Antibiofilm, Antimicrobial, Anti-Quorum Sensing, and Antioxidant Activities of Saudi Sidr Honey: In Vitro and Molecular Docking Studies
Sidr honey is a valuable source of bioactive compounds with promising biological properties. In the present study, antimicrobial, antioxidant, and anti-quorum sensing properties of Saudi Sidr honey were assessed, along with phytochemical analysis, via gas chromatography–mass spectrometry (GC-MS). In...
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MDPI AG
2023-08-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/9/2177 |
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| author | Abdulrahman S. Bazaid Ahmed Alsolami Mitesh Patel Aiah Mustafa Khateb Abdu Aldarhami Mejdi Snoussi Shekah M. Almusheet Husam Qanash |
| author_facet | Abdulrahman S. Bazaid Ahmed Alsolami Mitesh Patel Aiah Mustafa Khateb Abdu Aldarhami Mejdi Snoussi Shekah M. Almusheet Husam Qanash |
| author_sort | Abdulrahman S. Bazaid |
| collection | DOAJ |
| description | Sidr honey is a valuable source of bioactive compounds with promising biological properties. In the present study, antimicrobial, antioxidant, and anti-quorum sensing properties of Saudi Sidr honey were assessed, along with phytochemical analysis, via gas chromatography–mass spectrometry (GC-MS). In silico study was also carried out to study the drug-likeness properties of the identified compounds and to study their affinity with known target proteins assessed using molecular docking approach. The results showed that Saudi Sidr honey exhibited promising antibacterial activity, with MIC values ranging from 50 to 400 mg/mL and MBC values from 50 to >450 mg/mL. Interestingly, the Saudi Sidr honey was active against <i>Candida auris</i> and <i>Candida neoformans</i>, with an MIC value of about 500 mg/mL. Moreover, the Sidr honey showed important antioxidant activities (ABTS assay: IC50 5.41 ± 0.045 mg/mL; DPPH assay: IC50 7.70 ± 0.065 mg/mL) and <i>β</i>-carotene bleaching test results (IC50 ≥ 20 mg/mL). In addition, the Saudi Sidr honey was able to inhibit biofilm formation on glass slides at 1/2 MIC by 77.11% for <i>Bacillus subtilis</i>, 70.88% for <i>Staphylococcus aureus</i>, 61.79% for <i>Escherichia coli</i>, and 56.64% for <i>Pseudomonas aeruginosa</i>. Similarly, violacein production by <i>Chromobacterium violaceum</i> was reduced by about 56.63%, while the production of pyocyanin by <i>P. aeruginosa</i> was decreased to 46.27% at a low concentration of Saudi Sidr honey. ADMET properties showed that five identified compounds, namely, 1-cyclohexylimidazolidin-2-one, 3-Butyl-3-methylcyclohexanone, 4-butyl-3-methoxy-2-cyclo penten-1-one, 2,2,3,3-Tetramethyl cyclopropane carboxylic acid, and 3,5-dihydroxy-2-(3-methylbut-2-en-1-yl showed promising drug-likeness properties. The compound 3,5-dihydroxy-2-(3-methylbut-2-en-1-yl exhibited the highest binding energy against antimicrobial and antioxidant target proteins (1JIJ, 2VAM, 6B8A, 6F86, 2CDU, and 1OG5). Overall, the obtained results highlighted the promising potential of Saudi Sidr honey as a rich source of bioactive compounds that can be used as food preservatives and antimicrobial, antioxidant, and anti-quorum sensing molecules. |
| first_indexed | 2024-03-10T22:16:28Z |
| format | Article |
| id | doaj.art-7cb2f30b94144ce78df7a4254e0ad241 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T22:16:28Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-7cb2f30b94144ce78df7a4254e0ad2412023-11-19T12:26:03ZengMDPI AGPharmaceutics1999-49232023-08-01159217710.3390/pharmaceutics15092177Antibiofilm, Antimicrobial, Anti-Quorum Sensing, and Antioxidant Activities of Saudi Sidr Honey: In Vitro and Molecular Docking StudiesAbdulrahman S. Bazaid0Ahmed Alsolami1Mitesh Patel2Aiah Mustafa Khateb3Abdu Aldarhami4Mejdi Snoussi5Shekah M. Almusheet6Husam Qanash7Department of Medical Laboratory Science, College of Applied Medical Sciences, University of Ha’il, Hail 55476, Saudi ArabiaDepartment of Internal Medicine, College of Medicine, University of Ha’il, Hail 55476, Saudi ArabiaDepartment of Biotechnology, Parul Institute of Applied Sciences and Centre of Research for Development, Parul University, Vadodara 391760, Gujarat, IndiaMedical Laboratory Technology Department, College