Dietary long-chain n-3 PUFAs increase LPL gene expression in adipose tissue of subjects with an atherogenic lipoprotein phenotype
We sought to test the hypothesis that dietary long-chain n-3 PUFA (LC n-3 PUFA) in fish oil stimulate the gene expression of lipoprotein lipase (LPL) in human adipose tissue (AT). In a randomized, double blind, placebo-controlled, cross-over study, 51 male subjects expressing an atherogenic lipoprot...
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| Format: | Article |
| Language: | English |
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Elsevier
2002-06-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520304739 |
| _version_ | 1818931389343465472 |
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| author | Syrah Khan Anne-Marie Minihane Philippa J. Talmud John W. Wright Margaret C. Murphy Christine M. Williams Bruce A. Griffin |
| author_facet | Syrah Khan Anne-Marie Minihane Philippa J. Talmud John W. Wright Margaret C. Murphy Christine M. Williams Bruce A. Griffin |
| author_sort | Syrah Khan |
| collection | DOAJ |
| description | We sought to test the hypothesis that dietary long-chain n-3 PUFA (LC n-3 PUFA) in fish oil stimulate the gene expression of lipoprotein lipase (LPL) in human adipose tissue (AT). In a randomized, double blind, placebo-controlled, cross-over study, 51 male subjects expressing an atherogenic lipoprotein phenotype (ALP) had their diets supplemented with fish oil for 6 weeks. As we previously reported for this group, supplementation with LC n-3 PUFA produced a decrease in fasting plasma triglyceride (TG) (−35%, P < 0.05), attenuation of the postprandial TG response (area and incremental area under the curve; AUC and IAUC, P < 0.05), and a decrease in small, dense LDL. The present study extended these observations by showing that these changes were accompanied by a marked increase in the concentration of LPL mRNA in adipose tissue (AT-LPL mRNA, +55%, P = 0.003) and post-heparin LPL activity (PH-LPL, +31%, P = 0.036). There was also evidence of an association between LPL gene expression and polymorphism in the apolipoprotein E gene.We conclude that the favorable influence of dietary n-3 PUFA on the ALP may be mediated, in part, through an increase in the plasma activity and gene expression of lipoprotein lipase in human adipose tissue. |
| first_indexed | 2024-12-20T04:15:49Z |
| format | Article |
| id | doaj.art-7ccce47a49f4415cb4a49998362b7c0a |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T04:15:49Z |
| publishDate | 2002-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-7ccce47a49f4415cb4a49998362b7c0a2022-12-21T19:53:47ZengElsevierJournal of Lipid Research0022-22752002-06-01436979985Dietary long-chain n-3 PUFAs increase LPL gene expression in adipose tissue of subjects with an atherogenic lipoprotein phenotypeSyrah Khan0Anne-Marie Minihane1Philippa J. Talmud2John W. Wright3Margaret C. Murphy4Christine M. Williams5Bruce A. Griffin6School of Biomedical & Life Sciences, University of Surrey, Guildford, United KingdomSchool of Food Biosciences, University of Reading, Reading, United KingdomCentre of Cardiovascular Genetics, University College London, London, United KingdomSchool of Biomedical & Life Sciences, University of Surrey, Guildford, United KingdomEuropean Institute of Health & Medical Sciences, University of Surrey, Guildford, United KingdomSchool of Food Biosciences, University of Reading, Reading, United KingdomSchool of Biomedical & Life Sciences, University of Surrey, Guildford, United KingdomWe sought to test the hypothesis that dietary long-chain n-3 PUFA (LC n-3 PUFA) in fish oil stimulate the gene expression of lipoprotein lipase (LPL) in human adipose tissue (AT). In a randomized, double blind, placebo-controlled, cross-over study, 51 male subjects expressing an atherogenic lipoprotein phenotype (ALP) had their diets supplemented with fish oil for 6 weeks. As we previously reported for this group, supplementation with LC n-3 PUFA produced a decrease in fasting plasma triglyceride (TG) (−35%, P < 0.05), attenuation of the postprandial TG response (area and incremental area under the curve; AUC and IAUC, P < 0.05), and a decrease in small, dense LDL. The present study extended these observations by showing that these changes were accompanied by a marked increase in the concentration of LPL mRNA in adipose tissue (AT-LPL mRNA, +55%, P = 0.003) and post-heparin LPL activity (PH-LPL, +31%, P = 0.036). There was also evidence of an association between LPL gene expression and polymorphism in the apolipoprotein E gene.We conclude that the favorable influence of dietary n-3 PUFA on the ALP may be mediated, in part, through an increase in the plasma activity and gene expression of lipoprotein lipase in human adipose tissue.http://www.sciencedirect.com/science/article/pii/S0022227520304739polyunsaturated fatty acidslipoprotein lipase genefish oil |
| spellingShingle | Syrah Khan Anne-Marie Minihane Philippa J. Talmud John W. Wright Margaret C. Murphy Christine M. Williams Bruce A. Griffin Dietary long-chain n-3 PUFAs increase LPL gene expression in adipose tissue of subjects with an atherogenic lipoprotein phenotype Journal of Lipid Research polyunsaturated fatty acids lipoprotein lipase gene fish oil |
| title | Dietary long-chain n-3 PUFAs increase LPL gene expression in adipose tissue of subjects with an atherogenic lipoprotein phenotype |
| title_full | Dietary long-chain n-3 PUFAs increase LPL gene expression in adipose tissue of subjects with an atherogenic lipoprotein phenotype |
| title_fullStr | Dietary long-chain n-3 PUFAs increase LPL gene expression in adipose tissue of subjects with an atherogenic lipoprotein phenotype |
| title_full_unstemmed | Dietary long-chain n-3 PUFAs increase LPL gene expression in adipose tissue of subjects with an atherogenic lipoprotein phenotype |
| title_short | Dietary long-chain n-3 PUFAs increase LPL gene expression in adipose tissue of subjects with an atherogenic lipoprotein phenotype |
| title_sort | dietary long chain n 3 pufas increase lpl gene expression in adipose tissue of subjects with an atherogenic lipoprotein phenotype |
| topic | polyunsaturated fatty acids lipoprotein lipase gene fish oil |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520304739 |
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