Identification of circulating microRNAs for the differential diagnosis of Parkinson’s disease and Multiple System Atrophy.
Background. Parkinson's disease (PD) is a progressive neurodegenerative disorder which may be misdiagnosed with atypical conditions such as Multiple System Atrophy (MSA), due to overlapping clinical features. MicroRNAs (miRNAs) are small noncoding RNAs with a key role in post-transcriptional ge...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2014-06-01
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| Series: | Frontiers in Cellular Neuroscience |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00156/full |
| _version_ | 1818134844009349120 |
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| author | Annamaria eVallelunga Marco eRagusa Stefania eDi Mauro Tommaso eIannitti Manuela ePilleri Roberta eBiundo Luca eWeis Cinzia eDi Pietro Angela eDe Iuliis Alessandra eNicoletti Mario eZappia Michele ePurrello Angelo eAntonini |
| author_facet | Annamaria eVallelunga Marco eRagusa Stefania eDi Mauro Tommaso eIannitti Manuela ePilleri Roberta eBiundo Luca eWeis Cinzia eDi Pietro Angela eDe Iuliis Alessandra eNicoletti Mario eZappia Michele ePurrello Angelo eAntonini |
| author_sort | Annamaria eVallelunga |
| collection | DOAJ |
| description | Background. Parkinson's disease (PD) is a progressive neurodegenerative disorder which may be misdiagnosed with atypical conditions such as Multiple System Atrophy (MSA), due to overlapping clinical features. MicroRNAs (miRNAs) are small noncoding RNAs with a key role in post-transcriptional gene regulation. We hypothesized that identification of a distinct set of circulating miRNAs (cmiRNAs) could distinguish patients affected by PD from MSA and healthy individuals. Results. Using TaqMan Low Density Array technology, we analysed 754 miRNAs and found 9 cmiRNAs differentially expressed in PD and MSA patients compared to healthy controls. We also validated a set of 4 differentially expressed cmiRNAs in PD and MSA patients versus controls. More specifically, miR-339-5p was downregulated, whereas miR-223*, miR-324-3p and mir-24 were upregulated in both diseases. We found cmiRNAs specifically deregulated in PD (downregulation of miR-30c and miR-148b) and in MSA (upregulation of miR-148b). Finally, comparing MSA and PD, we identified 3 upregulated cmiRNAs in MSA serum (miR-24, miR-34b, miR-148b). Conclusions. Our results suggest that cmiRNA signatures discriminate PD from MSA patients and healthy controls and may be considered specific, non-invasive biomarkers for differential diagnosis. |
| first_indexed | 2024-12-11T09:15:04Z |
| format | Article |
| id | doaj.art-7ca1f714c23540b299674f20fbc74318 |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-11T09:15:04Z |
| publishDate | 2014-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-7ca1f714c23540b299674f20fbc743182022-12-22T01:13:24ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022014-06-01810.3389/fncel.2014.0015696340Identification of circulating microRNAs for the differential diagnosis of Parkinson’s disease and Multiple System Atrophy.Annamaria eVallelunga0Marco eRagusa1Stefania eDi Mauro2Tommaso eIannitti3Manuela ePilleri4Roberta eBiundo5Luca eWeis6Cinzia eDi Pietro7Angela eDe Iuliis8Alessandra eNicoletti9Mario eZappia10Michele ePurrello11Angelo eAntonini12IRCCS Hospital San CamilloUniversity of CataniaUniversity of CataniaUniversity of LeedsIRCCS Hospital San CamilloIRCCS Hospital San CamilloIRCCS Hospital San CamilloUniversity of CataniaUniversity of PaduaUniversity of CataniaUniversity of CataniaUniversity of CataniaIRCCS Hospital San CamilloBackground. Parkinson's disease (PD) is a progressive neurodegenerative disorder which may be misdiagnosed with atypical conditions such as Multiple System Atrophy (MSA), due to overlapping clinical features. MicroRNAs (miRNAs) are small noncoding RNAs with a key role in post-transcriptional gene regulation. We hypothesized that identification of a distinct set of circulating miRNAs (cmiRNAs) could distinguish patients affected by PD from MSA and healthy individuals. Results. Using TaqMan Low Density Array technology, we analysed 754 miRNAs and found 9 cmiRNAs differentially expressed in PD and MSA patients compared to healthy controls. We also validated a set of 4 differentially expressed cmiRNAs in PD and MSA patients versus controls. More specifically, miR-339-5p was downregulated, whereas miR-223*, miR-324-3p and mir-24 were upregulated in both diseases. We found cmiRNAs specifically deregulated in PD (downregulation of miR-30c and miR-148b) and in MSA (upregulation of miR-148b). Finally, comparing MSA and PD, we identified 3 upregulated cmiRNAs in MSA serum (miR-24, miR-34b, miR-148b). Conclusions. Our results suggest that cmiRNA signatures discriminate PD from MSA patients and healthy controls and may be considered specific, non-invasive biomarkers for differential diagnosis.http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00156/fullMicroRNAsMultiple System AtrophyParkinson’s diseaseearly diagnosiscirculating microRNAsAtypical Parkinsonian Disorders |
| spellingShingle | Annamaria eVallelunga Marco eRagusa Stefania eDi Mauro Tommaso eIannitti Manuela ePilleri Roberta eBiundo Luca eWeis Cinzia eDi Pietro Angela eDe Iuliis Alessandra eNicoletti Mario eZappia Michele ePurrello Angelo eAntonini Identification of circulating microRNAs for the differential diagnosis of Parkinson’s disease and Multiple System Atrophy. Frontiers in Cellular Neuroscience MicroRNAs Multiple System Atrophy Parkinson’s disease early diagnosis circulating microRNAs Atypical Parkinsonian Disorders |
| title | Identification of circulating microRNAs for the differential diagnosis of Parkinson’s disease and Multiple System Atrophy. |
| title_full | Identification of circulating microRNAs for the differential diagnosis of Parkinson’s disease and Multiple System Atrophy. |
| title_fullStr | Identification of circulating microRNAs for the differential diagnosis of Parkinson’s disease and Multiple System Atrophy. |
| title_full_unstemmed | Identification of circulating microRNAs for the differential diagnosis of Parkinson’s disease and Multiple System Atrophy. |
| title_short | Identification of circulating microRNAs for the differential diagnosis of Parkinson’s disease and Multiple System Atrophy. |
| title_sort | identification of circulating micrornas for the differential diagnosis of parkinson s disease and multiple system atrophy |
| topic | MicroRNAs Multiple System Atrophy Parkinson’s disease early diagnosis circulating microRNAs Atypical Parkinsonian Disorders |
| url | http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00156/full |
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