Highly efficient production of 2,3-butanediol from xylose and glucose by newly isolated thermotolerant Cronobacter sakazakii
Abstract Background 2,3-Butanediol (2,3-BD), a valuable compound used for chemicals, cosmetics, pesticides and pharmaceuticals, has been produced by various microbes. However, no high-temperature fermentation of the compound at high productivity has been reported. Methods Thermotolerant xylose-utili...
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| Format: | Article |
| Language: | English |
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BMC
2022-06-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-022-02577-z |
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| author | Chansom Keo-oudone Koudkeo Phommachan Orathai Suliya Mochamad Nurcholis Somchanh Bounphanmy Tomoyuki Kosaka Mamoru Yamada |
| author_facet | Chansom Keo-oudone Koudkeo Phommachan Orathai Suliya Mochamad Nurcholis Somchanh Bounphanmy Tomoyuki Kosaka Mamoru Yamada |
| author_sort | Chansom Keo-oudone |
| collection | DOAJ |
| description | Abstract Background 2,3-Butanediol (2,3-BD), a valuable compound used for chemicals, cosmetics, pesticides and pharmaceuticals, has been produced by various microbes. However, no high-temperature fermentation of the compound at high productivity has been reported. Methods Thermotolerant xylose-utilizing microbes were isolated from 6 different districts in Laos and screened for a low accumulation of xylitol in a xylose medium at 37 ˚C. One isolate was found to produce 2,3-BD and identified by 16S rDNA sequencing. The 2,3-BD fermentation capacity was investigated at different temperatures using xylose and glucose as carbon sources, and the fermentation parameters were determined by a high-performance liquid chromatography system. Results By screening for a low accumulation of xylitol in a xylose medium, one isolate that accumulated almost no xylitol was obtained. Further analyses revealed that the isolate is Cronobacter sakazakii and that it has the ability to produce 2,3-BD at high temperatures. When xylose and glucose were used, this strain, named C. sakazakii OX-25, accumulated 2,3-BD in a short period before the complete consumption of these sugars and then appeared to convert 2,3-BD to acetoin. The optimum temperature of the 2,3-BD fermentation was 42 ˚C to 45 ˚C, and the maximum yield of 2,3-BD was 0.3 g/g at 12 h in 20 g/l xylose medium and 0.4 g/g at 6 h in 20 g/l glucose medium at 42 ˚C. The 2,3-BD productivity of the strain was higher than the 2,3-BD productivities of other non-genetically engineered microorganisms reported previously, and the highest productivity was 0.6 g/l·h and 1.2 g/l·h for xylose and glucose, respectively. Conclusions Among thermotolerant microbes isolated in Laos, we discovered a strain, C. sakazakii OX-25, that can convert xylose and glucose to 2,3-BD with high efficiency and high productivity at high temperatures, suggesting that C. sakazakii OX-25 has the potential for industrial application to produce 2,3-BD as an important platform chemical. |
| first_indexed | 2024-12-12T07:45:52Z |
| format | Article |
| id | doaj.art-7ccaff40a9bb42eab8ebf2922f527e29 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-12T07:45:52Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
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| series | BMC Microbiology |
| spelling | doaj.art-7ccaff40a9bb42eab8ebf2922f527e292022-12-22T00:32:35ZengBMCBMC Microbiology1471-21802022-06-0122111010.1186/s12866-022-02577-zHighly efficient production of 2,3-butanediol from xylose and glucose by newly isolated thermotolerant Cronobacter sakazakiiChansom Keo-oudone0Koudkeo Phommachan1Orathai Suliya2Mochamad Nurcholis3Somchanh Bounphanmy4Tomoyuki Kosaka5Mamoru Yamada6Department of Biology, Faculty of Natural Science, National University of Laos, Lao PDRGraduate School of Sciences and Technology for Innovation, Yamaguchi UniversityDepartment of Biology, Faculty of Natural Science, National University of