Developments in Negative-Strand RNA Virus Reverse Genetics
Many epidemics are caused by negative-stranded RNA viruses, leading to serious disease outbreaks that threaten human life and health. These viruses also have a significant impact on animal husbandry, resulting in substantial economic losses and jeopardizing global food security and the sustainable l...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/12/3/559 |
| _version_ | 1797239937037238272 |
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| author | Mengyi Wang Jinyan Wu Xiaoan Cao Long Xu Junhuang Wu Haiyan Ding Youjun Shang |
| author_facet | Mengyi Wang Jinyan Wu Xiaoan Cao Long Xu Junhuang Wu Haiyan Ding Youjun Shang |
| author_sort | Mengyi Wang |
| collection | DOAJ |
| description | Many epidemics are caused by negative-stranded RNA viruses, leading to serious disease outbreaks that threaten human life and health. These viruses also have a significant impact on animal husbandry, resulting in substantial economic losses and jeopardizing global food security and the sustainable livelihoods of farmers. However, the pathogenic and infection mechanism of most negative-stranded RNA viruses remain unclear. Reverse genetics systems are the most powerful tools for studying viral protein function, viral gene expression regulation, viral pathogenesis, and the generation of engineered vaccines. The reverse genetics of some negative-strand viruses have been successfully constructed, while others have not. In this review, we focus on representative viruses from the Orthomyxoviridae family (IAV), the Filoviridae family (EBOV), and the Paramyxoviridae family (PPRV) to compile and summarize the existing knowledge on reverse genetics techniques for negative-strand viruses. This will provide a theoretical foundation for developing reverse genetics techniques for some negative-strand viruses. |
| first_indexed | 2024-04-24T17:59:28Z |
| format | Article |
| id | doaj.art-7cc188319f744355ab5b93cbbe3100d4 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-04-24T17:59:28Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-7cc188319f744355ab5b93cbbe3100d42024-03-27T13:55:49ZengMDPI AGMicroorganisms2076-26072024-03-0112355910.3390/microorganisms12030559Developments in Negative-Strand RNA Virus Reverse GeneticsMengyi Wang0Jinyan Wu1Xiaoan Cao2Long Xu3Junhuang Wu4Haiyan Ding5Youjun Shang6State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, ChinaState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, ChinaState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, ChinaState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, ChinaState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, ChinaState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, ChinaState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, ChinaMany epidemics are caused by negative-stranded RNA viruses, leading to serious disease outbreaks that threaten human life and health. These viruses also have a significant impact on animal husbandry, resulting in substantial economic losses and jeopardizing global food security and the sustainable livelihoods of farmers. However, the pathogenic and infection mechanism of most negative-stranded RNA viruses remain unclear. Reverse genetics systems are the most powerful tools for studying viral protein function, viral gene expression regulation, viral pathogenesis, and the generation of engineered vaccines. The reverse genetics of some negative-strand viruses have been successfully constructed, while others have not. In this review, we focus on representative viruses from the Orthomyxoviridae family (IAV), the Filoviridae family (EBOV), and the Paramyxoviridae family (PPRV) to compile and summarize the existing knowledge on reverse genetics techniques for negative-strand viruses. This will provide a theoretical foundation for developing reverse genetics techniques for some negative-strand viruses.https://www.mdpi.com/2076-2607/12/3/559negative-strand RNA virusesreverse geneticsinfluenza A virusEbola viruspeste des petits ruminants virus |
| spellingShingle | Mengyi Wang Jinyan Wu Xiaoan Cao Long Xu Junhuang Wu Haiyan Ding Youjun Shang Developments in Negative-Strand RNA Virus Reverse Genetics Microorganisms negative-strand RNA viruses reverse genetics influenza A virus Ebola virus peste des petits ruminants virus |
| title | Developments in Negative-Strand RNA Virus Reverse Genetics |
| title_full | Developments in Negative-Strand RNA Virus Reverse Genetics |
| title_fullStr | Developments in Negative-Strand RNA Virus Reverse Genetics |
| title_full_unstemmed | Developments in Negative-Strand RNA Virus Reverse Genetics |
| title_short | Developments in Negative-Strand RNA Virus Reverse Genetics |
| title_sort | developments in negative strand rna virus reverse genetics |
| topic | negative-strand RNA viruses reverse genetics influenza A virus Ebola virus peste des petits ruminants virus |
| url | https://www.mdpi.com/2076-2607/12/3/559 |
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