Protocol for Patch-Seq of Small Interneurons
Summary: Obtaining electrophysiological recordings and gene expression information from the same neuron (Patch-seq) brings forward a unique opportunity to study the transcriptional correlates of functional properties and vice versa. Here, we provide a detailed Patch-seq protocol tailored to the spec...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-12-01
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| Series: | STAR Protocols |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720301337 |
| _version_ | 1818600870959382528 |
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| author | Marcela Lipovsek Lorcan Browne Matthew S. Grubb |
| author_facet | Marcela Lipovsek Lorcan Browne Matthew S. Grubb |
| author_sort | Marcela Lipovsek |
| collection | DOAJ |
| description | Summary: Obtaining electrophysiological recordings and gene expression information from the same neuron (Patch-seq) brings forward a unique opportunity to study the transcriptional correlates of functional properties and vice versa. Here, we provide a detailed Patch-seq protocol tailored to the specialized demands of studying small interneurons. Focusing on the technically demanding process of transitioning between patch recordings and cell extraction, our protocol describes and troubleshoots steps for successfully collecting small interneurons, allowing for multi-modal Patch-seq interrogation of this crucial cell type. |
| first_indexed | 2024-12-16T12:42:22Z |
| format | Article |
| id | doaj.art-7cbaee99283b4f07be46e69affe7b93e |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-16T12:42:22Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-7cbaee99283b4f07be46e69affe7b93e2022-12-21T22:31:22ZengElsevierSTAR Protocols2666-16672020-12-0113100146Protocol for Patch-Seq of Small InterneuronsMarcela Lipovsek0Lorcan Browne1Matthew S. Grubb2Centre for Developmental Neurobiology, Institute of Psychiatry, Psychology and Neuroscience (IoPPN), King’s College London, London, UK; Corresponding authorCentre for Developmental Neurobiology, Institute of Psychiatry, Psychology and Neuroscience (IoPPN), King’s College London, London, UKCentre for Developmental Neurobiology, Institute of Psychiatry, Psychology and Neuroscience (IoPPN), King’s College London, London, UK; Corresponding authorSummary: Obtaining electrophysiological recordings and gene expression information from the same neuron (Patch-seq) brings forward a unique opportunity to study the transcriptional correlates of functional properties and vice versa. Here, we provide a detailed Patch-seq protocol tailored to the specialized demands of studying small interneurons. Focusing on the technically demanding process of transitioning between patch recordings and cell extraction, our protocol describes and troubleshoots steps for successfully collecting small interneurons, allowing for multi-modal Patch-seq interrogation of this crucial cell type.http://www.sciencedirect.com/science/article/pii/S2666166720301337 |
| spellingShingle | Marcela Lipovsek Lorcan Browne Matthew S. Grubb Protocol for Patch-Seq of Small Interneurons STAR Protocols |
| title | Protocol for Patch-Seq of Small Interneurons |
| title_full | Protocol for Patch-Seq of Small Interneurons |
| title_fullStr | Protocol for Patch-Seq of Small Interneurons |
| title_full_unstemmed | Protocol for Patch-Seq of Small Interneurons |
| title_short | Protocol for Patch-Seq of Small Interneurons |
| title_sort | protocol for patch seq of small interneurons |
| url | http://www.sciencedirect.com/science/article/pii/S2666166720301337 |
| work_keys_str_mv | AT marcelalipovsek protocolforpatchseqofsmallinterneurons AT lorcanbrowne protocolforpatchseqofsmallinterneurons AT matthewsgrubb protocolforpatchseqofsmallinterneurons |