Rapid Lipid Content Screening in <em>Neochloris oleoabundans</em> Utilizing Carbon-Based Dielectrophoresis
In this study, we carried out a heterogeneous cytoplasmic lipid content screening of Neochloris oleoabundans microalgae by dielectrophoresis (DEP), using castellated glassy carbon microelectrodes in a PDMS microchannel. For this purpose, microalgae were cultured in nitrogen-replete (N+) and nitrogen...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/12/9/1023 |
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| author | Cynthia M. Galicia-Medina Matías Vázquez-Piñón Gibran S. Alemán-Nava Roberto C. Gallo-Villanueva Sergio O. Martínez-Chapa Marc J. Madou Sergio Camacho-León Jonathan S. García-Pérez Diego A. Esquivel-Hernández Roberto Parra-Saldívar Víctor H. Pérez-González |
| author_facet | Cynthia M. Galicia-Medina Matías Vázquez-Piñón Gibran S. Alemán-Nava Roberto C. Gallo-Villanueva Sergio O. Martínez-Chapa Marc J. Madou Sergio Camacho-León Jonathan S. García-Pérez Diego A. Esquivel-Hernández Roberto Parra-Saldívar Víctor H. Pérez-González |
| author_sort | Cynthia M. Galicia-Medina |
| collection | DOAJ |
| description | In this study, we carried out a heterogeneous cytoplasmic lipid content screening of Neochloris oleoabundans microalgae by dielectrophoresis (DEP), using castellated glassy carbon microelectrodes in a PDMS microchannel. For this purpose, microalgae were cultured in nitrogen-replete (N+) and nitrogen-deplete (N−) suspensions to promote low and high cytoplasmic lipid production in cells, respectively. Experiments were carried out over a wide frequency window (100 kHz–30 MHz) at a fixed amplitude of 7 V<sub>PP</sub>. The results showed a statistically significant difference between the dielectrophoretic behavior of N+ and N− cells at low frequencies (100–800 kHz), whereas a weak response was observed for mid- and high frequencies (1–30 MHz). Additionally, a finite element analysis using a 3D model was conducted to determine the dielectrophoretic trapping zones across the electrode gaps. These results suggest that low-cost glassy carbon is a reliable material for microalgae classification—between low and high cytoplasmic lipid content—through DEP, providing a fast and straightforward mechanism. |
| first_indexed | 2024-03-10T07:26:11Z |
| format | Article |
| id | doaj.art-7cd8326619fc48feb08b37eefcf5ed09 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T07:26:11Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-7cd8326619fc48feb08b37eefcf5ed092023-11-22T14:15:26ZengMDPI AGMicromachines2072-666X2021-08-01129102310.3390/mi12091023Rapid Lipid Content Screening in <em>Neochloris oleoabundans</em> Utilizing Carbon-Based DielectrophoresisCynthia M. Galicia-Medina0Matías Vázquez-Piñón1Gibran S. Alemán-Nava2Roberto C. Gallo-Villanueva3Sergio O. Martínez-Chapa4Marc J. Madou5Sergio Camacho-León6Jonathan S. García-Pérez7Diego A. Esquivel-Hernández8Roberto Parra-Saldívar9Víctor H. Pérez-González10Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Ave. Eugenio Garza Sada 2501, Monterrey 64849, MexicoTecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Ave. Eugenio Garza Sada 2501, Monterrey 64849, MexicoTecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Ave. Eugenio Garza Sada 2501, Monterrey 64849, MexicoTecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Ave. Eugenio Garza Sada 2501, Monterrey 64849, MexicoTecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Ave. Eugenio Garza Sada 2501, Monterrey 64849, MexicoDepartment of Mechanical and Aerospace Engineering, University of California, 4200 Engineering Gateway, Irvine, CA 92697, USATecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Ave. Eugenio Garza Sada 2501, Monterrey 64849, MexicoTecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Ave. Eugenio Garza Sada 2501, Monterrey 64849, MexicoDepartamento de Biología Celular, Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad de México 04510, MexicoTecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Ave. Eugenio Garza Sada 2501, Monterrey 64849, MexicoTecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Ave. Eugenio Garza Sada 2501, Monterrey 64849, MexicoIn this study, we carried out a heterogeneous cytoplasmic lipid content screening of Neochloris oleoabundans microalgae by dielectrophoresis (DEP), using castellated glassy carbon microelectrodes in a PDMS microchannel. For this purpose, microalgae were cultured in nitrogen-replete (N+) and nitrogen-deplete (N−) suspensions to promote low and high cytoplasmic lipid production in cells, respectively. Experiments were carried out over a wide frequency window (100 kHz–30 MHz) at a fixed amplitude of 7 V<sub>PP</sub>. The results showed a statistically significant difference between the dielectrophoretic behavior of N+ and N− cells at low frequencies (100–800 kHz), whereas a weak response was observed for mid- and high frequencies (1–30 MHz). Additionally, a finite element analysis using a 3D model was conducted to determine the dielectrophoretic trapping zones across the electrode gaps. These results suggest that low-cost glassy carbon is a reliable material for microalgae classification—between low and high cytoplasmic lipid content—through DEP, providing a fast and straightforward mechanism.https://www.mdpi.com/2072-666X/12/9/1023dielectrophoresismicroalgaebiofuelmicrofluidicscarbon-microelectromechanical systems |
| spellingShingle | Cynthia M. Galicia-Medina Matías Vázquez-Piñón Gibran S. Alemán-Nava Roberto C. Gallo-Villanueva Sergio O. Martínez-Chapa Marc J. Madou Sergio Camacho-León Jonathan S. García-Pérez Diego A. Esquivel-Hernández Roberto Parra-Saldívar Víctor H. Pérez-González Rapid Lipid Content Screening in <em>Neochloris oleoabundans</em> Utilizing Carbon-Based Dielectrophoresis Micromachines dielectrophoresis microalgae biofuel microfluidics carbon-microelectromechanical systems |
| title | Rapid Lipid Content Screening in <em>Neochloris oleoabundans</em> Utilizing Carbon-Based Dielectrophoresis |
| title_full | Rapid Lipid Content Screening in <em>Neochloris oleoabundans</em> Utilizing Carbon-Based Dielectrophoresis |
| title_fullStr | Rapid Lipid Content Screening in <em>Neochloris oleoabundans</em> Utilizing Carbon-Based Dielectrophoresis |
| title_full_unstemmed | Rapid Lipid Content Screening in <em>Neochloris oleoabundans</em> Utilizing Carbon-Based Dielectrophoresis |
| title_short | Rapid Lipid Content Screening in <em>Neochloris oleoabundans</em> Utilizing Carbon-Based Dielectrophoresis |
| title_sort | rapid lipid content screening in em neochloris oleoabundans em utilizing carbon based dielectrophoresis |
| topic | dielectrophoresis microalgae biofuel microfluidics carbon-microelectromechanical systems |
| url | https://www.mdpi.com/2072-666X/12/9/1023 |
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