Antimicrobial resistance patterns of commensal Escherichia coli isolated from feces of non-diarrheic dogs in Grenada, West Indies
Background and Aim: There is currently no published information on the prevalence and antimicrobial susceptibility patterns of commensal Escherichia coli in dogs of Grenada origin. Monitoring antimicrobial resistance helps in the empirical selection of antibiotics. This study determined the occurren...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Veterinary World
2019-12-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.12/December-2019/25.pdf |
| _version_ | 1819137081526452224 |
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| author | Victor A. Amadi Harry Hariharan Ozioma A. Amadi Vanessa Matthew-Belmar Roxanne Nicholas-Thomas Marta Lanza Perea Kenrith Carter Eugene Rennie Keith Kalasi Andy Alhassan Richard M. Kabuusu Grant Ugochukwu Alozie Paul J. Fields Rhonda Pinckney Ravindra Sharma |
| author_facet | Victor A. Amadi Harry Hariharan Ozioma A. Amadi Vanessa Matthew-Belmar Roxanne Nicholas-Thomas Marta Lanza Perea Kenrith Carter Eugene Rennie Keith Kalasi Andy Alhassan Richard M. Kabuusu Grant Ugochukwu Alozie Paul J. Fields Rhonda Pinckney Ravindra Sharma |
| author_sort | Victor A. Amadi |
| collection | DOAJ |
| description | Background and Aim: There is currently no published information on the prevalence and antimicrobial susceptibility patterns of commensal Escherichia coli in dogs of Grenada origin. Monitoring antimicrobial resistance helps in the empirical selection of antibiotics. This study determined the occurrence of E. coli including the O157:H7 serotype in feces of non-diarrheic dogs of Grenada origin and the antibiotic resistance pattern of the E. coli isolates.
Materials and Methods: Fecal samples from 142 of the 144 (98.6%) dogs were culture positive for E. coli. Selection of up to three colonies from each of the 142 E. coli-positive samples yielded a total of 402 E. coli isolates, which were analyzed for the presence of non-sorbitol fermenting colonies, and O157-agglutination.
Results: Of the 402 E. coli isolates, 30 (7.5%) were non-sorbitol fermenters. However, none of the 402 isolates gave a positive reaction (O157:H7) to the E. coli O157:H7 latex kit. Antimicrobial susceptibility tests against 12 antibiotics revealed low resistance rates to all the tested antibiotics except for tetracycline (Te) (23.4%), cephalothin (CF) (13.2%), and ampicillin (AM) (7.7%). Thirty-nine out of the 402 (9.7%), E. coli isolates were resistant to two or more antibiotics of different classes.
Conclusion: This is the first report of isolation and antimicrobial susceptibilities of commensal E. coli from non-diarrheic dogs in Grenada. Some of the isolates (39/402 isolates, 9.7%) were resistant to multiple antibiotics. This study showed that presently, dogs in Grenada should not be considered a reservoir for the E. coli O157:H7 serotype and for multiple antibiotic-resistant E. coli strains. Among the 402 E. coli isolates, the resistance rate to drugs other than Te, CF, and AM was very low. |
| first_indexed | 2024-12-22T10:45:12Z |
| format | Article |
| id | doaj.art-7cd87aa73f1d4c7eaca2badbc597cba7 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-22T10:45:12Z |
| publishDate | 2019-12-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-7cd87aa73f1d4c7eaca2badbc597cba72022-12-21T18:28:57ZengVeterinary WorldVeterinary World0972-89882231-09162019-12-0112122070207510.14202/vetworld.2019.2070-2075Antimicrobial resistance patterns of commensal Escherichia coli isolated from feces of non-diarrheic dogs in Grenada, West IndiesVictor A. Amadi0Harry Hariharan1Ozioma A. Amadi2Vanessa Matthew-Belmar3Roxanne Nicholas-Thomas4Marta Lanza Perea5Kenrith Carter6Eugene Rennie7Keith Kalasi8Andy Alhassan9Richard M. Kabuusu10Grant Ugochukwu Alozie11Paul J. Fields12Rhonda Pinckney13Ravindra Sharma14Department of Pathobiology, School of Veterinary Medicine, St. George's University, St. George's, Grenada, West Indies.Department of Pathobiology, School of Veterinary Medicine, St. George's University, St. George's, Grenada, West Indies.Department of Public Health and Preventative Medicine, School of Medicine, St. George's University, Grenada, West Indies.Department of Pathobiology, School of Veterinary Medicine, St. George's University, St. George's, Grenada, West Indies.Department of Pathobiology, School of Veterinary Medicine, St. George's University, St. George's, