Multi-generation reproductive toxicity of RDX and the involved signal pathways in Caenorhabditis elegans
As one of the most frequently used explosives, hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) can cause persistent pollution in the environment, leading to the potential ecological threat crossing the generations. In this study, we employed Caenorhabditis elegans to explore the toxic effects of RDX o...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S014765132300578X |
| _version_ | 1827928343171825664 |
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| author | Lingyan Sun Yanping Zhou Chunyan Wang Yaguang Nie An Xu Lijun Wu |
| author_facet | Lingyan Sun Yanping Zhou Chunyan Wang Yaguang Nie An Xu Lijun Wu |
| author_sort | Lingyan Sun |
| collection | DOAJ |
| description | As one of the most frequently used explosives, hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) can cause persistent pollution in the environment, leading to the potential ecological threat crossing the generations. In this study, we employed Caenorhabditis elegans to explore the toxic effects of RDX on the parental and offspring worms and the involved signaling pathways. Exposure up to 1000 ng/mL of RDX produced a significant increase in reactive oxygen species (ROS) production, germ cell apoptosis, and decrease in eggs laid. Various mutants were used to demonstrate the RDX-induced apoptosis signaling pathway, and the metabolism of RDX in the nematodes was found related to cytochrome P450 and GST through RNA sequencing. Exposure of parental worms to RDX produced significant reproductive toxicity in F1 and F2, but was recovered in F3 and F4. The transgenerational effects were associated with the decreased expression of met-2, spr-5, and set-2. Our findings revealed the signaling pathways related to the reproductive toxicity caused by RDX in C. elegans and their future generations, which provided the basis for further exploration of the ecological risks of energetic compounds in the environment. |
| first_indexed | 2024-03-13T06:06:25Z |
| format | Article |
| id | doaj.art-7cbdf6e10f774824b07070f30540b1ff |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-03-13T06:06:25Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-7cbdf6e10f774824b07070f30540b1ff2023-06-12T04:08:57ZengElsevierEcotoxicology and Environmental Safety0147-65132023-07-01260115074Multi-generation reproductive toxicity of RDX and the involved signal pathways in Caenorhabditis elegansLingyan Sun0Yanping Zhou1Chunyan Wang2Yaguang Nie3An Xu4Lijun Wu5Information Materials and Intelligent Sensing Laboratory of Anhui Province, Institutes of Physical Science and Information Technology, Anhui University, Hefei 230601, PR ChinaInformation Materials and Intelligent Sensing Laboratory of Anhui Province, Institutes of Physical Science and Information Technology, Anhui University, Hefei 230601, PR ChinaInformation Materials and Intelligent Sensing Laboratory of Anhui Province, Institutes of Physical Science and Information Technology, Anhui University, Hefei 230601, PR ChinaInformation Materials and Intelligent Sensing Laboratory of Anhui Province, Institutes of Physical Science and Information Technology, Anhui University, Hefei 230601, PR China; Corresponding author.Information Materials and Intelligent Sensing Laboratory of Anhui Province, Institutes of Physical Science and Information Technology, Anhui University, Hefei 230601, PR China; Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Chinese Academy of Sciences; Anhui Province Key Laboratory of Environmental Toxicology and Pollution Control Technology, High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, PR China; Corresponding author at: Information Materials and Intelligent Sensing Laboratory of Anhui Province, Institutes of Physical Science and Information Technology, Anhui University, Hefei 230601, PR China.Information Materials and Intelligent Sensing Laboratory of Anhui Province, Institutes of Physical Science and Information Technology, Anhui University, Hefei 230601, PR ChinaAs one of the most frequently used explosives, hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) can cause persistent pollution in the environment, leading to the potential ecological threat crossing the generations. In this study, we employed Caenorhabditis elegans to explore the toxic effects of RDX on the parental and offspring worms and the involved signaling pathways. Exposure up to 1000 ng/mL of RDX produced a significant increase in reactive oxygen species (ROS) production, germ cell apoptosis, and decrease in eggs laid. Various mutants were used to demonstrate the RDX-induced apoptosis signaling pathway, and the metabolism of RDX in the nematodes was found related to cytochrome P450 and GST through RNA sequencing. Exposure of parental worms to RDX produced significant reproductive toxicity in F1 and F2, but was recovered in F3 and F4. The transgenerational effects were associated with the decreased expression of met-2, spr-5, and set-2. Our findings revealed the signaling pathways related to the reproductive toxicity caused by RDX in C. elegans and their future generations, which provided the basis for further exploration of the ecological risks of energetic compounds in the environment.http://www.sciencedirect.com/science/article/pii/S014765132300578XRDXCaenorhabditis elegansTransgenerational effectsMethylationReproductive toxicity |
| spellingShingle | Lingyan Sun Yanping Zhou Chunyan Wang Yaguang Nie An Xu Lijun Wu Multi-generation reproductive toxicity of RDX and the involved signal pathways in Caenorhabditis elegans Ecotoxicology and Environmental Safety RDX Caenorhabditis elegans Transgenerational effects Methylation Reproductive toxicity |
| title | Multi-generation reproductive toxicity of RDX and the involved signal pathways in Caenorhabditis elegans |
| title_full | Multi-generation reproductive toxicity of RDX and the involved signal pathways in Caenorhabditis elegans |
| title_fullStr | Multi-generation reproductive toxicity of RDX and the involved signal pathways in Caenorhabditis elegans |
| title_full_unstemmed | Multi-generation reproductive toxicity of RDX and the involved signal pathways in Caenorhabditis elegans |
| title_short | Multi-generation reproductive toxicity of RDX and the involved signal pathways in Caenorhabditis elegans |
| title_sort | multi generation reproductive toxicity of rdx and the involved signal pathways in caenorhabditis elegans |
| topic | RDX Caenorhabditis elegans Transgenerational effects Methylation Reproductive toxicity |
| url | http://www.sciencedirect.com/science/article/pii/S014765132300578X |
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