Wild and Cultivated Species of Rice Have Distinctive Proteomic Responses to Drought
Drought often compromises yield in non-irrigated crops such as rainfed rice, imperiling the communities that depend upon it as a primary food source. In this study, two cultivated species (<i>Oryza sativa</i> cv. Nipponbare and <i>Oryza glaberrima</i> cv. CG14) and an endemic...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-08-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/21/17/5980 |
| Summary: | Drought often compromises yield in non-irrigated crops such as rainfed rice, imperiling the communities that depend upon it as a primary food source. In this study, two cultivated species (<i>Oryza sativa</i> cv. Nipponbare and <i>Oryza glaberrima</i> cv. CG14) and an endemic, perennial Australian wild species (<i>Oryza australiensis</i>) were grown in soil at 40% field capacity for 7 d (drought). The hypothesis was that the natural tolerance of <i>O. australiensis</i> to erratic water supply would be reflected in a unique proteomic profile. Leaves from droughted plants and well-watered controls were harvested for label-free quantitative shotgun proteomics. Physiological and gene ontology analysis confirmed that <i>O. australiensis</i> responded uniquely to drought, with superior leaf water status and enhanced levels of photosynthetic proteins. Distinctive patterns of protein accumulation in drought were observed across the <i>O. australiensis</i> proteome. Photosynthetic and stress-response proteins were more abundant in drought-affected <i>O. glaberrima</i> than <i>O. sativa</i>, and were further enriched in <i>O. australiensis</i>. In contrast, the level of accumulation of photosynthetic proteins decreased when <i>O. sativa</i> underwent drought, while a narrower range of stress-responsive proteins showed increased levels of accumulation. Distinctive proteomic profiles and the accumulated levels of individual proteins with specific functions in response to drought in <i>O. australiensis</i> indicate the importance of this species as a source of stress tolerance genes. |
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| ISSN: | 1661-6596 1422-0067 |