3D-printed lightweight dorsal skin fold chambers from PEEK reduce chamber-related animal distress
Abstract The dorsal skinfold chamber is one of the most important in vivo models for repetitive longitudinal assessment of microcirculation and inflammation. This study aimed to refine this model by introducing a new lightweight chamber made from polyetheretherketone (PEEK). Body weight, burrowing a...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-13924-5 |
| _version_ | 1811224143925870592 |
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| author | Wentao Xie Matthias Lorenz Friederike Poosch Rupert Palme Dietmar Zechner Brigitte Vollmar Eberhard Grambow Daniel Strüder |
| author_facet | Wentao Xie Matthias Lorenz Friederike Poosch Rupert Palme Dietmar Zechner Brigitte Vollmar Eberhard Grambow Daniel Strüder |
| author_sort | Wentao Xie |
| collection | DOAJ |
| description | Abstract The dorsal skinfold chamber is one of the most important in vivo models for repetitive longitudinal assessment of microcirculation and inflammation. This study aimed to refine this model by introducing a new lightweight chamber made from polyetheretherketone (PEEK). Body weight, burrowing activity, distress, faecal corticosterone metabolites and the tilting angle of the chambers were analysed in mice carrying either a standard titanium chamber or a PEEK chamber. Data was obtained before chamber preparation and over a postoperative period of three weeks. In the early postoperative phase, reduced body weight and increased faecal corticosterone metabolites were found in mice with titanium chambers. Chamber tilting and tilting-related complications were reduced in mice with PEEK chambers. The distress score was significantly increased in both groups after chamber preparation, but only returned to preoperative values in mice with PEEK chambers. In summary, we have shown that light chambers reduce animal distress and may extend the maximum dorsal skinfold chamber observation time. Chambers made of PEEK are particularly suitable for this purpose: They are autoclavable, sufficiently stable to withstand rodent bites, inexpensive, and widely available through 3D printing. |
| first_indexed | 2024-04-12T08:45:06Z |
| format | Article |
| id | doaj.art-7c9948c2d6c2470797f5feba7063d7cc |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T08:45:06Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-7c9948c2d6c2470797f5feba7063d7cc2022-12-22T03:39:45ZengNature PortfolioScientific Reports2045-23222022-07-0112111010.1038/s41598-022-13924-53D-printed lightweight dorsal skin fold chambers from PEEK reduce chamber-related animal distressWentao Xie0Matthias Lorenz1Friederike Poosch2Rupert Palme3Dietmar Zechner4Brigitte Vollmar5Eberhard Grambow6Daniel Strüder7Institute for Experimental Surgery, Rostock University Medical CenterFaculty of Engineering, Technology, Business and Design, University of Applied SciencesDepartment of Otorhinolaryngology, Head and Neck Surgery “Otto Koerner”, Rostock University Medical CenterUnit of Physiology, Pathophysiology and Experimental Endocrinology, Department of Biomedical Sciences, University of Veterinary Medicine ViennaInstitute for Experimental Surgery, Rostock University Medical CenterInstitute for Experimental Surgery, Rostock University Medical CenterInstitute for Experimental Surgery, Rostock University Medical CenterInstitute for Experimental Surgery, Rostock University Medical CenterAbstract The dorsal skinfold chamber is one of the most important in vivo models for repetitive longitudinal assessment of microcirculation and inflammation. This study aimed to refine this model by introducing a new lightweight chamber made from polyetheretherketone (PEEK). Body weight, burrowing activity, distress, faecal corticosterone metabolites and the tilting angle of the chambers were analysed in mice carrying either a standard titanium chamber or a PEEK chamber. Data was obtained before chamber preparation and over a postoperative period of three weeks. In the early postoperative phase, reduced body weight and increased faecal corticosterone metabolites were found in mice with titanium chambers. Chamber tilting and tilting-related complications were reduced in mice with PEEK chambers. The distress score was significantly increased in both groups after chamber preparation, but only returned to preoperative values in mice with PEEK chambers. In summary, we have shown that light chambers reduce animal distress and may extend the maximum dorsal skinfold chamber observation time. Chambers made of PEEK are particularly suitable for this purpose: They are autoclavable, sufficiently stable to withstand rodent bites, inexpensive, and widely available through 3D printing.https://doi.org/10.1038/s41598-022-13924-5 |
| spellingShingle | Wentao Xie Matthias Lorenz Friederike Poosch Rupert Palme Dietmar Zechner Brigitte Vollmar Eberhard Grambow Daniel Strüder 3D-printed lightweight dorsal skin fold chambers from PEEK reduce chamber-related animal distress Scientific Reports |
| title | 3D-printed lightweight dorsal skin fold chambers from PEEK reduce chamber-related animal distress |
| title_full | 3D-printed lightweight dorsal skin fold chambers from PEEK reduce chamber-related animal distress |
| title_fullStr | 3D-printed lightweight dorsal skin fold chambers from PEEK reduce chamber-related animal distress |
| title_full_unstemmed | 3D-printed lightweight dorsal skin fold chambers from PEEK reduce chamber-related animal distress |
| title_short | 3D-printed lightweight dorsal skin fold chambers from PEEK reduce chamber-related animal distress |
| title_sort | 3d printed lightweight dorsal skin fold chambers from peek reduce chamber related animal distress |
| url | https://doi.org/10.1038/s41598-022-13924-5 |
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