of Applied Medical Sciences, Taibah University, Madinah 42353, Saudi ArabiaDepartment of Medical Microbiology, Qunfudah Faculty of Medicine, Umm Al-Qura University, Al-Qunfudah 21961, Saudi ArabiaDepartment of Biology, College of Science, University of Ha’il, Hail 55476, Saudi ArabiaDepartment of Laboratory, King Khalid Hospital, Hail 55421, Saudi ArabiaDepartment of Medical Laboratory Science, College of Applied Medical Sciences, University of Ha’il, Hail 55476, Saudi ArabiaSidr honey is a valuable source of bioactive compounds with promising biological properties. In the present study, antimicrobial, antioxidant, and anti-quorum sensing properties of Saudi Sidr honey were assessed, along with phytochemical analysis, via gas chromatography–mass spectrometry (GC-MS). In silico study was also carried out to study the drug-likeness properties of the identified compounds and to study their affinity with known target proteins assessed using molecular docking approach. The results showed that Saudi Sidr honey exhibited promising antibacterial activity, with MIC values ranging from 50 to 400 mg/mL and MBC values from 50 to >450 mg/mL. Interestingly, the Saudi Sidr honey was active against <i>Candida auris</i> and <i>Candida neoformans</i>, with an MIC value of about 500 mg/mL. Moreover, the Sidr honey showed important antioxidant activities (ABTS assay: IC50 5.41 ± 0.045 mg/mL; DPPH assay: IC50 7.70 ± 0.065 mg/mL) and <i>β</i>-carotene bleaching test results (IC50 ≥ 20 mg/mL). In addition, the Saudi Sidr honey was able to inhibit biofilm formation on glass slides at 1/2 MIC by 77.11% for <i>Bacillus subtilis</i>, 70.88% for <i>Staphylococcus aureus</i>, 61.79% for <i>Escherichia coli</i>, and 56.64% for <i>Pseudomonas aeruginosa</i>. Similarly, violacein production by <i>Chromobacterium violaceum</i> was reduced by about 56.63%, while the production of pyocyanin by <i>P. aeruginosa</i> was decreased to 46.27% at a low concentration of Saudi Sidr honey. ADMET properties showed that five identified compounds, namely, 1-cyclohexylimidazolidin-2-one, 3-Butyl-3-methylcyclohexanone, 4-butyl-3-methoxy-2-cyclo penten-1-one, 2,2,3,3-Tetramethyl cyclopropane carboxylic acid, and 3,5-dihydroxy-2-(3-methylbut-2-en-1-yl showed promising drug-likeness properties. The compound 3,5-dihydroxy-2-(3-methylbut-2-en-1-yl exhibited the highest binding energy against antimicrobial and antioxidant target proteins (1JIJ, 2VAM, 6B8A, 6F86, 2CDU, and 1OG5). Overall, the obtained results highlighted the promising potential of Saudi Sidr honey as a rich source of bioactive compounds that can be used as food preservatives and antimicrobial, antioxidant, and anti-quorum sensing molecules.https://www.mdpi.com/1999-4923/15/9/2177Saudi Sidr honeyantimicrobialantioxidantanti-quorumADMET |
| spellingShingle | Abdulrahman S. Bazaid Ahmed Alsolami Mitesh Patel Aiah Mustafa Khateb Abdu Aldarhami Mejdi Snoussi Shekah M. Almusheet Husam Qanash Antibiofilm, Antimicrobial, Anti-Quorum Sensing, and Antioxidant Activities of Saudi Sidr Honey: In Vitro and Molecular Docking Studies Pharmaceutics Saudi Sidr honey antimicrobial antioxidant anti-quorum ADMET |
| title | Antibiofilm, Antimicrobial, Anti-Quorum Sensing, and Antioxidant Activities of Saudi Sidr Honey: In Vitro and Molecular Docking Studies |
| title_full | Antibiofilm, Antimicrobial, Anti-Quorum Sensing, and Antioxidant Activities of Saudi Sidr Honey: In Vitro and Molecular Docking Studies |
| title_fullStr | Antibiofilm, Antimicrobial, Anti-Quorum Sensing, and Antioxidant Activities of Saudi Sidr Honey: In Vitro and Molecular Docking Studies |
| title_full_unstemmed | Antibiofilm, Antimicrobial, Anti-Quorum Sensing, and Antioxidant Activities of Saudi Sidr Honey: In Vitro and Molecular Docking Studies |
| title_short | Antibiofilm, Antimicrobial, Anti-Quorum Sensing, and Antioxidant Activities of Saudi Sidr Honey: In Vitro and Molecular Docking Studies |
| title_sort | antibiofilm antimicrobial anti quorum sensing and antioxidant activities of saudi sidr honey in vitro and molecular docking studies |
| topic | Saudi Sidr honey antimicrobial antioxidant anti-quorum ADMET |
| url | https://www.mdpi.com/1999-4923/15/9/2177 |
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