Effects of Exogenous Proteins Injection into the Bursa of Fabricius on Humoral Immunity in Neonatal Chickens
Early researches have shown that the bursa of Fabricius is a critical organ for development of B lymphocytes in birds. Exogenous proteins from the gut lumen or the environment are taken up by the follicle-associated epithelium and enter into the bursal follicle. Not all of the B lymphocytes in the...
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2012-04-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/49/2/49_011078/_pdf/-char/en |
| _version_ | 1827938027667718144 |
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| author | Fang Yuan Guojin Wu Junshuang Gao Xiaoyan Tang Zandong Li |
| author_facet | Fang Yuan Guojin Wu Junshuang Gao Xiaoyan Tang Zandong Li |
| author_sort | Fang Yuan |
| collection | DOAJ |
| description | Early researches have shown that the bursa of Fabricius is a critical organ for development of B lymphocytes in birds. Exogenous proteins from the gut lumen or the environment are taken up by the follicle-associated epithelium and enter into the bursal follicle. Not all of the B lymphocytes in the bursal follicle mature and emigrate to the periphery and antigen-dependent selection for B lymphocytes may occur in the bursa. However, the actual impact of antigens in the bursa on B cells is not clear. In this study, dinitrophenyl (DNP) or 2,4,6-trinitrophenyl (TNP) coupled bovine serum albumin (BSA) was injected into the bursa of one-day-old chickens, a time when most B lymphocytes in the bursal follicles begun to emigrate from the medulla to the cortex. Immunohistochemical analysis showed that DNP-BSA was only distributed in the bursal medulla. By injecting TNP-BSA into the bursa of one-day-old chickens, and immunizing the chickens with TNP-HSA 3 weeks later, we found that injection of TNP-BSA increased anti-TNP titers in the sera of chickens after immunization. Taken together, the results suggest that the bursa is the site of B cell-antigen interaction and is capable of causing Ag-specific B cell emigration and increasing an antigen-specific immune response at the B cell level. |
| first_indexed | 2024-03-13T08:34:01Z |
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| id | doaj.art-7cb75276068c4ff1a80cf91251550971 |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:34:01Z |
| publishDate | 2012-04-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-7cb75276068c4ff1a80cf912515509712023-05-31T01:13:16ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862012-04-0149212412910.2141/jpsa.011078jpsaEffects of Exogenous Proteins Injection into the Bursa of Fabricius on Humoral Immunity in Neonatal ChickensFang Yuan0Guojin Wu1Junshuang Gao2Xiaoyan Tang3Zandong Li4State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Science, China Agricultural University, ChinaState Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Science, China Agricultural University, ChinaState Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Science, China Agricultural University, ChinaState Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Science, China Agricultural University, ChinaState Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Science, China Agricultural University, ChinaEarly researches have shown that the bursa of Fabricius is a critical organ for development of B lymphocytes in birds. Exogenous proteins from the gut lumen or the environment are taken up by the follicle-associated epithelium and enter into the bursal follicle. Not all of the B lymphocytes in the bursal follicle mature and emigrate to the periphery and antigen-dependent selection for B lymphocytes may occur in the bursa. However, the actual impact of antigens in the bursa on B cells is not clear. In this study, dinitrophenyl (DNP) or 2,4,6-trinitrophenyl (TNP) coupled bovine serum albumin (BSA) was injected into the bursa of one-day-old chickens, a time when most B lymphocytes in the bursal follicles begun to emigrate from the medulla to the cortex. Immunohistochemical analysis showed that DNP-BSA was only distributed in the bursal medulla. By injecting TNP-BSA into the bursa of one-day-old chickens, and immunizing the chickens with TNP-HSA 3 weeks later, we found that injection of TNP-BSA increased anti-TNP titers in the sera of chickens after immunization. Taken together, the results suggest that the bursa is the site of B cell-antigen interaction and is capable of causing Ag-specific B cell emigration and increasing an antigen-specific immune response at the B cell level.https://www.jstage.jst.go.jp/article/jpsa/49/2/49_011078/_pdf/-char/enb cellbursa of fabriciuschickenexogenous proteinhumoral immunity |
| spellingShingle | Fang Yuan Guojin Wu Junshuang Gao Xiaoyan Tang Zandong Li Effects of Exogenous Proteins Injection into the Bursa of Fabricius on Humoral Immunity in Neonatal Chickens The Journal of Poultry Science b cell bursa of fabricius chicken exogenous protein humoral immunity |
| title | Effects of Exogenous Proteins Injection into the Bursa of Fabricius on Humoral Immunity in Neonatal Chickens |
| title_full | Effects of Exogenous Proteins Injection into the Bursa of Fabricius on Humoral Immunity in Neonatal Chickens |
| title_fullStr | Effects of Exogenous Proteins Injection into the Bursa of Fabricius on Humoral Immunity in Neonatal Chickens |
| title_full_unstemmed | Effects of Exogenous Proteins Injection into the Bursa of Fabricius on Humoral Immunity in Neonatal Chickens |
| title_short | Effects of Exogenous Proteins Injection into the Bursa of Fabricius on Humoral Immunity in Neonatal Chickens |
| title_sort | effects of exogenous proteins injection into the bursa of fabricius on humoral immunity in neonatal chickens |
| topic | b cell bursa of fabricius chicken exogenous protein humoral immunity |
| url | https://www.jstage.jst.go.jp/article/jpsa/49/2/49_011078/_pdf/-char/en |
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