Effects of Permeable Cryoprotectants on Viability of Mammalian Embryo Mo
The objective of this study was to evaluate the toxicities of permeable cryoprotectants and finally to establish the cryopreservation method of surplus embryos obtained during assisted reproductive technology (ART). Toxicities of permeable cryoprotectants, dimethyl sulfoxide (DMSO), ethylene glycol...
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| Format: | Article |
| Language: | English |
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The Korean Society of Animal Reproduction and Biotechnology
2015-09-01
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| Series: | Journal of Animal Reproduction and Biotechnology |
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| Online Access: | http://www.e-jarb.org/journal/view.html?uid=381&vmd=Full |
| _version_ | 1818725655366336512 |
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| author | Hyun Kim Sang-Rae Cho Dong Kyo Kim Changyong Choe Hwan-Hoo Seong |
| author_facet | Hyun Kim Sang-Rae Cho Dong Kyo Kim Changyong Choe Hwan-Hoo Seong |
| author_sort | Hyun Kim |
| collection | DOAJ |
| description | The objective of this study was to evaluate the toxicities of permeable cryoprotectants and finally to establish the cryopreservation method of surplus embryos obtained during assisted reproductive technology (ART). Toxicities of permeable cryoprotectants, dimethyl sulfoxide (DMSO), ethylene glycol (EG), Glycerol, and 1,2-PROH were investigated using a murine embryo model. Female F-1 mice were stimulated with gonadotropin, induced ovulation with hCG and mated. Two cell embryos were collected and cultured after exposure to among DMSO, EG, Glycerol, and 1,2-PROH. Embryo development was evaluated up to the blastocyst stage. The total cell count of blastocysts that were treated with DMSO and Glycerol at the 2-cell stage was significantly lower than that were treated with EG (81.1±15.1), 1,2-PROH (88.0±21.1) or the control (99.9±21.3) (p<0.001). On comparison of four cryoprotectant treated groups, the DMSO and Glycerol treated group showed a decreased cell count compared with the EG and 1,2-PROH treated group (p<0.05). Both DMSO (14.7±1.3), EG (12.1±1.1), Glycerol (15.2±1.8), and 1,2-PROH (11.5±1.3) treated groups showed higher apoptosis rates of cells in the blastocyst compared with the control (6.5±0.7, p<0.0001). In addition, the DMSO or Glycerol treated group showed more apoptotic cells than the EG or 1,2-PROH treated group (p<0.001). The potential toxicity of cryoprotectants was uncovered by prolonged exposure of murine embryos to among DMSO, EG, Glycerol, and 1,2-PROH at room temperature. When comparing four permeable cryoprotective agents, EG and 1,2-PROH appeared to be less toxic than DMSO and Glycerol at least in a murine embryo model. |
| first_indexed | 2024-12-17T21:45:46Z |
| format | Article |
| id | doaj.art-7cd1671921324878a6339193f3d28ae3 |
| institution | Directory Open Access Journal |
| issn | 2671-4639 2671-4663 |
| language | English |
| last_indexed | 2024-12-17T21:45:46Z |
| publishDate | 2015-09-01 |
| publisher | The Korean Society of Animal Reproduction and Biotechnology |
| record_format | Article |
| series | Journal of Animal Reproduction and Biotechnology |
| spelling | doaj.art-7cd1671921324878a6339193f3d28ae32022-12-21T21:31:28ZengThe Korean Society of Animal Reproduction and BiotechnologyJournal of Animal Reproduction and Biotechnology2671-46392671-46632015-09-0130319520010.12750/JET.2015.30.3.195Effects of Permeable Cryoprotectants on Viability of Mammalian Embryo MoHyun Kim0Sang-Rae Cho1Dong Kyo Kim2Changyong Choe3Hwan-Hoo Seong4Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 590-832, KoreaHanwoo Research Station, National Institute of Animal Science, RDA, Pyeongchang 25340, KoreaAnimal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 590-832, KoreaAnimal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 590-832, KoreaAnimal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 590-832, KoreaThe objective of this study was to evaluate the toxicities of permeable cryoprotectants and finally to establish the cryopreservation method of surplus embryos obtained during assisted reproductive technology (ART). Toxicities of permeable cryoprotectants, dimethyl sulfoxide (DMSO), ethylene glycol (EG), Glycerol, and 1,2-PROH were investigated using a murine embryo model. Female F-1 mice were stimulated with gonadotropin, induced ovulation with hCG and mated. Two cell embryos were collected and cultured after exposure to among DMSO, EG, Glycerol, and 1,2-PROH. Embryo development was evaluated up to the blastocyst stage. The total cell count of blastocysts that were treated with DMSO and Glycerol at the 2-cell stage was significantly lower than that were treated with EG (81.1±15.1), 1,2-PROH (88.0±21.1) or the control (99.9±21.3) (p<0.001). On comparison of four cryoprotectant treated groups, the DMSO and Glycerol treated group showed a decreased cell count compared with the EG and 1,2-PROH treated group (p<0.05). Both DMSO (14.7±1.3), EG (12.1±1.1), Glycerol (15.2±1.8), and 1,2-PROH (11.5±1.3) treated groups showed higher apoptosis rates of cells in the blastocyst compared with the control (6.5±0.7, p<0.0001). In addition, the DMSO or Glycerol treated group showed more apoptotic cells than the EG or 1,2-PROH treated group (p<0.001). The potential toxicity of cryoprotectants was uncovered by prolonged exposure of murine embryos to among DMSO, EG, Glycerol, and 1,2-PROH at room temperature. When comparing four permeable cryoprotective agents, EG and 1,2-PROH appeared to be less toxic than DMSO and Glycerol at least in a murine embryo model.http://www.e-jarb.org/journal/view.html?uid=381&vmd=Fullcryoprotectantdmsoegtoxicityembryoapoptosis |
| spellingShingle | Hyun Kim Sang-Rae Cho Dong Kyo Kim Changyong Choe Hwan-Hoo Seong Effects of Permeable Cryoprotectants on Viability of Mammalian Embryo Mo Journal of Animal Reproduction and Biotechnology cryoprotectant dmso eg toxicity embryo apoptosis |
| title | Effects of Permeable Cryoprotectants on Viability of Mammalian Embryo Mo |
| title_full | Effects of Permeable Cryoprotectants on Viability of Mammalian Embryo Mo |
| title_fullStr | Effects of Permeable Cryoprotectants on Viability of Mammalian Embryo Mo |
| title_full_unstemmed | Effects of Permeable Cryoprotectants on Viability of Mammalian Embryo Mo |
| title_short | Effects of Permeable Cryoprotectants on Viability of Mammalian Embryo Mo |
| title_sort | effects of permeable cryoprotectants on viability of mammalian embryo mo |
| topic | cryoprotectant dmso eg toxicity embryo apoptosis |
| url | http://www.e-jarb.org/journal/view.html?uid=381&vmd=Full |
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