Hypoglycemic Effects and Quality Marker Screening of <i>Dendrobium nobile</i> Lindl. at Different Growth Years
(1) Background: The effect of <i>Dendrobium nobile</i> Lindl. (<i>D. nobile</i>) on hyperglycemic syndrome has only been recently known for several years. Materials of <i>D. nobile</i> were always collected from the plants cultivated in various growth ages. Howeve...
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| Format: | Article |
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MDPI AG
2024-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/3/699 |
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| author | Yi Luo Da Yang Yanzhe Xu Di Wu Daopeng Tan Lin Qin Xingdong Wu Yanliu Lu Yuqi He |
| author_facet | Yi Luo Da Yang Yanzhe Xu Di Wu Daopeng Tan Lin Qin Xingdong Wu Yanliu Lu Yuqi He |
| author_sort | Yi Luo |
| collection | DOAJ |
| description | (1) Background: The effect of <i>Dendrobium nobile</i> Lindl. (<i>D. nobile</i>) on hyperglycemic syndrome has only been recently known for several years. Materials of <i>D. nobile</i> were always collected from the plants cultivated in various growth ages. However, regarding the efficacy of <i>D. nobile</i> on hyperglycemic syndrome, it was still unknown as to which cultivation age would be selected. On the other hand, with the lack of quality markers, it is difficult to control the quality of <i>D. nobile</i> to treat hyperglycemic syndrome. (2) Methods: The effects of <i>D. nobile</i> cultivated at year 1 and year 3 were checked on alloxan-induced diabetic mice while their body weight, diet, water intake, and urinary output were monitored. Moreover, levels of glycosylated serum protein and insulin were measured using Elisa kits. The constituents of <i>D. nobile</i> were identified and analyzed by using UPLC-Q/trap. Quality markers were screened out by integrating the data from UPLC-Q/trap into a network pharmacology model. (3) Results: The <i>D. nobile</i> cultivated at both year 1 and year 3 showed a significant effect on hyperglycemic syndrome at the high dosage level; however, regarding the significant level, <i>D. nobile</i> from year 1 showed the better effect. In <i>D. nobile</i>, most of the metabolites were identified as alkaloids and sesquiterpene glycosides. Alkaloids, represented by dendrobine, were enriched in <i>D. nobile</i> from year 1, while sesquiterpene glycosides were enriched in <i>D. nobile</i> from year 3. Twenty one metabolites were differentially expressed between <i>D. nobile</i> from year 1 and year 3. The aforementioned 21 metabolites were enriched to 34 therapeutic targets directly related to diabetes. (4) Conclusions: Regarding the therapy for hyperglycemic syndrome, <i>D. nobile</i> cultivated at year 1 was more recommended than that at year 3. Alkaloids were recommended to be used as markers to control the quality of <i>D. nobile</i> for hyperglycemic syndrome treatment. |
| first_indexed | 2024-03-08T03:51:49Z |
| format | Article |
| id | doaj.art-7cc0f3b0cb5a45cdb327f4911670724d |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-08T03:51:49Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj.art-7cc0f3b0cb5a45cdb327f4911670724d2024-02-09T15:19:08ZengMDPI AGMolecules1420-30492024-02-0129369910.3390/molecules29030699Hypoglycemic Effects and Quality Marker Screening of <i>Dendrobium nobile</i> Lindl. at Different Growth YearsYi Luo0Da Yang1Yanzhe Xu2Di Wu3Daopeng Tan4Lin Qin5Xingdong Wu6Yanliu Lu7Yuqi He8Key Lab of the Basic Pharmacology of The Ministry of Education, Zunyi Medical University, 6 West Xue-Fu Road, Zunyi 563009, ChinaKey Lab of the Basic Pharmacology of The Ministry of Education, Zunyi Medical University, 6 West Xue-Fu Road, Zunyi 563009, ChinaKey Lab of the Basic Pharmacology of The Ministry of Education, Zunyi Medical University, 6 West Xue-Fu Road, Zunyi 563009, ChinaGuizhou Engineering Research Center of Industrial Key-Technology for Dendrobium Nobile, Zunyi Medical University, 6 West Xue-Fu Road, Zunyi 563009, ChinaGuizhou Engineering Research Center of Industrial