Transmitted drug resistance and transmission clusters among HIV-1 treatment-naïve patients in Guangdong, China: a cross-sectional study
Abstract Background Transmitted drug resistance (TDR) that affects the effectiveness of the first-line antiretroviral therapy (ART) regimen is becoming prevalent worldwide. However, its prevalence and transmission among HIV-1 treatment-naïve patients in Guangdong, China are rarely reported. We aimed...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-09-01
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| Series: | Virology Journal |
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| Online Access: | https://doi.org/10.1186/s12985-021-01653-6 |
| _version_ | 1818728688759341056 |
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| author | Yun Lan Linghua Li Xiang He Fengyu Hu Xizi Deng Weiping Cai Junbin Li Xuemei Ling Qinghong Fan Xiaoli Cai Liya Li Feng Li Xiaoping Tang |
| author_facet | Yun Lan Linghua Li Xiang He Fengyu Hu Xizi Deng Weiping Cai Junbin Li Xuemei Ling Qinghong Fan Xiaoli Cai Liya Li Feng Li Xiaoping Tang |
| author_sort | Yun Lan |
| collection | DOAJ |
| description | Abstract Background Transmitted drug resistance (TDR) that affects the effectiveness of the first-line antiretroviral therapy (ART) regimen is becoming prevalent worldwide. However, its prevalence and transmission among HIV-1 treatment-naïve patients in Guangdong, China are rarely reported. We aimed to comprehensively analyze the prevalence of TDR and the transmission clusters of HIV-1 infected persons before ART in Guangdong. Methods The HIV-1 treatment-naïve patients were recruited between January 2018 and December 2018. The HIV-1 pol region was amplified by reverse transcriptional PCR and sequenced by sanger sequencing. Genotypes, surveillance drug resistance mutations (SDRMs) and TDR were analyzed. Genetic transmission clusters among patients were identified by pairwise Tamura-Nei 93 genetic distance, with a threshold of 0.015. Results A total of 2368 (97.17%) HIV-1 pol sequences were successfully amplified and sequenced from the enrolled 2437 patients. CRF07_BC (35.90%, 850/2368), CRF01_AE (35.56%, 842/2368) and CRF55_01B (10.30%, 244/2368) were the main HIV-1 genotypes circulating in Guangdong. Twenty-one SDRMs were identified among fifty-two drug-resistant sequences. The overall prevalence of TDR was 2.20% (52/2368). Among the 2368 patients who underwent sequencing, 8 (0.34%) had TDR to protease inhibitors (PIs), 22 (0.93%) to nucleoside reverse transcriptase inhibitors (NRTIs), and 23 (0.97%) to non-nucleoside reverse transcriptase inhibitors (NNRTIs). Two (0.08%) sequences showed dual-class resistance to both NRTIs and NNRTIs, and no sequences showed triple-class resistance. A total of 1066 (45.02%) sequences were segregated into 194 clusters, ranging from 2 to 414 sequences. In total, 15 (28.85%) of patients with TDR were included in 9 clusters; one cluster contained two TDR sequences with the K103N mutation was observed. Conclusions There is high HIV-1 genetic heterogeneity among patients in Guangdong. Although the overall prevalence of TDR is low, it is still necessary to remain vigilant regarding some important SDRMs. |
| first_indexed | 2024-12-17T22:33:58Z |
| format | Article |
| id | doaj.art-7ca9edfe90d44dcd96caa5230901ddcc |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-17T22:33:58Z |
| publishDate | 2021-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-7ca9edfe90d44dcd96caa5230901ddcc2022-12-21T21:30:08ZengBMCVirology Journal1743-422X2021-09-011811910.1186/s12985-021-01653-6Transmitted drug resistance and transmission clusters among HIV-1 treatment-naïve patients in Guangdong, China: a cross-sectional studyYun Lan0Linghua Li1Xiang He2Fengyu Hu3Xizi Deng4Weiping Cai5Junbin Li6Xuemei Ling7Qinghong Fan8Xiaoli Cai9Liya Li10Feng Li11Xiaoping Tang12Guangzhou Eighth People’s Hospital, Guangzhou Medical UniversityGuangzhou Eighth People’s Hospital, Guangzhou Medical UniversityGuangdong Provincial Institute of Public Health, Guangdong Provincial Center for Disease Control and PreventionGuangzhou Eighth People’s Hospital, Guangzhou Medical UniversityGuangzhou Eighth