimer’s disease electrical impedance spectrum CPE-equivalent electrical circuit model cytotoxicity |
| title | Amyloid-β Oligomer-Induced Electrophysiological Mechanisms and Electrical Impedance Changes in Neurons |
| title_full | Amyloid-β Oligomer-Induced Electrophysiological Mechanisms and Electrical Impedance Changes in Neurons |
| title_fullStr | Amyloid-β Oligomer-Induced Electrophysiological Mechanisms and Electrical Impedance Changes in Neurons |
| title_full_unstemmed | Amyloid-β Oligomer-Induced Electrophysiological Mechanisms and Electrical Impedance Changes in Neurons |
| title_short | Amyloid-β Oligomer-Induced Electrophysiological Mechanisms and Electrical Impedance Changes in Neurons |
| title_sort | amyloid β oligomer induced electrophysiological mechanisms and electrical impedance changes in neurons |
| topic | Aβ oligomer Alzheimer’s disease electrical impedance spectrum CPE-equivalent electrical circuit model cytotoxicity |
| url | https://www.mdpi.com/1424-8220/24/4/1211 |
| work_keys_str_mv | AT shimengsun amyloidboligomerinducedelectrophysiologicalmechanismsandelectricalimpedancechangesinneurons AT qingma amyloidboligomerinducedelectrophysiologicalmechanismsandelectricalimpedancechangesinneurons AT qiyusheng amyloidboligomerinducedelectrophysiologicalmechanismsandelectricalimpedancechangesinneurons AT shangweihuang amyloidboligomerinducedelectrophysiologicalmechanismsandelectricalimpedancechangesinneurons AT chenxiawu amyloidboligomerinducedelectrophysiologicalmechanismsandelectricalimpedancechangesinneurons AT junsongliu amyloidboligomerinducedelectrophysiologicalmechanismsandelectricalimpedancechangesinneurons AT jiaxu amyloidboligomerinducedelectrophysiologicalmechanismsandelectricalimpedancechangesinneurons |