Characterization of low-density granulocytes in COVID-19.
Severe COVID-19 is characterized by extensive pulmonary complications, to which host immune responses are believed to play a role. As the major arm of innate immunity, neutrophils are one of the first cells recruited to the site of infection where their excessive activation can contribute to lung pa...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-07-01
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| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1009721 |
| _version_ | 1818716633334546432 |
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| author | Luz E Cabrera Pirkka T Pekkarinen Maria Alander Kirsten H A Nowlan Ngoc Anh Nguyen Suvi Jokiranta Suvi Kuivanen Anu Patjas Sointu Mero Sari H Pakkanen Santtu Heinonen Anu Kantele Olli Vapalahti Eliisa Kekäläinen Tomas Strandin |
| author_facet | Luz E Cabrera Pirkka T Pekkarinen Maria Alander Kirsten H A Nowlan Ngoc Anh Nguyen Suvi Jokiranta Suvi Kuivanen Anu Patjas Sointu Mero Sari H Pakkanen Santtu Heinonen Anu Kantele Olli Vapalahti Eliisa Kekäläinen Tomas Strandin |
| author_sort | Luz E Cabrera |
| collection | DOAJ |
| description | Severe COVID-19 is characterized by extensive pulmonary complications, to which host immune responses are believed to play a role. As the major arm of innate immunity, neutrophils are one of the first cells recruited to the site of infection where their excessive activation can contribute to lung pathology. Low-density granulocytes (LDGs) are circulating neutrophils, whose numbers increase in some autoimmune diseases and cancer, but are poorly characterized in acute viral infections. Using flow cytometry, we detected a significant increase of LDGs in the blood of acute COVID-19 patients, compared to healthy controls. Based on their surface marker expression, COVID-19-related LDGs exhibit four different populations, which display distinctive stages of granulocytic development and most likely reflect emergency myelopoiesis. Moreover, COVID-19 LDGs show a link with an elevated recruitment and activation of neutrophils. Functional assays demonstrated the immunosuppressive capacities of these cells, which might contribute to impaired lymphocyte responses during acute disease. Taken together, our data confirms a significant granulocyte activation during COVID-19 and suggests that granulocytes of lower density play a role in disease progression. |
| first_indexed | 2024-12-17T19:22:22Z |
| format | Article |
| id | doaj.art-7ca21f621ae944f98c160bcd62477a02 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-17T19:22:22Z |
| publishDate | 2021-07-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-7ca21f621ae944f98c160bcd62477a022022-12-21T21:35:28ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742021-07-01177e100972110.1371/journal.ppat.1009721Characterization of low-density granulocytes in COVID-19.Luz E CabreraPirkka T PekkarinenMaria AlanderKirsten H A NowlanNgoc Anh NguyenSuvi JokirantaSuvi KuivanenAnu PatjasSointu MeroSari H PakkanenSanttu HeinonenAnu KanteleOlli VapalahtiEliisa KekäläinenTomas StrandinSevere COVID-19 is characterized by extensive pulmonary complications, to which host immune responses are believed to play a role. As the major arm of innate immunity, neutrophils are one of the first cells recruited to the site of infection where their excessive activation can contribute to lung pathology. Low-density granulocytes (LDGs) are circulating neutrophils, whose numbers increase in some autoimmune diseases and cancer, but are poorly characterized in acute viral infections. Using flow cytometry, we detected a significant increase of LDGs in the blood of acute COVID-19 patients, compared to healthy controls. Based on their surface marker expression, COVID-19-related LDGs exhibit four different populations, which display distinctive stages of granulocytic development and most likely reflect emergency myelopoiesis. Moreover, COVID-19 LDGs show a link with an elevated recruitment and activation of neutrophils. Functional assays demonstrated the immunosuppressive capacities of these cells, which might contribute to impaired lymphocyte responses during acute disease. Taken together, our data confirms a significant granulocyte activation during COVID-19 and suggests that granulocytes of lower density play a role in disease progression.https://doi.org/10.1371/journal.ppat.1009721 |
| spellingShingle | Luz E Cabrera Pirkka T Pekkarinen Maria Alander Kirsten H A Nowlan Ngoc Anh Nguyen Suvi Jokiranta Suvi Kuivanen Anu Patjas Sointu Mero Sari H Pakkanen Santtu Heinonen Anu Kantele Olli Vapalahti Eliisa Kekäläinen Tomas Strandin Characterization of low-density granulocytes in COVID-19. PLoS Pathogens |
| title | Characterization of low-density granulocytes in COVID-19. |
| title_full | Characterization of low-density granulocytes in COVID-19. |
| title_fullStr | Characterization of low-density granulocytes in COVID-19. |
| title_full_unstemmed | Characterization of low-density granulocytes in COVID-19. |
| title_short | Characterization of low-density granulocytes in COVID-19. |
| title_sort | characterization of low density granulocytes in covid 19 |
| url | https://doi.org/10.1371/journal.ppat.1009721 |
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