Laos, Lao PDRDepartment of Food Science and Technology, Faculty of Agricultural Technology, Brawijaya UniversityDepartment of Biology, Faculty of Natural Science, National University of Laos, Lao PDRGraduate School of Sciences and Technology for Innovation, Yamaguchi UniversityGraduate School of Sciences and Technology for Innovation, Yamaguchi UniversityAbstract Background 2,3-Butanediol (2,3-BD), a valuable compound used for chemicals, cosmetics, pesticides and pharmaceuticals, has been produced by various microbes. However, no high-temperature fermentation of the compound at high productivity has been reported. Methods Thermotolerant xylose-utilizing microbes were isolated from 6 different districts in Laos and screened for a low accumulation of xylitol in a xylose medium at 37 ˚C. One isolate was found to produce 2,3-BD and identified by 16S rDNA sequencing. The 2,3-BD fermentation capacity was investigated at different temperatures using xylose and glucose as carbon sources, and the fermentation parameters were determined by a high-performance liquid chromatography system. Results By screening for a low accumulation of xylitol in a xylose medium, one isolate that accumulated almost no xylitol was obtained. Further analyses revealed that the isolate is Cronobacter sakazakii and that it has the ability to produce 2,3-BD at high temperatures. When xylose and glucose were used, this strain, named C. sakazakii OX-25, accumulated 2,3-BD in a short period before the complete consumption of these sugars and then appeared to convert 2,3-BD to acetoin. The optimum temperature of the 2,3-BD fermentation was 42 ˚C to 45 ˚C, and the maximum yield of 2,3-BD was 0.3 g/g at 12 h in 20 g/l xylose medium and 0.4 g/g at 6 h in 20 g/l glucose medium at 42 ˚C. The 2,3-BD productivity of the strain was higher than the 2,3-BD productivities of other non-genetically engineered microorganisms reported previously, and the highest productivity was 0.6 g/l·h and 1.2 g/l·h for xylose and glucose, respectively. Conclusions Among thermotolerant microbes isolated in Laos, we discovered a strain, C. sakazakii OX-25, that can convert xylose and glucose to 2,3-BD with high efficiency and high productivity at high temperatures, suggesting that C. sakazakii OX-25 has the potential for industrial application to produce 2,3-BD as an important platform chemical.https://doi.org/10.1186/s12866-022-02577-z2,3-ButanediolCronobacter sakazakiiXylose-fermenting microbeHigh-temperature fermentationLignocellulosic biomass |
| spellingShingle | Chansom Keo-oudone Koudkeo Phommachan Orathai Suliya Mochamad Nurcholis Somchanh Bounphanmy Tomoyuki Kosaka Mamoru Yamada Highly efficient production of 2,3-butanediol from xylose and glucose by newly isolated thermotolerant Cronobacter sakazakii BMC Microbiology 2,3-Butanediol Cronobacter sakazakii Xylose-fermenting microbe High-temperature fermentation Lignocellulosic biomass |
| title | Highly efficient production of 2,3-butanediol from xylose and glucose by newly isolated thermotolerant Cronobacter sakazakii |
| title_full | Highly efficient production of 2,3-butanediol from xylose and glucose by newly isolated thermotolerant Cronobacter sakazakii |
| title_fullStr | Highly efficient production of 2,3-butanediol from xylose and glucose by newly isolated thermotolerant Cronobacter sakazakii |
| title_full_unstemmed | Highly efficient production of 2,3-butanediol from xylose and glucose by newly isolated thermotolerant Cronobacter sakazakii |
| title_short | Highly efficient production of 2,3-butanediol from xylose and glucose by newly isolated thermotolerant Cronobacter sakazakii |
| title_sort | highly efficient production of 2 3 butanediol from xylose and glucose by newly isolated thermotolerant cronobacter sakazakii |
| topic | 2,3-Butanediol Cronobacter sakazakii Xylose-fermenting microbe High-temperature fermentation Lignocellulosic biomass |
| url | https://doi.org/10.1186/s12866-022-02577-z |
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