Viral Infection at High Magnification: 3D Electron Microscopy Methods to Analyze the Architecture of Infected Cells
As obligate intracellular parasites, viruses need to hijack their cellular hosts and reprogram their machineries in order to replicate their genomes and produce new virions. For the direct visualization of the different steps of a viral life cycle (attachment, entry, replication, assembly and egress...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-12-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/7/12/2940 |
| _version_ | 1818246426933592064 |
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| author | Inés Romero-Brey Ralf Bartenschlager |
| author_facet | Inés Romero-Brey Ralf Bartenschlager |
| author_sort | Inés Romero-Brey |
| collection | DOAJ |
| description | As obligate intracellular parasites, viruses need to hijack their cellular hosts and reprogram their machineries in order to replicate their genomes and produce new virions. For the direct visualization of the different steps of a viral life cycle (attachment, entry, replication, assembly and egress) electron microscopy (EM) methods are extremely helpful. While conventional EM has given important information about virus-host cell interactions, the development of three-dimensional EM (3D-EM) approaches provides unprecedented insights into how viruses remodel the intracellular architecture of the host cell. During the last years several 3D-EM methods have been developed. Here we will provide a description of the main approaches and examples of innovative applications. |
| first_indexed | 2024-12-12T14:48:38Z |
| format | Article |
| id | doaj.art-7cae383b0ff742a597eb9f5dd047eb8b |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-12T14:48:38Z |
| publishDate | 2015-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-7cae383b0ff742a597eb9f5dd047eb8b2022-12-22T00:21:04ZengMDPI AGViruses1999-49152015-12-017126316634510.3390/v7122940v7122940Viral Infection at High Magnification: 3D Electron Microscopy Methods to Analyze the Architecture of Infected CellsInés Romero-Brey0Ralf Bartenschlager1Department of Infectious Diseases, Molecular Virology, Heidelberg University, Im Neuenheimer Feld 345, 69120 Heidelberg, GermanyDepartment of Infectious Diseases, Molecular Virology, Heidelberg University, Im Neuenheimer Feld 345, 69120 Heidelberg, GermanyAs obligate intracellular parasites, viruses need to hijack their cellular hosts and reprogram their machineries in order to replicate their genomes and produce new virions. For the direct visualization of the different steps of a viral life cycle (attachment, entry, replication, assembly and egress) electron microscopy (EM) methods are extremely helpful. While conventional EM has given important information about virus-host cell interactions, the development of three-dimensional EM (3D-EM) approaches provides unprecedented insights into how viruses remodel the intracellular architecture of the host cell. During the last years several 3D-EM methods have been developed. Here we will provide a description of the main approaches and examples of innovative applications.http://www.mdpi.com/1999-4915/7/12/2940transmission electron microscopyelectron tomographyserial sectioningscanning transmission electron microscopyserial block face-scanning electron microscopyfocus ion beam-scanning electron microscopyvirus-host interactionscell membranesultrastructurecryo-EM |
| spellingShingle | Inés Romero-Brey Ralf Bartenschlager Viral Infection at High Magnification: 3D Electron Microscopy Methods to Analyze the Architecture of Infected Cells Viruses transmission electron microscopy electron tomography serial sectioning scanning transmission electron microscopy serial block face-scanning electron microscopy focus ion beam-scanning electron microscopy virus-host interactions cell membranes ultrastructure cryo-EM |
| title | Viral Infection at High Magnification: 3D Electron Microscopy Methods to Analyze the Architecture of Infected Cells |
| title_full | Viral Infection at High Magnification: 3D Electron Microscopy Methods to Analyze the Architecture of Infected Cells |
| title_fullStr | Viral Infection at High Magnification: 3D Electron Microscopy Methods to Analyze the Architecture of Infected Cells |
| title_full_unstemmed | Viral Infection at High Magnification: 3D Electron Microscopy Methods to Analyze the Architecture of Infected Cells |
| title_short | Viral Infection at High Magnification: 3D Electron Microscopy Methods to Analyze the Architecture of Infected Cells |
| title_sort | viral infection at high magnification 3d electron microscopy methods to analyze the architecture of infected cells |
| topic | transmission electron microscopy electron tomography serial sectioning scanning transmission electron microscopy serial block face-scanning electron microscopy focus ion beam-scanning electron microscopy virus-host interactions cell membranes ultrastructure cryo-EM |
| url | http://www.mdpi.com/1999-4915/7/12/2940 |
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