Multi-drug resistant extended-spectrum beta-lactamase producing E. coli and Salmonella on raw vegetable salads served at hotels and restaurants in Bharatpur, Nepal
Abstract Objective Antimicrobial resistance among the bacteria present in ready-to-eat foods like vegetable salads is an emerging concern today. The current study was undertaken to investigate the presence of multi-drug resistant extended-spectrum β-lactamase (ESBL) producing E. coli and Salmonella...
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| Format: | Article |
| Language: | English |
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BMC
2019-08-01
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| Series: | BMC Research Notes |
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| Online Access: | http://link.springer.com/article/10.1186/s13104-019-4557-9 |
| _version_ | 1818254085583798272 |
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| author | Sanjeep Sapkota Sanjib Adhikari Asmita Pandey Sujan Khadka Madhuri Adhikari Hemraj Kandel Sandhya Pathak Asmita Pandey |
| author_facet | Sanjeep Sapkota Sanjib Adhikari Asmita Pandey Sujan Khadka Madhuri Adhikari Hemraj Kandel Sandhya Pathak Asmita Pandey |
| author_sort | Sanjeep Sapkota |
| collection | DOAJ |
| description | Abstract Objective Antimicrobial resistance among the bacteria present in ready-to-eat foods like vegetable salads is an emerging concern today. The current study was undertaken to investigate the presence of multi-drug resistant extended-spectrum β-lactamase (ESBL) producing E. coli and Salmonella spp. in raw vegetable salads served at hotels and restaurants in Bharatpur. A total of 216 salad samples were collected from three different grades of hotels and restaurants and examined for the presence of E. coli and Salmonella spp. in Microbiology laboratory of Birendra Multiple Campus by conventional microbiological techniques. Results Out of 216 samples, 66 samples (35.2%) showed the presence of Salmonella spp. whereas E. coli was recovered from 29 (13.4%) samples of which 3 samples harbored E. coli O157: H7. Antibiotic susceptibility testing revealed that 9 (13.6%) Salmonella and 4 (13.8%) E. coli isolates were detected as multi-drug resistant. Total ESBL producers reported were 5 (7.57%) Salmonella and 4 (13.8%) E. coli. The study also assessed a significant association between occurrence of E. coli and Salmonella with different grades of hotels and restaurants, personal hygiene and literacy rate of chefs and with the type of cleaning materials used to wash knives and chopping boards (p < 0.05). The findings suggest an immediate need of attention by the concerned authorities to prevent the emergence and transmission of food-borne pathogens and infections antimicrobial resistance among them. |
| first_indexed | 2024-12-12T16:50:22Z |
| format | Article |
| id | doaj.art-7cd204e372c64914a866baf5d9d4464e |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-12T16:50:22Z |
| publishDate | 2019-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-7cd204e372c64914a866baf5d9d4464e2022-12-22T00:18:22ZengBMCBMC Research Notes1756-05002019-08-011211610.1186/s13104-019-4557-9Multi-drug resistant extended-spectrum beta-lactamase producing E. coli and Salmonella on raw vegetable salads served at hotels and restaurants in Bharatpur, NepalSanjeep Sapkota0Sanjib Adhikari1Asmita Pandey2Sujan Khadka3Madhuri Adhikari4Hemraj Kandel5Sandhya Pathak6Asmita Pandey7Department of Microbiology, Birendra Multiple Campus, Tribhuvan UniversityDepartment of Microbiology, Birendra Multiple Campus, Tribhuvan UniversityKey Laboratory of Protein and Peptide Drugs, Institute of Biophysics, Chinese Academy of SciencesDepartment of Life Sciences, Biochemistry and Molecular Biology, Central China Normal UniversityDepartment of Microbiology, Birendra Multiple Campus, Tribhuvan UniversityDepartment of Microbiology, Birendra Multiple Campus, Tribhuvan UniversityDepartment of Microbiology, Birendra Multiple Campus, Tribhuvan UniversityDepartment of Microbiology, Birendra Multiple Campus, Tribhuvan UniversityAbstract Objective Antimicrobial resistance among the bacteria present in ready-to-eat foods like vegetable salads is an emerging concern today. The current study was undertaken to investigate the presence of multi-drug resistant extended-spectrum β-lactamase (ESBL) producing E. coli and Salmonella spp. in raw vegetable salads served at hotels and restaurants in Bharatpur. A total of 216 salad samples were collected from three different grades of hotels and restaurants and examined for the presence of E. coli and Salmonella spp. in Microbiology laboratory of Birendra Multiple Campus by conventional microbiological techniques. Results Out of 216 samples, 66 samples (35.2%) showed the presence of Salmonella spp. whereas E. coli was recovered from 29 (13.4%) samples of which 3 samples harbored E. coli O157: H7. Antibiotic susceptibility testing revealed that 9 (13.6%) Salmonella and 4 (13.8%) E. coli isolates were detected as multi-drug resistant. Total ESBL producers reported were 5 (7.57%) Salmonella and 4 (13.8%) E. coli. The study also assessed a significant association between occurrence of E. coli and Salmonella with different grades of hotels and restaurants, personal hygiene and literacy rate of chefs and with the type of cleaning materials used to wash knives and chopping boards (p < 0.05). The findings suggest an immediate need of attention by the concerned authorities to prevent the emergence and transmission of food-borne pathogens and infections antimicrobial resistance among them.http://link.springer.com/article/10.1186/s13104-019-4557-9SaladsE. coli O157: H7AntibiogramsMulti-drug resistantESBLHotels and restaurants |
| spellingShingle | Sanjeep Sapkota Sanjib Adhikari Asmita Pandey Sujan Khadka Madhuri Adhikari Hemraj Kandel Sandhya Pathak Asmita Pandey Multi-drug resistant extended-spectrum beta-lactamase producing E. coli and Salmonella on raw vegetable salads served at hotels and restaurants in Bharatpur, Nepal BMC Research Notes Salads E. coli O157: H7 Antibiograms Multi-drug resistant ESBL Hotels and restaurants |
| title | Multi-drug resistant extended-spectrum beta-lactamase producing E. coli and Salmonella on raw vegetable salads served at hotels and restaurants in Bharatpur, Nepal |
| title_full | Multi-drug resistant extended-spectrum beta-lactamase producing E. coli and Salmonella on raw vegetable salads served at hotels and restaurants in Bharatpur, Nepal |
| title_fullStr | Multi-drug resistant extended-spectrum beta-lactamase producing E. coli and Salmonella on raw vegetable salads served at hotels and restaurants in Bharatpur, Nepal |
| title_full_unstemmed | Multi-drug resistant extended-spectrum beta-lactamase producing E. coli and Salmonella on raw vegetable salads served at hotels and restaurants in Bharatpur, Nepal |
| title_short | Multi-drug resistant extended-spectrum beta-lactamase producing E. coli and Salmonella on raw vegetable salads served at hotels and restaurants in Bharatpur, Nepal |
| title_sort | multi drug resistant extended spectrum beta lactamase producing e coli and salmonella on raw vegetable salads served at hotels and restaurants in bharatpur nepal |
| topic | Salads E. coli O157: H7 Antibiograms Multi-drug resistant ESBL Hotels and restaurants |
| url | http://link.springer.com/article/10.1186/s13104-019-4557-9 |
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