Key-Technology for Dendrobium Nobile, Zunyi Medical University, 6 West Xue-Fu Road, Zunyi 563009, ChinaGuizhou Engineering Research Center of Industrial Key-Technology for Dendrobium Nobile, Zunyi Medical University, 6 West Xue-Fu Road, Zunyi 563009, ChinaGuizhou Engineering Research Center of Industrial Key-Technology for Dendrobium Nobile, Zunyi Medical University, 6 West Xue-Fu Road, Zunyi 563009, ChinaKey Lab of the Basic Pharmacology of The Ministry of Education, Zunyi Medical University, 6 West Xue-Fu Road, Zunyi 563009, ChinaKey Lab of the Basic Pharmacology of The Ministry of Education, Zunyi Medical University, 6 West Xue-Fu Road, Zunyi 563009, China(1) Background: The effect of <i>Dendrobium nobile</i> Lindl. (<i>D. nobile</i>) on hyperglycemic syndrome has only been recently known for several years. Materials of <i>D. nobile</i> were always collected from the plants cultivated in various growth ages. However, regarding the efficacy of <i>D. nobile</i> on hyperglycemic syndrome, it was still unknown as to which cultivation age would be selected. On the other hand, with the lack of quality markers, it is difficult to control the quality of <i>D. nobile</i> to treat hyperglycemic syndrome. (2) Methods: The effects of <i>D. nobile</i> cultivated at year 1 and year 3 were checked on alloxan-induced diabetic mice while their body weight, diet, water intake, and urinary output were monitored. Moreover, levels of glycosylated serum protein and insulin were measured using Elisa kits. The constituents of <i>D. nobile</i> were identified and analyzed by using UPLC-Q/trap. Quality markers were screened out by integrating the data from UPLC-Q/trap into a network pharmacology model. (3) Results: The <i>D. nobile</i> cultivated at both year 1 and year 3 showed a significant effect on hyperglycemic syndrome at the high dosage level; however, regarding the significant level, <i>D. nobile</i> from year 1 showed the better effect. In <i>D. nobile</i>, most of the metabolites were identified as alkaloids and sesquiterpene glycosides. Alkaloids, represented by dendrobine, were enriched in <i>D. nobile</i> from year 1, while sesquiterpene glycosides were enriched in <i>D. nobile</i> from year 3. Twenty one metabolites were differentially expressed between <i>D. nobile</i> from year 1 and year 3. The aforementioned 21 metabolites were enriched to 34 therapeutic targets directly related to diabetes. (4) Conclusions: Regarding the therapy for hyperglycemic syndrome, <i>D. nobile</i> cultivated at year 1 was more recommended than that at year 3. Alkaloids were recommended to be used as markers to control the quality of <i>D. nobile</i> for hyperglycemic syndrome treatment.https://www.mdpi.com/1420-3049/29/3/699<i>Dendrobium nobile</i> Lindl.growth yeardiabeticquality markers |
| spellingShingle | Yi Luo Da Yang Yanzhe Xu Di Wu Daopeng Tan Lin Qin Xingdong Wu Yanliu Lu Yuqi He Hypoglycemic Effects and Quality Marker Screening of <i>Dendrobium nobile</i> Lindl. at Different Growth Years Molecules <i>Dendrobium nobile</i> Lindl. growth year diabetic quality markers |
| title | Hypoglycemic Effects and Quality Marker Screening of <i>Dendrobium nobile</i> Lindl. at Different Growth Years |
| title_full | Hypoglycemic Effects and Quality Marker Screening of <i>Dendrobium nobile</i> Lindl. at Different Growth Years |
| title_fullStr | Hypoglycemic Effects and Quality Marker Screening of <i>Dendrobium nobile</i> Lindl. at Different Growth Years |
| title_full_unstemmed | Hypoglycemic Effects and Quality Marker Screening of <i>Dendrobium nobile</i> Lindl. at Different Growth Years |
| title_short | Hypoglycemic Effects and Quality Marker Screening of <i>Dendrobium nobile</i> Lindl. at Different Growth Years |
| title_sort | hypoglycemic effects and quality marker screening of i dendrobium nobile i lindl at different growth years |
| topic | <i>Dendrobium nobile</i> Lindl. growth year diabetic quality markers |
| url | https://www.mdpi.com/1420-3049/29/3/699 |
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