Synovial Extracellular Vesicles: Structure and Role in Synovial Fluid Tribological Performances
The quality of the lubricant between cartilaginous joint surfaces impacts the joint’s mechanistic properties. In this study, we define the biochemical, ultrastructural, and tribological signatures of synovial fluids (SF) from patients with degenerative (osteoarthritis-OA) or inflammatory (rheumatoid...
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/19/11998 |
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| author | Layth Ben-Trad Constantin Ionut Matei Mirela Maria Sava Samira Filali Marie-Eve Duclos Yves Berthier Michel Guichardant Nathalie Bernoud-Hubac Ofelia Maniti Ahmed Landoulsi Marie-Genevieve Blanchin Pierre Miossec Thierry Granjon Ana-Maria Trunfio-Sfarghiu |
| author_facet | Layth Ben-Trad Constantin Ionut Matei Mirela Maria Sava Samira Filali Marie-Eve Duclos Yves Berthier Michel Guichardant Nathalie Bernoud-Hubac Ofelia Maniti Ahmed Landoulsi Marie-Genevieve Blanchin Pierre Miossec Thierry Granjon Ana-Maria Trunfio-Sfarghiu |
| author_sort | Layth Ben-Trad |
| collection | DOAJ |
| description | The quality of the lubricant between cartilaginous joint surfaces impacts the joint’s mechanistic properties. In this study, we define the biochemical, ultrastructural, and tribological signatures of synovial fluids (SF) from patients with degenerative (osteoarthritis-OA) or inflammatory (rheumatoid arthritis-RA) joint pathologies in comparison with SF from healthy subjects. Phospholipid (PL) concentration in SF increased in pathological contexts, but the proportion PL relative to the overall lipids decreased. Subtle changes in PL chain composition were attributed to the inflammatory state. Transmission electron microscopy showed the occurrence of large multilamellar synovial extracellular vesicles (EV) filled with glycoprotein gel in healthy subjects. Synovial extracellular vesicle structure was altered in SF from OA and RA patients. RA samples systematically showed lower viscosity than healthy samples under a hydrodynamic lubricating regimen whereas OA samples showed higher viscosity. In turn, under a boundary regimen, cartilage surfaces in both pathological situations showed high wear and friction coefficients. Thus, we found a difference in the biochemical, tribological, and ultrastructural properties of synovial fluid in healthy people and patients with osteoarthritis and arthritis of the joints, and that large, multilamellar vesicles are essential for good boundary lubrication by ensuring a ball-bearing effect and limiting the destruction of lipid layers at the cartilage surface. |
| first_indexed | 2024-03-09T21:36:22Z |
| format | Article |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:36:22Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7cb6c43c9855452a977d7d710e639fd22023-11-23T20:42:49ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123191199810.3390/ijms231911998Synovial Extracellular Vesicles: Structure and Role in Synovial Fluid Tribological PerformancesLayth Ben-Trad0Constantin Ionut Matei1Mirela Maria Sava2Samira Filali3Marie-Eve Duclos4Yves Berthier5Michel Guichardant6Nathalie Bernoud-Hubac7Ofelia Maniti8Ahmed Landoulsi9Marie-Genevieve Blanchin10Pierre Miossec11Thierry Granjon12Ana-Maria Trunfio-Sfarghiu13Laboratory of Contact and Structural Mechanics, University of Lyon, CNRS, INSA Lyon, UMR5259, Villeurbanne, 69100 Lyon, FranceLaboratory of Contact and Structural Mechanics, University of Lyon, CNRS, INSA Lyon, UMR5259, Villeurbanne, 69100 Lyon, FranceLaboratory of Contact and Structural Mechanics, University of Lyon, CNRS, INSA Lyon, UMR5259, Villeurbanne, 69100 Lyon, FranceUnit of Immunogenetics & Inflammation EA-4130 & Department of Clinical Immunology and Rheumatology, University of Lyon, Hôpital Edouard Herriot, 69437 Lyon, FranceCharles River Laboratories, 13, Allée de Nudlingen, 27950 Saint-Marcel, FranceLaboratory of Contact and Structural Mechanics, University of Lyon, CNRS, INSA Lyon, UMR5259, Villeurbanne, 69100 Lyon, FranceLaboratory of Contact and Structural