Scaffold based on castor oil as an osteoconductive matrix in bone repair: biocompatibility analysis
Abstract To analyze the biocompatibility of the scaffold produced from a natural polymer derived from castor oil through hemolytic activity and antimicrobial activity, to enable the clinical application. Three in vitro tests were performed: Hemolytic activity test - Polymer partially dissolved in co...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Polímeros
2022-01-01
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| Series: | Polímeros |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282022000100403&tlng=en |
| _version_ | 1818329593823625216 |
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| author | Fabianne Soares Lima Luis Felipe Matos Isnayra Kerolaynne Pacheco Fernando Reis João Victor Frazão Câmara Josué Junior Araujo Pierote José Milton Matos Alessandra Ribeiro Walter Moura Ana Cristina Fialho |
| author_facet | Fabianne Soares Lima Luis Felipe Matos Isnayra Kerolaynne Pacheco Fernando Reis João Victor Frazão Câmara Josué Junior Araujo Pierote José Milton Matos Alessandra Ribeiro Walter Moura Ana Cristina Fialho |
| author_sort | Fabianne Soares Lima |
| collection | DOAJ |
| description | Abstract To analyze the biocompatibility of the scaffold produced from a natural polymer derived from castor oil through hemolytic activity and antimicrobial activity, to enable the clinical application. Three in vitro tests were performed: Hemolytic activity test - Polymer partially dissolved in contact with blood agar; Hemolytic activity test in sheep's blood - Polymer extract with red blood cells solution; Antimicrobial activity test - Solid polymer in direct contact with E. Coli and S. Aureus. For hemolytic tests, none of the samples showed hemolysis. Negative hemolytic activity is a good indicator, as the maintenance of the blood clot in the area of the lesion is essential for the formation of new tissue. For the antimicrobial activity test, no significant activity was observed against the bacteria used. The polymer is not toxic to red blood cells, being viable for clinical application as a matrix for tissue regeneration. |
| first_indexed | 2024-12-13T12:50:32Z |
| format | Article |
| id | doaj.art-7caf65d9ee2c4abaa6d5a7342ba8c9c7 |
| institution | Directory Open Access Journal |
| issn | 1678-5169 |
| language | English |
| last_indexed | 2024-12-13T12:50:32Z |
| publishDate | 2022-01-01 |
| publisher | Associação Brasileira de Polímeros |
| record_format | Article |
| series | Polímeros |
| spelling | doaj.art-7caf65d9ee2c4abaa6d5a7342ba8c9c72022-12-21T23:45:20ZengAssociação Brasileira de PolímerosPolímeros1678-51692022-01-0132110.1590/0104-1428.210018Scaffold based on castor oil as an osteoconductive matrix in bone repair: biocompatibility analysisFabianne Soares Limahttps://orcid.org/0000-0001-8070-7783Luis Felipe Matoshttps://orcid.org/0000-0002-7363-5176Isnayra Kerolaynne Pachecohttps://orcid.org/0000-0002-0494-0791Fernando Reishttps://orcid.org/0000-0002-7092-436XJoão Victor Frazão Câmarahttps://orcid.org/0000-0002-9687-4401Josué Junior Araujo Pierotehttps://orcid.org/0000-0003-0585-1405José Milton Matoshttps://orcid.org/0000-0003-3476-399XAlessandra Ribeirohttps://orcid.org/0000-0003-3822-8601Walter Mourahttps://orcid.org/0000-0001-6265-9415Ana Cristina Fialhohttps://orcid.org/0000-0002-2973-1080Abstract To analyze the biocompatibility of the scaffold produced from a natural polymer derived from castor oil through hemolytic activity and antimicrobial activity, to enable the clinical application. Three in vitro tests were performed: Hemolytic activity test - Polymer partially dissolved in contact with blood agar; Hemolytic activity test in sheep's blood - Polymer extract with red blood cells solution; Antimicrobial activity test - Solid polymer in direct contact with E. Coli and S. Aureus. For hemolytic tests, none of the samples showed hemolysis. Negative hemolytic activity is a good indicator, as the maintenance of the blood clot in the area of the lesion is essential for the formation of new tissue. For the antimicrobial activity test, no significant activity was observed against the bacteria used. The polymer is not toxic to red blood cells, being viable for clinical application as a matrix for tissue regeneration.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282022000100403&tlng=enbone matrixmaterials testingtissue scaffolds |
| spellingShingle | Fabianne Soares Lima Luis Felipe Matos Isnayra Kerolaynne Pacheco Fernando Reis João Victor Frazão Câmara Josué Junior Araujo Pierote José Milton Matos Alessandra Ribeiro Walter Moura Ana Cristina Fialho Scaffold based on castor oil as an osteoconductive matrix in bone repair: biocompatibility analysis Polímeros bone matrix materials testing tissue scaffolds |
| title | Scaffold based on castor oil as an osteoconductive matrix in bone repair: biocompatibility analysis |
| title_full | Scaffold based on castor oil as an osteoconductive matrix in bone repair: biocompatibility analysis |
| title_fullStr | Scaffold based on castor oil as an osteoconductive matrix in bone repair: biocompatibility analysis |
| title_full_unstemmed | Scaffold based on castor oil as an osteoconductive matrix in bone repair: biocompatibility analysis |
| title_short | Scaffold based on castor oil as an osteoconductive matrix in bone repair: biocompatibility analysis |
| title_sort | scaffold based on castor oil as an osteoconductive matrix in bone repair biocompatibility analysis |
| topic | bone matrix materials testing tissue scaffolds |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282022000100403&tlng=en |
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