Yeast GH30 Xylanase from <em>Sugiyamaella lignohabitans</em> Is a Glucuronoxylanase with Auxiliary Xylobiohydrolase Activity
Xylanases are the enzymes that catalyze the breakdown of the main hemicellulose present in plant cell walls. They have attracted attention due to their biotechnological potential for the preparation of industrially interesting products from lignocellulose. While many xylanases have been characterize...
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MDPI AG
2022-01-01
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| Loạt: | Molecules |
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| Truy cập trực tuyến: | https://www.mdpi.com/1420-3049/27/3/751 |
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| author | Katarína Šuchová Andrej Chyba Zuzana Hegyi Martin Rebroš Vladimír Puchart |
| author_facet | Katarína Šuchová Andrej Chyba Zuzana Hegyi Martin Rebroš Vladimír Puchart |
| author_sort | Katarína Šuchová |
| collection | DOAJ |
| description | Xylanases are the enzymes that catalyze the breakdown of the main hemicellulose present in plant cell walls. They have attracted attention due to their biotechnological potential for the preparation of industrially interesting products from lignocellulose. While many xylanases have been characterized from bacteria and filamentous fungi, information on yeast xylanases is scarce and no yeast xylanase belonging to glycoside hydrolase (GH) family 30 has been described so far. Here, we cloned, expressed and characterized GH30 xylanase <i>Sl</i>Xyn30A from the yeast <i>Sugiyamaella lignohabitans</i>. The enzyme is active on glucuronoxylan (8.4 U/mg) and rhodymenan (linear β-1,4-1,3-xylan) (3.1 U/mg) while its activity on arabinoxylan is very low (0.03 U/mg). From glucuronoxylan <i>Sl</i>Xyn30A releases a series of acidic xylooligosaccharides of general formula MeGlcA<sup>2</sup>Xyl<sub>n</sub>. These products, which are typical for GH30-specific glucuronoxylanases, are subsequently shortened at the non-reducing end, from which xylobiose moieties are liberated. Xylobiohydrolase activity was also observed during the hydrolysis of various xylooligosaccharides. <i>Sl</i>Xyn30A thus expands the group of glucuronoxylanases/xylobiohydrolases which has been hitherto represented only by several fungal GH30-7 members. |
| first_indexed | 2024-03-09T23:29:18Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T23:29:18Z |
| publishDate | 2022-01-01 |
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| series | Molecules |
| spelling | doaj.art-7ca83b2a2ae04d18bc8ed1cae1b8cc692023-11-23T17:12:07ZengMDPI AGMolecules1420-30492022-01-0127375110.3390/molecules27030751Yeast GH30 Xylanase from <em>Sugiyamaella lignohabitans</em> Is a Glucuronoxylanase with Auxiliary Xylobiohydrolase ActivityKatarína Šuchová0Andrej Chyba1Zuzana Hegyi2Martin Rebroš3Vladimír Puchart4Institute of Chemistry, Slovak Academy of Sciences, Dúbravská Cesta 9, 845 38 Bratislava, SlovakiaInstitute of Chemistry, Slovak Academy of Sciences, Dúbravská Cesta 9, 845 38 Bratislava, SlovakiaInstitute of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37 Bratislava, SlovakiaInstitute of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37 Bratislava, SlovakiaInstitute of Chemistry, Slovak Academy of Sciences, Dúbravská Cesta 9, 845 38 Bratislava, SlovakiaXylanases are the enzymes that catalyze the breakdown of the main hemicellulose present in plant cell walls. They have attracted attention due to their biotechnological potential for the preparation of industrially interesting products from lignocellulose. While many xylanases have been characterized from bacteria and filamentous fungi, information on yeast xylanases is scarce and no yeast xylanase belonging to glycoside hydrolase (GH) family 30 has been described so far. Here, we cloned, expressed and characterized GH30 xylanase <i>Sl</i>Xyn30A from the yeast <i>Sugiyamaella lignohabitans</i>. The enzyme is active on glucuronoxylan (8.4 U/mg) and rhodymenan (linear β-1,4-1,3-xylan) (3.1 U/mg) while its activity on arabinoxylan is very low (0.03 U/mg). From glucuronoxylan <i>Sl</i>Xyn30A releases a series of acidic xylooligosaccharides of general formula MeGlcA<sup>2</sup>Xyl<sub>n</sub>. These products, which are typical for GH30-specific glucuronoxylanases, are subsequently shortened at the non-reducing end, from which xylobiose moieties are liberated. Xylobiohydrolase activity was also observed during the hydrolysis of various xylooligosaccharides. <i>Sl</i>Xyn30A thus expands the group of glucuronoxylanases/xylobiohydrolases which has been hitherto represented only by several fungal GH30-7 members.https://www.mdpi.com/1420-3049/27/3/751xylanaseglucuronoxylanasexylobiohydrolasexylanglycoside hydrolase family 30GH30-7 subfamily |
| spellingShingle | Katarína Šuchová Andrej Chyba Zuzana Hegyi Martin Rebroš Vladimír Puchart Yeast GH30 Xylanase from <em>Sugiyamaella lignohabitans</em> Is a Glucuronoxylanase with Auxiliary Xylobiohydrolase Activity Molecules xylanase glucuronoxylanase xylobiohydrolase xylan glycoside hydrolase family 30 GH30-7 subfamily |
| title | Yeast GH30 Xylanase from <em>Sugiyamaella lignohabitans</em> Is a Glucuronoxylanase with Auxiliary Xylobiohydrolase Activity |
| title_full | Yeast GH30 Xylanase from <em>Sugiyamaella lignohabitans</em> Is a Glucuronoxylanase with Auxiliary Xylobiohydrolase Activity |
| title_fullStr | Yeast GH30 Xylanase from <em>Sugiyamaella lignohabitans</em> Is a Glucuronoxylanase with Auxiliary Xylobiohydrolase Activity |
| title_full_unstemmed | Yeast GH30 Xylanase from <em>Sugiyamaella lignohabitans</em> Is a Glucuronoxylanase with Auxiliary Xylobiohydrolase Activity |
| title_short | Yeast GH30 Xylanase from <em>Sugiyamaella lignohabitans</em> Is a Glucuronoxylanase with Auxiliary Xylobiohydrolase Activity |
| title_sort | yeast gh30 xylanase from em sugiyamaella lignohabitans em is a glucuronoxylanase with auxiliary xylobiohydrolase activity |
| topic | xylanase glucuronoxylanase xylobiohydrolase xylan glycoside hydrolase family 30 GH30-7 subfamily |
| url | https://www.mdpi.com/1420-3049/27/3/751 |
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