Vector potential dual effect of promoting the proliferation of chondrocytes and inhibiting the calcification process in the articular cartilage
Abstract OA commonly affects the articular cartilage of the tibia, and its calcification worsens its advancement and its prevalence has recently increased. Vector potential (VP) represents a novel physical therapy for treating OA. Since the impact of VP on articular cartilage remains unknown, we aim...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-43949-3 |
| _version_ | 1827634187338776576 |
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| author | Hirai Suito Wataru Minamizono Nao Yashima Hiroya Matsunaga Kaoru Fujikawa Masafumi Ohsako |
| author_facet | Hirai Suito Wataru Minamizono Nao Yashima Hiroya Matsunaga Kaoru Fujikawa Masafumi Ohsako |
| author_sort | Hirai Suito |
| collection | DOAJ |
| description | Abstract OA commonly affects the articular cartilage of the tibia, and its calcification worsens its advancement and its prevalence has recently increased. Vector potential (VP) represents a novel physical therapy for treating OA. Since the impact of VP on articular cartilage remains unknown, we aimed to assess its effects on articular cartilage and its potential as a new treatment for OA. Here, we divided 24 male Wistar rats, 6-week-old, into control (CO, n = 12) and VP stimulus (n = 12) groups (VP conditions: volt, 67 mV; frequency, 20 kHz; current, 0.12 mA; experimental frequency, 30 min/days, 5 days/week, and 3 weeks). Articular cartilage can be classified into four layers: superficial, medial, deep, and calcified. Moreover, the number of chondrocytes in the articular cartilage was higher in the CO group compared to the VP group, although the calcified layer was thinner in the VP group. Furthermore, MKi67 exhibited higher expression in the VP group than in the CO group, while ectonucleotide pyrophosphatase/phosphodiesterase 1 was downregulated in the VP group. Our findings indicate that VP positively influenced chondrocyte proliferation and inhibited calcification in articular cartilage. Thus, VP stimulation may assist in the development of novel strategies for preventing OA. |
| first_indexed | 2024-03-09T15:10:52Z |
| format | Article |
| id | doaj.art-7ca8a0ef814f4c78ab6cdb02bdf1219d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:10:52Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-7ca8a0ef814f4c78ab6cdb02bdf1219d2023-11-26T13:24:17ZengNature PortfolioScientific Reports2045-23222023-10-0113111210.1038/s41598-023-43949-3Vector potential dual effect of promoting the proliferation of chondrocytes and inhibiting the calcification process in the articular cartilageHirai Suito0Wataru Minamizono1Nao Yashima2Hiroya Matsunaga3Kaoru Fujikawa4Masafumi Ohsako5Graduate School of Human Life Design, Toyo UniversityGraduate School of Human Life Design, Toyo UniversityGraduate School of Health Sports Science, Toyo UniversityGraduate School of Health Sports Science, Toyo UniversityDepartment of Oral Anatomy and Developmental Biology, Showa University School of DensityGraduate School of Health Sports Science, Toyo UniversityAbstract OA commonly affects the articular cartilage of the tibia, and its calcification worsens its advancement and its prevalence has recently increased. Vector potential (VP) represents a novel physical therapy for treating OA. Since the impact of VP on articular cartilage remains unknown, we aimed to assess its effects on articular cartilage and its potential as a new treatment for OA. Here, we divided 24 male Wistar rats, 6-week-old, into control (CO, n = 12) and VP stimulus (n = 12) groups (VP conditions: volt, 67 mV; frequency, 20 kHz; current, 0.12 mA; experimental frequency, 30 min/days, 5 days/week, and 3 weeks). Articular cartilage can be classified into four layers: superficial, medial, deep, and calcified. Moreover, the number of chondrocytes in the articular cartilage was higher in the CO group compared to the VP group, although the calcified layer was thinner in the VP group. Furthermore, MKi67 exhibited higher expression in the VP group than in the CO group, while ectonucleotide pyrophosphatase/phosphodiesterase 1 was downregulated in the VP group. Our findings indicate that VP positively influenced chondrocyte proliferation and inhibited calcification in articular cartilage. Thus, VP stimulation may assist in the development of novel strategies for preventing OA.https://doi.org/10.1038/s41598-023-43949-3 |
| spellingShingle | Hirai Suito Wataru Minamizono Nao Yashima Hiroya Matsunaga Kaoru Fujikawa Masafumi Ohsako Vector potential dual effect of promoting the proliferation of chondrocytes and inhibiting the calcification process in the articular cartilage Scientific Reports |
| title | Vector potential dual effect of promoting the proliferation of chondrocytes and inhibiting the calcification process in the articular cartilage |
| title_full | Vector potential dual effect of promoting the proliferation of chondrocytes and inhibiting the calcification process in the articular cartilage |
| title_fullStr | Vector potential dual effect of promoting the proliferation of chondrocytes and inhibiting the calcification process in the articular cartilage |
| title_full_unstemmed | Vector potential dual effect of promoting the proliferation of chondrocytes and inhibiting the calcification process in the articular cartilage |
| title_short | Vector potential dual effect of promoting the proliferation of chondrocytes and inhibiting the calcification process in the articular cartilage |
| title_sort | vector potential dual effect of promoting the proliferation of chondrocytes and inhibiting the calcification process in the articular cartilage |
| url | https://doi.org/10.1038/s41598-023-43949-3 |
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