Effects of Stocking Density on Fatty Acid Metabolism by Skeletal Muscle in Mice
Specific pathogen-free (SPF) grade laboratory animals are kept in specific cages for life. The limited space could affect the characterization of colonization and dynamic changes related to gut microorganisms, and affect adipokines, even further affecting the fat synthesis and muscle quality of anim...
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MDPI AG
2022-09-01
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| Online Access: | https://www.mdpi.com/2076-2615/12/19/2538 |
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| author | Qiuyan Chen Xiaohui Li Jiarun Cui Caiyun Xu Hongfei Wei Qian Zhao Hongli Yao Hailong You Dawei Zhang Huimei Yu |
| author_facet | Qiuyan Chen Xiaohui Li Jiarun Cui Caiyun Xu Hongfei Wei Qian Zhao Hongli Yao Hailong You Dawei Zhang Huimei Yu |
| author_sort | Qiuyan Chen |
| collection | DOAJ |
| description | Specific pathogen-free (SPF) grade laboratory animals are kept in specific cages for life. The limited space could affect the characterization of colonization and dynamic changes related to gut microorganisms, and affect adipokines, even further affecting the fat synthesis and muscle quality of animals. The objective of this study was to analyze the stocking density on the dynamic distribution of gut microbiota, fat synthesis and muscle quality of SPF grade Kunming mice. Three housing densities were accomplished by raising different mice per cage with the same floor size. Kunming mice were reared at low stocking density (LSD, three mice a group), medium stocking density (MSD, 5 mice a group), and high stocking density (HSD, 10 mice a group) for 12 weeks. The results demonstrated that the stocking density affected intestinal microbial flora composition. We found that compared with the MSD group, the abundance of Lactobacillus in the LSD group and the HSD group decreased, but the abundance of unclassified_Porphyromonadaceae increased. Moreover, fat synthesis and muscle quality were linked to the intestinal microbial flora and stocking density. Compared with the LSD group and the HSD group, the MSD group had a more balanced gut flora, higher fat synthesis and higher muscle quality. Overall, this study demonstrated that stocking density could affect gut microbiota composition, and reasonable stocking density could improve fat synthesis and muscle quality. Our study will provide theoretical support for the suitable stocking density of laboratory animals. |
| first_indexed | 2024-03-09T22:09:29Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T22:09:29Z |
| publishDate | 2022-09-01 |
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| series | Animals |
| spelling | doaj.art-7cd3ff0fd28d48cb98f962728b2ec2312023-11-23T19:35:22ZengMDPI AGAnimals2076-26152022-09-011219253810.3390/ani12192538Effects of Stocking Density on Fatty Acid Metabolism by Skeletal Muscle in MiceQiuyan Chen0Xiaohui Li1Jiarun Cui2Caiyun Xu3Hongfei Wei4Qian Zhao5Hongli Yao6Hailong You7Dawei Zhang8Huimei Yu9Center of Animal Experiment, College of Basic Medical Sciences, Jilin University, Changchun 130021, ChinaCenter of Animal Experiment, College of Basic Medical Sciences, Jilin University, Changchun 130021, ChinaCenter of Animal Experiment, College of Basic Medical Sciences, Jilin University, Changchun 130021, ChinaCenter of Animal Experiment, College of Basic Medical Sciences, Jilin University, Changchun 130021, ChinaCenter of Animal Experiment, College of Basic Medical Sciences, Jilin University, Changchun 130021, ChinaKey Laboratory of Pathobiology, Ministry of Education, Department of Pathophysiology, College of Basic Medical Sciences, Jilin University, Changchun 130021, ChinaKey Laboratory of Pathobiology, Ministry of Education, Department of Pathophysiology, College of Basic Medical Sciences, Jilin University, Changchun 130021, ChinaKey Laboratory of Pathobiology, Ministry of Education, Department of Pathophysiology, College of Basic Medical Sciences, Jilin University, Changchun 130021, ChinaCenter of Animal Experiment, College of Basic Medical Sciences, Jilin University, Changchun 130021, ChinaCenter of Animal Experiment, College of Basic Medical Sciences, Jilin University, Changchun 130021, ChinaSpecific pathogen-free (SPF) grade laboratory animals are kept in specific cages for life. The limited space could affect the characterization of colonization and dynamic changes related to gut microorganisms, and affect adipokines, even further affecting the fat synthesis and muscle quality of animals. The objective of this study was to analyze the stocking density on the dynamic distribution of gut microbiota, fat synthesis and muscle quality of SPF grade Kunming mice. Three housing densities were accomplished by raising different mice per cage with the same floor size. Kunming mice were reared at low stocking density (LSD, three mice a group), medium stocking density (MSD, 5 mice a group), and high stocking density (HSD, 10 mice a group) for 12 weeks. The results demonstrated that the stocking density affected intestinal microbial flora composition. We found that compared with the MSD group, the abundance of Lactobacillus in the LSD group and the HSD group decreased, but the abundance of unclassified_Porphyromonadaceae increased. Moreover, fat synthesis and muscle quality were linked to the intestinal microbial flora and stocking density. Compared with the LSD group and the HSD group, the MSD group had a more balanced gut flora, higher fat synthesis and higher muscle quality. Overall, this study demonstrated that stocking density could affect gut microbiota composition, and reasonable stocking density could improve fat synthesis and muscle quality. Our study will provide theoretical support for the suitable stocking density of laboratory animals.https://www.mdpi.com/2076-2615/12/19/2538SPF Kunming miceintestinal florafatty acidsstocking densitymuscle quality |
| spellingShingle | Qiuyan Chen Xiaohui Li Jiarun Cui Caiyun Xu Hongfei Wei Qian Zhao Hongli Yao Hailong You Dawei Zhang Huimei Yu Effects of Stocking Density on Fatty Acid Metabolism by Skeletal Muscle in Mice Animals SPF Kunming mice intestinal flora fatty acids stocking density muscle quality |
| title | Effects of Stocking Density on Fatty Acid Metabolism by Skeletal Muscle in Mice |
| title_full | Effects of Stocking Density on Fatty Acid Metabolism by Skeletal Muscle in Mice |
| title_fullStr | Effects of Stocking Density on Fatty Acid Metabolism by Skeletal Muscle in Mice |
| title_full_unstemmed | Effects of Stocking Density on Fatty Acid Metabolism by Skeletal Muscle in Mice |
| title_short | Effects of Stocking Density on Fatty Acid Metabolism by Skeletal Muscle in Mice |
| title_sort | effects of stocking density on fatty acid metabolism by skeletal muscle in mice |
| topic | SPF Kunming mice intestinal flora fatty acids stocking density muscle quality |
| url | https://www.mdpi.com/2076-2615/12/19/2538 |
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