Hair follicle dermal condensation forms via Fgf20 primed cell cycle exit, cell motility, and aggregation
Mesenchymal condensation is a critical step in organogenesis, yet the underlying molecular and cellular mechanisms remain poorly understood. The hair follicle dermal condensate is the precursor to the permanent mesenchymal unit of the hair follicle, the dermal papilla, which regulates hair cycling t...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2018-07-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/36468 |
| _version_ | 1811235924817739776 |
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| author | Leah C Biggs Otto JM Mäkelä Satu-Marja Myllymäki Rishi Das Roy Katja Närhi Johanna Pispa Tuija Mustonen Marja L Mikkola |
| author_facet | Leah C Biggs Otto JM Mäkelä Satu-Marja Myllymäki Rishi Das Roy Katja Närhi Johanna Pispa Tuija Mustonen Marja L Mikkola |
| author_sort | Leah C Biggs |
| collection | DOAJ |
| description | Mesenchymal condensation is a critical step in organogenesis, yet the underlying molecular and cellular mechanisms remain poorly understood. The hair follicle dermal condensate is the precursor to the permanent mesenchymal unit of the hair follicle, the dermal papilla, which regulates hair cycling throughout life and bears hair inductive potential. Dermal condensate morphogenesis depends on epithelial Fibroblast Growth Factor 20 (Fgf20). Here, we combine mouse models with 3D and 4D microscopy to demonstrate that dermal condensates form de novo and via directional migration. We identify cell cycle exit and cell shape changes as early hallmarks of dermal condensate morphogenesis and find that Fgf20 primes these cellular behaviors and enhances cell motility and condensation. RNAseq profiling of immediate Fgf20 targets revealed induction of a subset of dermal condensate marker genes. Collectively, these data indicate that dermal condensation occurs via directed cell movement and that Fgf20 orchestrates the early cellular and molecular events. |
| first_indexed | 2024-04-12T12:00:27Z |
| format | Article |
| id | doaj.art-7cd730ec9c4c493fadcff881c9fac35c |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T12:00:27Z |
| publishDate | 2018-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-7cd730ec9c4c493fadcff881c9fac35c2022-12-22T03:33:52ZengeLife Sciences Publications LtdeLife2050-084X2018-07-01710.7554/eLife.36468Hair follicle dermal condensation forms via Fgf20 primed cell cycle exit, cell motility, and aggregationLeah C Biggs0https://orcid.org/0000-0002-4990-8664Otto JM Mäkelä1https://orcid.org/0000-0001-6852-9814Satu-Marja Myllymäki2Rishi Das Roy3https://orcid.org/0000-0002-3276-7279Katja Närhi4Johanna Pispa5Tuija Mustonen6https://orcid.org/0000-0002-2429-5064Marja L Mikkola7https://orcid.org/0000-0002-9890-3835Developmental Biology Program, Institute of Biotechnology, University of Helsinki, Helsinki, FinlandDevelopmental Biology Program, Institute of Biotechnology, University of Helsinki, Helsinki, FinlandDevelopmental Biology Program, Institute of Biotechnology, University of Helsinki, Helsinki, FinlandDevelopmental Biology Program, Institute of Biotechnology, University of Helsinki, Helsinki, FinlandDevelopmental Biology Program, Institute of Biotechnology, University of Helsinki, Helsinki, FinlandDevelopmental Biology Program, Institute of Biotechnology, University of Helsinki, Helsinki, FinlandDevelopmental Biology Program, Institute of Biotechnology, University of Helsinki, Helsinki, FinlandDevelopmental Biology Program, Institute of Biotechnology, University of Helsinki, Helsinki, FinlandMesenchymal condensation is a critical step in organogenesis, yet the underlying molecular and cellular mechanisms remain poorly understood. The hair follicle dermal condensate is the precursor to the permanent mesenchymal unit of the hair follicle, the dermal papilla, which regulates hair cycling throughout life and bears hair inductive potential. Dermal condensate morphogenesis depends on epithelial Fibroblast Growth Factor 20 (Fgf20). Here, we combine mouse models with 3D and 4D microscopy to demonstrate that dermal condensates form de novo and via directional migration. We identify cell cycle exit and cell shape changes as early hallmarks of dermal condensate morphogenesis and find that Fgf20 primes these cellular behaviors and enhances cell motility and condensation. RNAseq profiling of immediate Fgf20 targets revealed induction of a subset of dermal condensate marker genes. Collectively, these data indicate that dermal condensation occurs via directed cell movement and that Fgf20 orchestrates the early cellular and molecular events.https://elifesciences.org/articles/36468hair follicledermal condensateFgf20RNAseqlive imaging |
| spellingShingle | Leah C Biggs Otto JM Mäkelä Satu-Marja Myllymäki Rishi Das Roy Katja Närhi Johanna Pispa Tuija Mustonen Marja L Mikkola Hair follicle dermal condensation forms via Fgf20 primed cell cycle exit, cell motility, and aggregation eLife hair follicle dermal condensate Fgf20 RNAseq live imaging |
| title | Hair follicle dermal condensation forms via Fgf20 primed cell cycle exit, cell motility, and aggregation |
| title_full | Hair follicle dermal condensation forms via Fgf20 primed cell cycle exit, cell motility, and aggregation |
| title_fullStr | Hair follicle dermal condensation forms via Fgf20 primed cell cycle exit, cell motility, and aggregation |
| title_full_unstemmed | Hair follicle dermal condensation forms via Fgf20 primed cell cycle exit, cell motility, and aggregation |
| title_short | Hair follicle dermal condensation forms via Fgf20 primed cell cycle exit, cell motility, and aggregation |
| title_sort | hair follicle dermal condensation forms via fgf20 primed cell cycle exit cell motility and aggregation |
| topic | hair follicle dermal condensate Fgf20 RNAseq live imaging |
| url | https://elifesciences.org/articles/36468 |
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