Hypothalamic Expression of Neuropeptide Y (NPY) and Pro-OpioMelanoCortin (POMC) in Adult Male Mice Is Affected by Chronic Exposure to Endocrine Disruptors
In the arcuate nucleus, neuropeptide Y (NPY) neurons, increase food intake and decrease energy expenditure, and control the activity of pro-opiomelanocortin (POMC) neurons, that decrease food intake and increase energy expenditure. Both systems project to other hypothalamic nuclei such as the parave...
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| author | Marilena Marraudino Elisabetta Bo Elisabetta Carlini Alice Farinetti Giovanna Ponti Isabella Zanella Diego Di Lorenzo Gian Carlo Panzica Stefano Gotti |
| author_facet | Marilena Marraudino Elisabetta Bo Elisabetta Carlini Alice Farinetti Giovanna Ponti Isabella Zanella Diego Di Lorenzo Gian Carlo Panzica Stefano Gotti |
| author_sort | Marilena Marraudino |
| collection | DOAJ |
| description | In the arcuate nucleus, neuropeptide Y (NPY) neurons, increase food intake and decrease energy expenditure, and control the activity of pro-opiomelanocortin (POMC) neurons, that decrease food intake and increase energy expenditure. Both systems project to other hypothalamic nuclei such as the paraventricular and dorsomedial hypothalamic nuclei. Endocrine disrupting chemicals (EDCs) are environmental contaminants that alter the endocrine system causing adverse health effects in an intact organism or its progeny. We investigated the effects of long-term exposure to some EDCs on the hypothalamic NPY and POMC systems of adult male mice that had been previously demonstrated to be a target of some of these EDCs after short-term exposure. Animals were chronically fed for four months with a phytoestrogen-free diet containing two different concentrations of bisphenol A, diethylstilbestrol, tributyltin, or E<sub>2</sub>. At the end, brains were processed for NPY and POMC immunohistochemistry and quantitatively analyzed. In the arcuate and dorsomedial nuclei, both NPY and POMC immunoreactivity showed a statistically significant decrease. In the paraventricular nucleus, only the NPY system was affected, while the POMC system was not affected. Finally, in the VMH the NPY system was affected whereas no POMC immunoreactive material was observed. These results indicate that adult exposure to different EDCs may alter the hypothalamic circuits that control food intake and energy metabolism. |
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| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T10:34:14Z |
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| series | Metabolites |
| spelling | doaj.art-7cd0627be9b045979c7f1d9f9a7946822023-11-21T23:23:56ZengMDPI AGMetabolites2218-19892021-06-0111636810.3390/metabo11060368Hypothalamic Expression of Neuropeptide Y (NPY) and Pro-OpioMelanoCortin (POMC) in Adult Male Mice Is Affected by Chronic Exposure to Endocrine DisruptorsMarilena Marraudino0Elisabetta Bo1Elisabetta Carlini2Alice Farinetti3Giovanna Ponti4Isabella Zanella5Diego Di Lorenzo6Gian Carlo Panzica7Stefano Gotti8Laboratory of Neuroendocrinology, Department of Neuroscience, University of Torino, 10126 Torino, ItalyLaboratory of Neuroendocrinology, Department of Neuroscience, University of Torino, 10126 Torino, ItalyLaboratory of Neuroendocrinology, Department of Neuroscience, University of Torino, 10126 Torino, ItalyLaboratory of Neuroendocrinology, Department of Neuroscience, University of Torino, 10126 Torino, ItalyNeuroscience Institute Cavalieri-Ottolenghi (NICO), 10043 Orbassano, ItalyDepartment of Molecular and Translational Medicine, University of Brescia, 25121 Brescia, ItalyClinical Chemistry Laboratory, Diagnostic Department, ASST Spedali Civili di Brescia, 25123 Brescia, ItalyLaboratory of Neuroendocrinology, Department of Neuroscience, University of Torino, 10126 Torino, ItalyLaboratory of Neuroendocrinology, Department of Neuroscience, University of Torino, 10126 Torino, ItalyIn the arcuate nucleus, neuropeptide Y (NPY) neurons, increase food intake and decrease energy expenditure, and control the activity of pro-opiomelanocortin (POMC) neurons, that decrease food intake and increase energy expenditure. Both systems project to other hypothalamic nuclei such as the paraventricular and dorsomedial hypothalamic nuclei. Endocrine disrupting chemicals (EDCs) are environmental contaminants that alter the endocrine system causing adverse health effects in an intact organism or its progeny. We investigated the effects of long-term exposure to some EDCs on the hypothalamic NPY and POMC systems of adult male mice that had been previously demonstrated to be a target of some of these EDCs after short-term exposure. Animals were chronically fed for four months with a phytoestrogen-free diet containing two different concentrations of bisphenol A, diethylstilbestrol, tributyltin, or E<sub>2</sub>. At the end, brains were processed for NPY and POMC immunohistochemistry and quantitatively analyzed. In the arcuate and dorsomedial nuclei, both NPY and POMC immunoreactivity showed a statistically significant decrease. In the paraventricular nucleus, only the NPY system was affected, while the POMC system was not affected. Finally, in the VMH the NPY system was affected whereas no POMC immunoreactive material was observed. These results indicate that adult exposure to different EDCs may alter the hypothalamic circuits that control food intake and energy metabolism.https://www.mdpi.com/2218-1989/11/6/368endocrine disrupting chemicalsbisphenol Adiethylstilbestroltributyltinneuropeptide Ypro-opiomelanocortin |
| spellingShingle | Marilena Marraudino Elisabetta Bo Elisabetta Carlini Alice Farinetti Giovanna Ponti Isabella Zanella Diego Di Lorenzo Gian Carlo Panzica Stefano Gotti Hypothalamic Expression of Neuropeptide Y (NPY) and Pro-OpioMelanoCortin (POMC) in Adult Male Mice Is Affected by Chronic Exposure to Endocrine Disruptors Metabolites endocrine disrupting chemicals bisphenol A diethylstilbestrol tributyltin neuropeptide Y pro-opiomelanocortin |
| title | Hypothalamic Expression of Neuropeptide Y (NPY) and Pro-OpioMelanoCortin (POMC) in Adult Male Mice Is Affected by Chronic Exposure to Endocrine Disruptors |
| title_full | Hypothalamic Expression of Neuropeptide Y (NPY) and Pro-OpioMelanoCortin (POMC) in Adult Male Mice Is Affected by Chronic Exposure to Endocrine Disruptors |
| title_fullStr | Hypothalamic Expression of Neuropeptide Y (NPY) and Pro-OpioMelanoCortin (POMC) in Adult Male Mice Is Affected by Chronic Exposure to Endocrine Disruptors |
| title_full_unstemmed | Hypothalamic Expression of Neuropeptide Y (NPY) and Pro-OpioMelanoCortin (POMC) in Adult Male Mice Is Affected by Chronic Exposure to Endocrine Disruptors |
| title_short | Hypothalamic Expression of Neuropeptide Y (NPY) and Pro-OpioMelanoCortin (POMC) in Adult Male Mice Is Affected by Chronic Exposure to Endocrine Disruptors |
| title_sort | hypothalamic expression of neuropeptide y npy and pro opiomelanocortin pomc in adult male mice is affected by chronic exposure to endocrine disruptors |
| topic | endocrine disrupting chemicals bisphenol A diethylstilbestrol tributyltin neuropeptide Y pro-opiomelanocortin |
| url | https://www.mdpi.com/2218-1989/11/6/368 |
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