DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHY AND SPECTROSCOPIC METHODS FOR THE ANALYSIS OF DOXOFYLLINE IN PHARMACEUTICAL DOSAGE FORMS
A high performance liquid chromatography (HPLC) and ultraviolet spectroscopic (UV) methods were developed and validated for the quantitative estimation of doxofylline (DF) in pharmaceutical dosage forms. HPLC was carried out using reversephase technique on RP-8 column with a mobile phase composed...
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada
2013-01-01
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| Series: | Indonesian Journal of Pharmacy |
| Subjects: | |
| Online Access: | http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/509/387 |
| _version_ | 1818808500449443840 |
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| author | Ethiraj Thiruvengadam Revathi Ramadoss Ganesan Vellaichamy |
| author_facet | Ethiraj Thiruvengadam Revathi Ramadoss Ganesan Vellaichamy |
| author_sort | Ethiraj Thiruvengadam |
| collection | DOAJ |
| description | A high performance liquid chromatography (HPLC) and
ultraviolet spectroscopic (UV) methods were developed and
validated for the quantitative estimation of doxofylline (DF) in
pharmaceutical dosage forms. HPLC was carried out using reversephase technique on RP-8 column with a mobile phase composed of 0.05M phosphate buffer pH 6 and acetonitrile (60:40, v/v). The mobile phase was pumped at a flow rate of 1mL/min, and detection was made at 230nm with PDA detector. UV method was performed with λ max at 270nm with apparent molar absorptive of 0.878x103 L mol-1 cm-1. Both the methods showed good linearity, recovery and precision. No spectral or chromatographic interferences from the tablet excipients were found in UV and HPLC methods. The various parameters such as linearity, precision, accuracy, specificity, and robustness, limit of detection and limit of quantization were studied according to ICH guidelines. Statistical analysis was done by student’s t-test and F-test, which showed no significant difference between the results of both methods. So the proposed methods could be applicable for routine analysis of DF and monitoring of the quality of marketed drugs. |
| first_indexed | 2024-12-18T19:42:33Z |
| format | Article |
| id | doaj.art-7cbec9524f7c4722a893671d4ca4f110 |
| institution | Directory Open Access Journal |
| issn | 2338-9427 2338-9486 |
| language | English |
| last_indexed | 2024-12-18T19:42:33Z |
| publishDate | 2013-01-01 |
| publisher | Universitas Gadjah Mada |
| record_format | Article |
| series | Indonesian Journal of Pharmacy |
| spelling | doaj.art-7cbec9524f7c4722a893671d4ca4f1102022-12-21T20:55:24ZengUniversitas Gadjah MadaIndonesian Journal of Pharmacy2338-94272338-94862013-01-012411421http://dx.doi.org/10.14499/indonesianjpharm24iss1pp14-21DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHY AND SPECTROSCOPIC METHODS FOR THE ANALYSIS OF DOXOFYLLINE IN PHARMACEUTICAL DOSAGE FORMSEthiraj Thiruvengadam0Revathi Ramadoss1Ganesan Vellaichamy2Dept of Pharmaceutics & Pharmaceutical Analysis. The Erode College of Pharmacy, Erode, Tamilnadu, 638112, IndiaDept of Pharmaceutics & Pharmaceutical Analysis. The Erode College of Pharmacy, Erode, Tamilnadu, 638112, IndiaDept of Pharmaceutics, The Erode College of Pharmacy,Erode, Tamilnadu, 638112, IndiaA high performance liquid chromatography (HPLC) and ultraviolet spectroscopic (UV) methods were developed and validated for the quantitative estimation of doxofylline (DF) in pharmaceutical dosage forms. HPLC was carried out using reversephase technique on RP-8 column with a mobile phase composed of 0.05M phosphate buffer pH 6 and acetonitrile (60:40, v/v). The mobile phase was pumped at a flow rate of 1mL/min, and detection was made at 230nm with PDA detector. UV method was performed with λ max at 270nm with apparent molar absorptive of 0.878x103 L mol-1 cm-1. Both the methods showed good linearity, recovery and precision. No spectral or chromatographic interferences from the tablet excipients were found in UV and HPLC methods. The various parameters such as linearity, precision, accuracy, specificity, and robustness, limit of detection and limit of quantization were studied according to ICH guidelines. Statistical analysis was done by student’s t-test and F-test, which showed no significant difference between the results of both methods. So the proposed methods could be applicable for routine analysis of DF and monitoring of the quality of marketed drugs.http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/509/387DoxofyllineValidationHPLCUV spectroscopyComparison studiesStudent’s t-testF-test. |
| spellingShingle | Ethiraj Thiruvengadam Revathi Ramadoss Ganesan Vellaichamy DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHY AND SPECTROSCOPIC METHODS FOR THE ANALYSIS OF DOXOFYLLINE IN PHARMACEUTICAL DOSAGE FORMS Indonesian Journal of Pharmacy Doxofylline Validation HPLC UV spectroscopy Comparison studies Student’s t-test F-test. |
| title | DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHY AND SPECTROSCOPIC METHODS FOR THE ANALYSIS OF DOXOFYLLINE IN PHARMACEUTICAL DOSAGE FORMS |
| title_full | DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHY AND SPECTROSCOPIC METHODS FOR THE ANALYSIS OF DOXOFYLLINE IN PHARMACEUTICAL DOSAGE FORMS |
| title_fullStr | DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHY AND SPECTROSCOPIC METHODS FOR THE ANALYSIS OF DOXOFYLLINE IN PHARMACEUTICAL DOSAGE FORMS |
| title_full_unstemmed | DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHY AND SPECTROSCOPIC METHODS FOR THE ANALYSIS OF DOXOFYLLINE IN PHARMACEUTICAL DOSAGE FORMS |
| title_short | DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHY AND SPECTROSCOPIC METHODS FOR THE ANALYSIS OF DOXOFYLLINE IN PHARMACEUTICAL DOSAGE FORMS |
| title_sort | development and validation of liquid chromatography and spectroscopic methods for the analysis of doxofylline in pharmaceutical dosage forms |
| topic | Doxofylline Validation HPLC UV spectroscopy Comparison studies Student’s t-test F-test. |
| url | http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/509/387 |
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