Long-term myofibroblast persistence in the capsular bag contributes to the late spontaneous in-the-bag intraocular lens dislocation
Abstract Late spontaneous in-the-bag intraocular lens (IOL) dislocation is a complication presenting 6 months or later after cataract surgery. We aimed to characterize the cells in the lens capsules (LCs) of 18 patients with spontaneous late in-the-bag IOL dislocation. Patients' average age was...
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Nature Portfolio
2020-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-77207-7 |
| _version_ | 1818681340364587008 |
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| author | Jovana Bisevac Natalia S. Anisimova Richárd Nagymihály Olav Kristianslund Kirankumar Katta Agate Noer Ilias H. Sharafetdinov Liv Drolsum Morten C. Moe Boris E. Malyugin Goran Petrovski |
| author_facet | Jovana Bisevac Natalia S. Anisimova Richárd Nagymihály Olav Kristianslund Kirankumar Katta Agate Noer Ilias H. Sharafetdinov Liv Drolsum Morten C. Moe Boris E. Malyugin Goran Petrovski |
| author_sort | Jovana Bisevac |
| collection | DOAJ |
| description | Abstract Late spontaneous in-the-bag intraocular lens (IOL) dislocation is a complication presenting 6 months or later after cataract surgery. We aimed to characterize the cells in the lens capsules (LCs) of 18 patients with spontaneous late in-the-bag IOL dislocation. Patients' average age was 82.6 ± 1.5 years (range 72–98), and most of them had pseudoexfoliation syndrome (PEX). Cells from the LCs were positive for myofibroblast (αSMA), proliferation (Ki-67, PCNA), early lens development/lens progenitor (SOX2, PAX6), chemokine receptor (CXCR4), and transmembrane (N-cadherin) markers, while negative for epithelial (E-cadherin) marker. Moreover, the cells produced abundant fibronectin, type I and type V collagen in the nearby extracellular matrix (ECM). During ex vivo cultivation of dislocated IOL-LCs in toto, the cells proliferated and likely migrated onto the IOL’s anterior side. EdU proliferation assay confirmed the proliferation potential of the myofibroblasts (MFBs) in dislocated IOL-LCs. Primary cultured lens epithelial cells/MFBs isolated from the LC of dislocated IOLs could induce collagen matrix contraction and continuously proliferated, migrated, and induced ECM remodeling. Taken together, this indicates that long-lived MFBs of dislocated IOLs might contribute to the pathogenic mechanisms in late in-the-bag IOL dislocation. |
| first_indexed | 2024-12-17T10:01:24Z |
| format | Article |
| id | doaj.art-7ca01174659e48f28de3394e74533620 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-17T10:01:24Z |
| publishDate | 2020-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-7ca01174659e48f28de3394e745336202022-12-21T21:53:17ZengNature PortfolioScientific Reports2045-23222020-11-0110111710.1038/s41598-020-77207-7Long-term myofibroblast persistence in the capsular bag contributes to the late spontaneous in-the-bag intraocular lens dislocationJovana Bisevac0Natalia S. Anisimova1Richárd Nagymihály2Olav Kristianslund3Kirankumar Katta4Agate Noer5Ilias H. Sharafetdinov6Liv Drolsum7Morten C. Moe8Boris E. Malyugin9Goran Petrovski10Center for Eye Research, Department of Ophthalmology, University of Oslo and Oslo University HospitalS. Fyodorov Eye Microsurgery Federal State InstitutionCenter for Eye Research, Department of Ophthalmology, University of Oslo and Oslo University HospitalCenter for Eye Research, Department of Ophthalmology, University of Oslo and Oslo University HospitalCenter for Eye Research, Department of Ophthalmology, University of Oslo and Oslo University HospitalCenter for Eye Research, Department of Ophthalmology, University of Oslo and Oslo University HospitalS. Fyodorov Eye Microsurgery Federal State InstitutionCenter for Eye Research, Department of Ophthalmology, University of Oslo and Oslo University HospitalCenter for Eye Research, Department of Ophthalmology, University of Oslo and Oslo University HospitalS. Fyodorov Eye Microsurgery Federal State InstitutionCenter for Eye Research, Department of Ophthalmology, University of Oslo and Oslo University HospitalAbstract Late spontaneous in-the-bag intraocular lens (IOL) dislocation is a complication presenting 6 months or later after cataract surgery. We aimed to characterize the cells in the lens capsules (LCs) of 18 patients with spontaneous late in-the-bag IOL dislocation. Patients' average age was 82.6 ± 1.5 years (range 72–98), and most of them had pseudoexfoliation syndrome (PEX). Cells from the LCs were positive for myofibroblast (αSMA), proliferation (Ki-67, PCNA), early lens development/lens progenitor (SOX2, PAX6), chemokine receptor (CXCR4), and transmembrane (N-cadherin) markers, while negative for epithelial (E-cadherin) marker. Moreover, the cells produced abundant fibronectin, type I and type V collagen in the nearby extracellular matrix (ECM). During ex vivo cultivation of dislocated IOL-LCs in toto, the cells proliferated and likely migrated onto the IOL’s anterior side. EdU proliferation assay confirmed the proliferation potential of the myofibroblasts (MFBs) in dislocated IOL-LCs. Primary cultured lens epithelial cells/MFBs isolated from the LC of dislocated IOLs could induce collagen matrix contraction and continuously proliferated, migrated, and induced ECM remodeling. Taken together, this indicates that long-lived MFBs of dislocated IOLs might contribute to the pathogenic mechanisms in late in-the-bag IOL dislocation.https://doi.org/10.1038/s41598-020-77207-7 |
| spellingShingle | Jovana Bisevac Natalia S. Anisimova Richárd Nagymihály Olav Kristianslund Kirankumar Katta Agate Noer Ilias H. Sharafetdinov Liv Drolsum Morten C. Moe Boris E. Malyugin Goran Petrovski Long-term myofibroblast persistence in the capsular bag contributes to the late spontaneous in-the-bag intraocular lens dislocation Scientific Reports |
| title | Long-term myofibroblast persistence in the capsular bag contributes to the late spontaneous in-the-bag intraocular lens dislocation |
| title_full | Long-term myofibroblast persistence in the capsular bag contributes to the late spontaneous in-the-bag intraocular lens dislocation |
| title_fullStr | Long-term myofibroblast persistence in the capsular bag contributes to the late spontaneous in-the-bag intraocular lens dislocation |
| title_full_unstemmed | Long-term myofibroblast persistence in the capsular bag contributes to the late spontaneous in-the-bag intraocular lens dislocation |
| title_short | Long-term myofibroblast persistence in the capsular bag contributes to the late spontaneous in-the-bag intraocular lens dislocation |
| title_sort | long term myofibroblast persistence in the capsular bag contributes to the late spontaneous in the bag intraocular lens dislocation |
| url | https://doi.org/10.1038/s41598-020-77207-7 |
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