Comparison of sublingual microcirculatory parameters measured by sidestream darkfield videomicroscopy in anesthetized pigs and adult humans
Abstract Background This study aimed to compare sublingual microcirculatory parameters between anesthetized pigs and conscious adult humans using sidestream darkfield videomicroscopy. The overarching aim of the work was to validate the pig as an experimental model of changes in microcirculatory func...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-10-01
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| Series: | Animal Models and Experimental Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ame2.12348 |
| _version_ | 1797645437250830336 |
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| author | Raushan Lala Ryan Homes Shaun Pratt Wendy Goodwin Mark Midwinter |
| author_facet | Raushan Lala Ryan Homes Shaun Pratt Wendy Goodwin Mark Midwinter |
| author_sort | Raushan Lala |
| collection | DOAJ |
| description | Abstract Background This study aimed to compare sublingual microcirculatory parameters between anesthetized pigs and conscious adult humans using sidestream darkfield videomicroscopy. The overarching aim of the work was to validate the pig as an experimental model of changes in microcirculatory function following traumatic haemorrhagic shock and resuscitation. Methods Fourteen large white pigs and 14 humans were recruited for the study. Sublingual sidestream darkfield videomicroscopy clips were captured in anesthetized pigs and conscious humans. Clips underwent manual analysis in Automated Vascular Analysis 3.2 software. The total vessel density (TVD), perfused vessel density (PVD), proportion of perfused vessels (PPVs) and microvascular flow index (MFI) were quantified. An independent samples t test was used for between species comparison of microcirculatory parameters. Results and Conclusions Conscious humans had a significantly lower TVD, PVD and MFI than anesthetized pigs. No significant difference in PPVs was observed between the species. Perfusion of the microcirculation is a critical determinant of tissue metabolic function and viability. Whilst it may not be surprising that some interspecies differences in the sublingual microcirculatory anatomy were identified between pig and human subjects, it is interesting to report the insignificant difference in PPVs. This direct microcirculatory measure represents a relative change which should hold translatable value across species. We therefore conclude the pig is a suitable model for microcirculatory research and may be a suitable species to investigate changes in microcirculatory perfusion following perturbations in cardiovascular homeostasis, for example during traumatic haemorrhagic shock and resuscitation. |
| first_indexed | 2024-03-11T14:47:15Z |
| format | Article |
| id | doaj.art-7cbc866813b746c5ada1f73988f2d00a |
| institution | Directory Open Access Journal |
| issn | 2576-2095 |
| language | English |
| last_indexed | 2024-03-11T14:47:15Z |
| publishDate | 2023-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Animal Models and Experimental Medicine |
| spelling | doaj.art-7cbc866813b746c5ada1f73988f2d00a2023-10-30T10:48:26ZengWileyAnimal Models and Experimental Medicine2576-20952023-10-016549950310.1002/ame2.12348Comparison of sublingual microcirculatory parameters measured by sidestream darkfield videomicroscopy in anesthetized pigs and adult humansRaushan Lala0Ryan Homes1Shaun Pratt2Wendy Goodwin3Mark Midwinter4School of Biomedical Sciences The University of Queensland St Lucia Qld AustraliaSchool of Biomedical Sciences The University of Queensland St Lucia Qld AustraliaSchool of Veterinary Sciences The University of Queensland Gatton Qld AustraliaSchool of Veterinary Sciences The University of Queensland Gatton Qld AustraliaSchool of Biomedical Sciences The University of Queensland St Lucia Qld AustraliaAbstract Background This study aimed to compare sublingual microcirculatory parameters between anesthetized pigs and conscious adult humans using sidestream darkfield videomicroscopy. The overarching aim of the work was to validate the pig as an experimental model of changes in microcirculatory function following traumatic haemorrhagic shock and resuscitation. Methods Fourteen large white pigs and 14 humans were recruited for the study. Sublingual sidestream darkfield videomicroscopy clips were captured in anesthetized pigs and conscious humans. Clips underwent manual analysis in Automated Vascular Analysis 3.2 software. The total vessel density (TVD), perfused vessel density (PVD), proportion of perfused vessels (PPVs) and microvascular flow index (MFI) were quantified. An independent samples t test was used for between species comparison of microcirculatory parameters. Results and Conclusions Conscious humans had a significantly lower TVD, PVD and MFI than anesthetized pigs. No significant difference in PPVs was observed between the species. Perfusion of the microcirculation is a critical determinant of tissue metabolic function and viability. Whilst it may not be surprising that some interspecies differences in the sublingual microcirculatory anatomy were identified between pig and human subjects, it is interesting to report the insignificant difference in PPVs. This direct microcirculatory measure represents a relative change which should hold translatable value across species. We therefore conclude the pig is a suitable model for microcirculatory research and may be a suitable species to investigate changes in microcirculatory perfusion following perturbations in cardiovascular homeostasis, for example during traumatic haemorrhagic shock and resuscitation.https://doi.org/10.1002/ame2.12348comparative anatomymicrocirculationsidestream darkfield videomicroscopy |
| spellingShingle | Raushan Lala Ryan Homes Shaun Pratt Wendy Goodwin Mark Midwinter Comparison of sublingual microcirculatory parameters measured by sidestream darkfield videomicroscopy in anesthetized pigs and adult humans Animal Models and Experimental Medicine comparative anatomy microcirculation sidestream darkfield videomicroscopy |
| title | Comparison of sublingual microcirculatory parameters measured by sidestream darkfield videomicroscopy in anesthetized pigs and adult humans |
| title_full | Comparison of sublingual microcirculatory parameters measured by sidestream darkfield videomicroscopy in anesthetized pigs and adult humans |
| title_fullStr | Comparison of sublingual microcirculatory parameters measured by sidestream darkfield videomicroscopy in anesthetized pigs and adult humans |
| title_full_unstemmed | Comparison of sublingual microcirculatory parameters measured by sidestream darkfield videomicroscopy in anesthetized pigs and adult humans |
| title_short | Comparison of sublingual microcirculatory parameters measured by sidestream darkfield videomicroscopy in anesthetized pigs and adult humans |
| title_sort | comparison of sublingual microcirculatory parameters measured by sidestream darkfield videomicroscopy in anesthetized pigs and adult humans |
| topic | comparative anatomy microcirculation sidestream darkfield videomicroscopy |
| url | https://doi.org/10.1002/ame2.12348 |
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