Mechanics, University of Lyon, CNRS, INSA Lyon, UMR5259, Villeurbanne, 69100 Lyon, FranceLaboratory of Contact and Structural Mechanics, University of Lyon, CNRS, INSA Lyon, UMR5259, Villeurbanne, 69100 Lyon, FranceInstitute de Chimie et Biochimie Moléculaires et Supramoléculaires, ICBMS UMR 5246, University of Lyon, Université Lyon 1, CNRS, 69622 Lyon, FranceFaculty of Sciences of Bizerte, University of Carthage, Laboratory of Risques Liés aux Stress Environnementaux: Lutte et Prévention, Zarzouna 1054, TunisiaInstitute Lumiere Mat, University of Lyon, CNRS, UCBL, ILM, UMR5506, 69622 Villeurbanne, FranceUnit of Immunogenetics & Inflammation EA-4130 & Department of Clinical Immunology and Rheumatology, University of Lyon, Hôpital Edouard Herriot, 69437 Lyon, FranceInstitute de Chimie et Biochimie Moléculaires et Supramoléculaires, ICBMS UMR 5246, University of Lyon, Université Lyon 1, CNRS, 69622 Lyon, FranceLaboratory of Contact and Structural Mechanics, University of Lyon, CNRS, INSA Lyon, UMR5259, Villeurbanne, 69100 Lyon, FranceThe quality of the lubricant between cartilaginous joint surfaces impacts the joint’s mechanistic properties. In this study, we define the biochemical, ultrastructural, and tribological signatures of synovial fluids (SF) from patients with degenerative (osteoarthritis-OA) or inflammatory (rheumatoid arthritis-RA) joint pathologies in comparison with SF from healthy subjects. Phospholipid (PL) concentration in SF increased in pathological contexts, but the proportion PL relative to the overall lipids decreased. Subtle changes in PL chain composition were attributed to the inflammatory state. Transmission electron microscopy showed the occurrence of large multilamellar synovial extracellular vesicles (EV) filled with glycoprotein gel in healthy subjects. Synovial extracellular vesicle structure was altered in SF from OA and RA patients. RA samples systematically showed lower viscosity than healthy samples under a hydrodynamic lubricating regimen whereas OA samples showed higher viscosity. In turn, under a boundary regimen, cartilage surfaces in both pathological situations showed high wear and friction coefficients. Thus, we found a difference in the biochemical, tribological, and ultrastructural properties of synovial fluid in healthy people and patients with osteoarthritis and arthritis of the joints, and that large, multilamellar vesicles are essential for good boundary lubrication by ensuring a ball-bearing effect and limiting the destruction of lipid layers at the cartilage surface.https://www.mdpi.com/1422-0067/23/19/11998synovial fluidlubricantrheumatoid arthritisosteoarthritisnanoparticleslipid structures |
| spellingShingle | Layth Ben-Trad Constantin Ionut Matei Mirela Maria Sava Samira Filali Marie-Eve Duclos Yves Berthier Michel Guichardant Nathalie Bernoud-Hubac Ofelia Maniti Ahmed Landoulsi Marie-Genevieve Blanchin Pierre Miossec Thierry Granjon Ana-Maria Trunfio-Sfarghiu Synovial Extracellular Vesicles: Structure and Role in Synovial Fluid Tribological Performances International Journal of Molecular Sciences synovial fluid lubricant rheumatoid arthritis osteoarthritis nanoparticles lipid structures |
| title | Synovial Extracellular Vesicles: Structure and Role in Synovial Fluid Tribological Performances |
| title_full | Synovial Extracellular Vesicles: Structure and Role in Synovial Fluid Tribological Performances |
| title_fullStr | Synovial Extracellular Vesicles: Structure and Role in Synovial Fluid Tribological Performances |
| title_full_unstemmed | Synovial Extracellular Vesicles: Structure and Role in Synovial Fluid Tribological Performances |
| title_short | Synovial Extracellular Vesicles: Structure and Role in Synovial Fluid Tribological Performances |
| title_sort | synovial extracellular vesicles structure and role in synovial fluid tribological performances |
| topic | synovial fluid lubricant rheumatoid arthritis osteoarthritis nanoparticles lipid structures |
| url | https://www.mdpi.com/1422-0067/23/19/11998 |
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