Extraction methods of fat from food samples and preparation of fatty acid methyl esters for gas chromatography: A review

Global trends moved towards fast food consumption due to the busy lifestyle of humans. Hence, the intake of fat-related food has exceeded the daily dietary reference intake (DRI) of fat, which caused multiple diseases. Analysis of the fatty acid profile plays a vital role in nutritional labelling an...

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Main Authors: Geeth G. Hewavitharana, Dilini N. Perera, S.B. Navaratne, I. Wickramasinghe
Format: Article
Language:English
Published: Elsevier 2020-08-01
Series:Arabian Journal of Chemistry
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1878535220302458
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author Geeth G. Hewavitharana
Dilini N. Perera
S.B. Navaratne
I. Wickramasinghe
author_facet Geeth G. Hewavitharana
Dilini N. Perera
S.B. Navaratne
I. Wickramasinghe
author_sort Geeth G. Hewavitharana
collection DOAJ
description Global trends moved towards fast food consumption due to the busy lifestyle of humans. Hence, the intake of fat-related food has exceeded the daily dietary reference intake (DRI) of fat, which caused multiple diseases. Analysis of the fatty acid profile plays a vital role in nutritional labelling and helps to understand the availability of diverse fatty acids among food commodities. This article reviews, general fatty acid extraction and derivatization techniques that have been developed in the past few decades due to the structural differences of fatty acids and briefed the steps involved in the complete process of fatty acid analysis using gas chromatography-mass spectrometry (GC–MS). Hence, the review mainly focused on conventional extraction methods, followed by microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and supercritical fluid extraction (SFE) methods and widely used acid, base, and modified derivatization techniques. Importantly, this article compares the results of the previous studies in each section which assist to decide on the most appropriate pathway for the fatty acid analysis for different selected food types. Therefore, it is hoped that this review may help researchers to develop existing experimental methods and to improve ‘bad’ fatty acid level mitigation techniques in future.
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spelling doaj.art-7cbbdd2641be462aa8deb1bfdc7173f42022-12-22T01:13:24ZengElsevierArabian Journal of Chemistry1878-53522020-08-0113868656875Extraction methods of fat from food samples and preparation of fatty acid methyl esters for gas chromatography: A reviewGeeth G. Hewavitharana0Dilini N. Perera1S.B. Navaratne2I. Wickramasinghe3Corresponding author.; Department of Food Science and Technology, University of Sri Jayewardenepura, Nugegoda 10250, Sri LankaDepartment of Food Science and Technology, University of Sri Jayewardenepura, Nugegoda 10250, Sri LankaDepartment of Food Science and Technology, University of Sri Jayewardenepura, Nugegoda 10250, Sri LankaDepartment of Food Science and Technology, University of Sri Jayewardenepura, Nugegoda 10250, Sri LankaGlobal trends moved towards fast food consumption due to the busy lifestyle of humans. Hence, the intake of fat-related food has exceeded the daily dietary reference intake (DRI) of fat, which caused multiple diseases. Analysis of the fatty acid profile plays a vital role in nutritional labelling and helps to understand the availability of diverse fatty acids among food commodities. This article reviews, general fatty acid extraction and derivatization techniques that have been developed in the past few decades due to the structural differences of fatty acids and briefed the steps involved in the complete process of fatty acid analysis using gas chromatography-mass spectrometry (GC–MS). Hence, the review mainly focused on conventional extraction methods, followed by microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and supercritical fluid extraction (SFE) methods and widely used acid, base, and modified derivatization techniques. Importantly, this article compares the results of the previous studies in each section which assist to decide on the most appropriate pathway for the fatty acid analysis for different selected food types. Therefore, it is hoped that this review may help researchers to develop existing experimental methods and to improve ‘bad’ fatty acid level mitigation techniques in future.http://www.sciencedirect.com/science/article/pii/S1878535220302458Gas chromatographyFatty acidExtraction methodsDerivatizationFAME
spellingShingle Geeth G. Hewavitharana
Dilini N. Perera
S.B. Navaratne
I. Wickramasinghe
Extraction methods of fat from food samples and preparation of fatty acid methyl esters for gas chromatography: A review
Arabian Journal of Chemistry
Gas chromatography
Fatty acid
Extraction methods
Derivatization
FAME
title Extraction methods of fat from food samples and preparation of fatty acid methyl esters for gas chromatography: A review
title_full Extraction methods of fat from food samples and preparation of fatty acid methyl esters for gas chromatography: A review
title_fullStr Extraction methods of fat from food samples and preparation of fatty acid methyl esters for gas chromatography: A review
title_full_unstemmed Extraction methods of fat from food samples and preparation of fatty acid methyl esters for gas chromatography: A review
title_short Extraction methods of fat from food samples and preparation of fatty acid methyl esters for gas chromatography: A review
title_sort extraction methods of fat from food samples and preparation of fatty acid methyl esters for gas chromatography a review
topic Gas chromatography
Fatty acid
Extraction methods
Derivatization
FAME
url http://www.sciencedirect.com/science/article/pii/S1878535220302458
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AT sbnavaratne extractionmethodsoffatfromfoodsamplesandpreparationoffattyacidmethylestersforgaschromatographyareview
AT iwickramasinghe extractionmethodsoffatfromfoodsamplesandpreparationoffattyacidmethylestersforgaschromatographyareview