Temporal Hierarchy and Context-Dependence of Quorum Sensing Signal in <i>Pseudomonas aeruginosa</i>
The Gram-negative bacterium <i>Pseudomonas aeruginosa</i> can cause infections in a broad range of hosts including plants, invertebrates and mammals and is an important source of nosocomial infections in humans. We were interested in how differences in the bacteria’s nutritional environm...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/12/12/1953 |
| _version_ | 1827638115429253120 |
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| author | Stoyko Katzarov Volker Behrends |
| author_facet | Stoyko Katzarov Volker Behrends |
| author_sort | Stoyko Katzarov |
| collection | DOAJ |
| description | The Gram-negative bacterium <i>Pseudomonas aeruginosa</i> can cause infections in a broad range of hosts including plants, invertebrates and mammals and is an important source of nosocomial infections in humans. We were interested in how differences in the bacteria’s nutritional environment impact bacterial communication and virulence factor production. We grew <i>P. aeruginosa</i> in 96 different conditions in BIOLOG Gen III plates and assayed quorum sensing (QS) signaling over the course of growth. We also quantified pyocyanin and biofilm production and the impact of sub-inhibitory exposure to tobramycin. We found that while 3-oxo-C12 homoserine lactone remained the dominant QS signal to be produced, timing of PQS production differed between media types. Further, whether cells grew predominantly as biofilms or planktonic cells was highly context dependent. Our data suggest that understanding the impact of the nutritional environment on the bacterium can lead to valuable insights into the link between bacterial physiology and pathology. |
| first_indexed | 2024-03-09T16:12:04Z |
| format | Article |
| id | doaj.art-7cb5ddb06d1a4244bb56e5c477ddd553 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T16:12:04Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-7cb5ddb06d1a4244bb56e5c477ddd5532023-11-24T16:11:01ZengMDPI AGLife2075-17292022-11-011212195310.3390/life12121953Temporal Hierarchy and Context-Dependence of Quorum Sensing Signal in <i>Pseudomonas aeruginosa</i>Stoyko Katzarov0Volker Behrends1The Centre for Integrated Research in Life and Health Sciences, University of Roehampton, London SW15 4JD, UKThe Centre for Integrated Research in Life and Health Sciences, University of Roehampton, London SW15 4JD, UKThe Gram-negative bacterium <i>Pseudomonas aeruginosa</i> can cause infections in a broad range of hosts including plants, invertebrates and mammals and is an important source of nosocomial infections in humans. We were interested in how differences in the bacteria’s nutritional environment impact bacterial communication and virulence factor production. We grew <i>P. aeruginosa</i> in 96 different conditions in BIOLOG Gen III plates and assayed quorum sensing (QS) signaling over the course of growth. We also quantified pyocyanin and biofilm production and the impact of sub-inhibitory exposure to tobramycin. We found that while 3-oxo-C12 homoserine lactone remained the dominant QS signal to be produced, timing of PQS production differed between media types. Further, whether cells grew predominantly as biofilms or planktonic cells was highly context dependent. Our data suggest that understanding the impact of the nutritional environment on the bacterium can lead to valuable insights into the link between bacterial physiology and pathology.https://www.mdpi.com/2075-1729/12/12/1953mass spectrometryESKAPE pathogens<i>Pseudomonas aeruginosa</i>quorum sensinghierarchysub-MIC antibiotics |
| spellingShingle | Stoyko Katzarov Volker Behrends Temporal Hierarchy and Context-Dependence of Quorum Sensing Signal in <i>Pseudomonas aeruginosa</i> Life mass spectrometry ESKAPE pathogens <i>Pseudomonas aeruginosa</i> quorum sensing hierarchy sub-MIC antibiotics |
| title | Temporal Hierarchy and Context-Dependence of Quorum Sensing Signal in <i>Pseudomonas aeruginosa</i> |
| title_full | Temporal Hierarchy and Context-Dependence of Quorum Sensing Signal in <i>Pseudomonas aeruginosa</i> |
| title_fullStr | Temporal Hierarchy and Context-Dependence of Quorum Sensing Signal in <i>Pseudomonas aeruginosa</i> |
| title_full_unstemmed | Temporal Hierarchy and Context-Dependence of Quorum Sensing Signal in <i>Pseudomonas aeruginosa</i> |
| title_short | Temporal Hierarchy and Context-Dependence of Quorum Sensing Signal in <i>Pseudomonas aeruginosa</i> |
| title_sort | temporal hierarchy and context dependence of quorum sensing signal in i pseudomonas aeruginosa i |
| topic | mass spectrometry ESKAPE pathogens <i>Pseudomonas aeruginosa</i> quorum sensing hierarchy sub-MIC antibiotics |
| url | https://www.mdpi.com/2075-1729/12/12/1953 |
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