People’s Hospital, Guangzhou Medical UniversityGuangzhou Eighth People’s Hospital, Guangzhou Medical UniversityGuangdong Center for Diagnosis and Treatment of AIDSGuangzhou Eighth People’s Hospital, Guangzhou Medical UniversityGuangzhou Eighth People’s Hospital, Guangzhou Medical UniversityGuangzhou Eighth People’s Hospital, Guangzhou Medical UniversityGuangzhou Eighth People’s Hospital, Guangzhou Medical UniversityGuangzhou Eighth People’s Hospital, Guangzhou Medical UniversityGuangzhou Eighth People’s Hospital, Guangzhou Medical UniversityAbstract Background Transmitted drug resistance (TDR) that affects the effectiveness of the first-line antiretroviral therapy (ART) regimen is becoming prevalent worldwide. However, its prevalence and transmission among HIV-1 treatment-naïve patients in Guangdong, China are rarely reported. We aimed to comprehensively analyze the prevalence of TDR and the transmission clusters of HIV-1 infected persons before ART in Guangdong. Methods The HIV-1 treatment-naïve patients were recruited between January 2018 and December 2018. The HIV-1 pol region was amplified by reverse transcriptional PCR and sequenced by sanger sequencing. Genotypes, surveillance drug resistance mutations (SDRMs) and TDR were analyzed. Genetic transmission clusters among patients were identified by pairwise Tamura-Nei 93 genetic distance, with a threshold of 0.015. Results A total of 2368 (97.17%) HIV-1 pol sequences were successfully amplified and sequenced from the enrolled 2437 patients. CRF07_BC (35.90%, 850/2368), CRF01_AE (35.56%, 842/2368) and CRF55_01B (10.30%, 244/2368) were the main HIV-1 genotypes circulating in Guangdong. Twenty-one SDRMs were identified among fifty-two drug-resistant sequences. The overall prevalence of TDR was 2.20% (52/2368). Among the 2368 patients who underwent sequencing, 8 (0.34%) had TDR to protease inhibitors (PIs), 22 (0.93%) to nucleoside reverse transcriptase inhibitors (NRTIs), and 23 (0.97%) to non-nucleoside reverse transcriptase inhibitors (NNRTIs). Two (0.08%) sequences showed dual-class resistance to both NRTIs and NNRTIs, and no sequences showed triple-class resistance. A total of 1066 (45.02%) sequences were segregated into 194 clusters, ranging from 2 to 414 sequences. In total, 15 (28.85%) of patients with TDR were included in 9 clusters; one cluster contained two TDR sequences with the K103N mutation was observed. Conclusions There is high HIV-1 genetic heterogeneity among patients in Guangdong. Although the overall prevalence of TDR is low, it is still necessary to remain vigilant regarding some important SDRMs.https://doi.org/10.1186/s12985-021-01653-6HIV-1Transmitted drug resistanceTransmission clusterGuangdong |
| spellingShingle | Yun Lan Linghua Li Xiang He Fengyu Hu Xizi Deng Weiping Cai Junbin Li Xuemei Ling Qinghong Fan Xiaoli Cai Liya Li Feng Li Xiaoping Tang Transmitted drug resistance and transmission clusters among HIV-1 treatment-naïve patients in Guangdong, China: a cross-sectional study Virology Journal HIV-1 Transmitted drug resistance Transmission cluster Guangdong |
| title | Transmitted drug resistance and transmission clusters among HIV-1 treatment-naïve patients in Guangdong, China: a cross-sectional study |
| title_full | Transmitted drug resistance and transmission clusters among HIV-1 treatment-naïve patients in Guangdong, China: a cross-sectional study |
| title_fullStr | Transmitted drug resistance and transmission clusters among HIV-1 treatment-naïve patients in Guangdong, China: a cross-sectional study |
| title_full_unstemmed | Transmitted drug resistance and transmission clusters among HIV-1 treatment-naïve patients in Guangdong, China: a cross-sectional study |
| title_short | Transmitted drug resistance and transmission clusters among HIV-1 treatment-naïve patients in Guangdong, China: a cross-sectional study |
| title_sort | transmitted drug resistance and transmission clusters among hiv 1 treatment naive patients in guangdong china a cross sectional study |
| topic | HIV-1 Transmitted drug resistance Transmission cluster Guangdong |
| url | https://doi.org/10.1186/s12985-021-01653-6 |
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