Rapid remodeling observed at mid-term in-vivo study of a smart reinforced acellular vascular graft implanted on a rat model
Abstract Background The poor performance of conventional techniques used in cardiovascular disease patients requiring hemodialysis or arterial bypass grafting has prompted tissue engineers to search for clinically appropriate off-the-shelf vascular grafts. Most patients with cardiovascular disease l...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-01-01
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| Series: | Journal of Biological Engineering |
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| Online Access: | https://doi.org/10.1186/s13036-022-00313-9 |
| _version_ | 1828069569885896704 |
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| author | Francis O. Obiweluozor Mukhammad Kayumov Yujin Kwak Hwa-Jin Cho Chan-Hee Park Jun-kyu Park Yun-Jin Jeong Dong-Weon Lee Do-Wan Kim In-Seok Jeong |
| author_facet | Francis O. Obiweluozor Mukhammad Kayumov Yujin Kwak Hwa-Jin Cho Chan-Hee Park Jun-kyu Park Yun-Jin Jeong Dong-Weon Lee Do-Wan Kim In-Seok Jeong |
| author_sort | Francis O. Obiweluozor |
| collection | DOAJ |
| description | Abstract Background The poor performance of conventional techniques used in cardiovascular disease patients requiring hemodialysis or arterial bypass grafting has prompted tissue engineers to search for clinically appropriate off-the-shelf vascular grafts. Most patients with cardiovascular disease lack suitable autologous tissue because of age or previous surgery. Commercially available vascular grafts with diameters of < 5 mm often fail because of thrombosis and intimal hyperplasia. Result Here, we tested tubular biodegradable poly-e-caprolactone/polydioxanone (PCL/PDO) electrospun vascular grafts in a rat model of aortic interposition for up to 12 weeks. The grafts demonstrated excellent patency (100%) confirmed by Doppler Ultrasound, resisted aneurysmal dilation and intimal hyperplasia, and yielded neoarteries largely free of foreign materials. At 12 weeks, the grafts resembled native arteries with confluent endothelium, synchronous pulsation, a contractile smooth muscle layer, and co-expression of various extracellular matrix components (elastin, collagen, and glycosaminoglycan). Conclusions The structural and functional properties comparable to native vessels observed in the neoartery indicate their potential application as an alternative for the replacement of damaged small-diameter grafts. This synthetic off-the-shelf device may be suitable for patients without autologous vessels. However, for clinical application of these grafts, long-term studies (> 1.5 years) in large animals with a vasculature similar to humans are needed. |
| first_indexed | 2024-04-11T00:21:57Z |
| format | Article |
| id | doaj.art-7cc2207ac2e94d8c85e6afd14f759f0c |
| institution | Directory Open Access Journal |
| issn | 1754-1611 |
| language | English |
| last_indexed | 2024-04-11T00:21:57Z |
| publishDate | 2023-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Biological Engineering |
| spelling | doaj.art-7cc2207ac2e94d8c85e6afd14f759f0c2023-01-08T12:14:01ZengBMCJournal of Biological Engineering1754-16112023-01-0117111310.1186/s13036-022-00313-9Rapid remodeling observed at mid-term in-vivo study of a smart reinforced acellular vascular graft implanted on a rat modelFrancis O. Obiweluozor0Mukhammad Kayumov1Yujin Kwak2Hwa-Jin Cho3Chan-Hee Park4Jun-kyu Park5Yun-Jin Jeong6Dong-Weon Lee7Do-Wan Kim8In-Seok Jeong9Research and Business Development foundation, Chonnam National UniversityDepartment of Thoracic and Cardiovascular Surgery, Chonnam National University Hospital and Medical SchoolDepartment of Thoracic and Cardiovascular Surgery, Chonnam National University Hospital and Medical SchoolDepartment of Pediatrics, Chonnam National University Children’s Hospital and Medical SchoolDepartment of Mechanical Engineering Graduate School, Chonbuk National UniversityCGBio Co. Ltd.School of Mechanical Engineering Chonnam National UniversitySchool of Mechanical Engineering Chonnam National UniversityDepartment of Thoracic and Cardiovascular Surgery, Chonnam National University Hospital and Medical SchoolDepartment of Thoracic and Cardiovascular Surgery, Chonnam National University Hospital and Medical SchoolAbstract Background The poor performance of conventional techniques used in cardiovascular disease patients requiring hemodialysis or arterial bypass grafting has prompted tissue engineers to search for clinically appropriate off-the-shelf vascular grafts. Most patients with cardiovascular disease lack suitable autologous tissue because of age or previous surgery. Commercially available vascular grafts with diameters of < 5 mm often fail because of thrombosis and intimal hyperplasia. Result Here, we tested tubular biodegradable poly-e-caprolactone/polydioxanone (PCL/PDO) electrospun vascular grafts in a rat model of aortic interposition for up to 12 weeks. The grafts demonstrated excellent patency (100%) confirmed by Doppler Ultrasound, resisted aneurysmal dilation and intimal hyperplasia, and yielded neoarteries largely free of foreign materials. At 12 weeks, the grafts resembled native arteries with confluent endothelium, synchronous pulsation, a contractile smooth muscle layer, and co-expression of various extracellular matrix components (elastin, collagen, and glycosaminoglycan). Conclusions The structural and functional properties comparable to native vessels observed in the neoartery indicate their potential application as an alternative for the replacement of damaged small-diameter grafts. This synthetic off-the-shelf device may be suitable for patients without autologous vessels. However, for clinical application of these grafts, long-term studies (> 1.5 years) in large animals with a vasculature similar to humans are needed.https://doi.org/10.1186/s13036-022-00313-9Vascular graftElectrospinningNanofibersMedium-term performanceTissue regeneration3D printing |
| spellingShingle | Francis O. Obiweluozor Mukhammad Kayumov Yujin Kwak Hwa-Jin Cho Chan-Hee Park Jun-kyu Park Yun-Jin Jeong Dong-Weon Lee Do-Wan Kim In-Seok Jeong Rapid remodeling observed at mid-term in-vivo study of a smart reinforced acellular vascular graft implanted on a rat model Journal of Biological Engineering Vascular graft Electrospinning Nanofibers Medium-term performance Tissue regeneration 3D printing |
| title | Rapid remodeling observed at mid-term in-vivo study of a smart reinforced acellular vascular graft implanted on a rat model |
| title_full | Rapid remodeling observed at mid-term in-vivo study of a smart reinforced acellular vascular graft implanted on a rat model |
| title_fullStr | Rapid remodeling observed at mid-term in-vivo study of a smart reinforced acellular vascular graft implanted on a rat model |
| title_full_unstemmed | Rapid remodeling observed at mid-term in-vivo study of a smart reinforced acellular vascular graft implanted on a rat model |
| title_short | Rapid remodeling observed at mid-term in-vivo study of a smart reinforced acellular vascular graft implanted on a rat model |
| title_sort | rapid remodeling observed at mid term in vivo study of a smart reinforced acellular vascular graft implanted on a rat model |
| topic | Vascular graft Electrospinning Nanofibers Medium-term performance Tissue regeneration 3D printing |
| url | https://doi.org/10.1186/s13036-022-00313-9 |
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