Ionic-microenvironment stabilizes the disulfide engineered lysine decarboxylase for efficient cadaverine production
Cadaverine is the key monomer for the synthesis of nylon 5X. Efficient and alkaline stable lysine decarboxylases are highly desirable for cadaverine production as the reaction pH increasing from 6.3 to 8.5. However, the most studied lysine decarboxylase CadA (E. coli) lost almost all activity at pH...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co. Ltd.
2023-06-01
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| Series: | Green Chemical Engineering |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666952821000881 |
| _version_ | 1827934014230495232 |
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| author | Zhuang Li Yaju Xue Xiuling Ji Yuhong Huang |
| author_facet | Zhuang Li Yaju Xue Xiuling Ji Yuhong Huang |
| author_sort | Zhuang Li |
| collection | DOAJ |
| description | Cadaverine is the key monomer for the synthesis of nylon 5X. Efficient and alkaline stable lysine decarboxylases are highly desirable for cadaverine production as the reaction pH increasing from 6.3 to 8.5. However, the most studied lysine decarboxylase CadA (E. coli) lost almost all activity at pH 8.0, which is the foremost challenge for the industrial-cadaverine production. In this study, we first found that the Na+-microenvironment significantly improved the alkaline stability of the disulfide engineered lysine decarboxylase ΔLdcEt3 (P233C/L628C) (half-life 362 h), compared to the conventional buffer (half-life 0.66 h) at pH 8.0. Meanwhile, the whole-cell conversion efficiency of the industrial-grade l-lysine with ΔLdcEt3 could reach up to 99% in 2 h in the fermenter. Experimental investigation and molecular dynamics confirmed that Na+-microenvironment could improve active-aggregation state and affect secondary structure of ΔLdcEt3. Therefore, Na+-microenvironment stabilizes ΔLdcEt3 providing a great potential industrial application for high-level cadaverine production. |
| first_indexed | 2024-03-13T07:32:35Z |
| format | Article |
| id | doaj.art-7cd20434038248b580f5af9c90c3ca16 |
| institution | Directory Open Access Journal |
| issn | 2666-9528 |
| language | English |
| last_indexed | 2024-03-13T07:32:35Z |
| publishDate | 2023-06-01 |
| publisher | KeAi Communications Co. Ltd. |
| record_format | Article |
| series | Green Chemical Engineering |
| spelling | doaj.art-7cd20434038248b580f5af9c90c3ca162023-06-04T04:24:32ZengKeAi Communications Co. Ltd.Green Chemical Engineering2666-95282023-06-0142224232Ionic-microenvironment stabilizes the disulfide engineered lysine decarboxylase for efficient cadaverine productionZhuang Li0Yaju Xue1Xiuling Ji2Yuhong Huang3Beijing Key Laboratory of Ionic Liquids Clean Process, CAS Key Laboratory of Green Process and Engineering, State Key Laboratory of Multiphase Complex Systems, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China; School of Chemical Engineering, University of Chinese Academy of Sciences, Beijing, 100049, ChinaBeijing Key Laboratory of Ionic Liquids Clean Process, CAS Key Laboratory of Green Process and Engineering, State Key Laboratory of Multiphase Complex Systems, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, ChinaBeijing Key Laboratory of Ionic Liquids Clean Process, CAS Key Laboratory of Green Process and Engineering, State Key Laboratory of Multiphase Complex Systems, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, ChinaBeijing Key Laboratory of Ionic Liquids Clean Process, CAS Key Laboratory of Green Process and Engineering, State Key Laboratory of Multiphase Complex Systems, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China; Innovation Academy for Green Manufacture, Chinese Academy of Sciences, Beijing, 100190, China; Zhengzhou Institute of Emerging Industrial Technology, Zhengzhou, 450000, China; Zhongke Langfang Institute of Process Engineering, Langfang, 065001, China; Corresponding author.Cadaverine is the key monomer for the synthesis of nylon 5X. Efficient and alkaline stable lysine decarboxylases are highly desirable for cadaverine production as the reaction pH increasing from 6.3 to 8.5. However, the most studied lysine decarboxylase CadA (E. coli) lost almost all activity at pH 8.0, which is the foremost challenge for the industrial-cadaverine production. In this study, we first found that the Na+-microenvironment significantly improved the alkaline stability of the disulfide engineered lysine decarboxylase ΔLdcEt3 (P233C/L628C) (half-life 362 h), compared to the conventional buffer (half-life 0.66 h) at pH 8.0. Meanwhile, the whole-cell conversion efficiency of the industrial-grade l-lysine with ΔLdcEt3 could reach up to 99% in 2 h in the fermenter. Experimental investigation and molecular dynamics confirmed that Na+-microenvironment could improve active-aggregation state and affect secondary structure of ΔLdcEt3. Therefore, Na+-microenvironment stabilizes ΔLdcEt3 providing a great potential industrial application for high-level cadaverine production.http://www.sciencedirect.com/science/article/pii/S2666952821000881Na+-microenvironmentLysine decarboxylaseΔLdcEt3Alkaline stabilityCadaverine |
| spellingShingle | Zhuang Li Yaju Xue Xiuling Ji Yuhong Huang Ionic-microenvironment stabilizes the disulfide engineered lysine decarboxylase for efficient cadaverine production Green Chemical Engineering Na+-microenvironment Lysine decarboxylase ΔLdcEt3 Alkaline stability Cadaverine |
| title | Ionic-microenvironment stabilizes the disulfide engineered lysine decarboxylase for efficient cadaverine production |
| title_full | Ionic-microenvironment stabilizes the disulfide engineered lysine decarboxylase for efficient cadaverine production |
| title_fullStr | Ionic-microenvironment stabilizes the disulfide engineered lysine decarboxylase for efficient cadaverine production |
| title_full_unstemmed | Ionic-microenvironment stabilizes the disulfide engineered lysine decarboxylase for efficient cadaverine production |
| title_short | Ionic-microenvironment stabilizes the disulfide engineered lysine decarboxylase for efficient cadaverine production |
| title_sort | ionic microenvironment stabilizes the disulfide engineered lysine decarboxylase for efficient cadaverine production |
| topic | Na+-microenvironment Lysine decarboxylase ΔLdcEt3 Alkaline stability Cadaverine |
| url | http://www.sciencedirect.com/science/article/pii/S2666952821000881 |
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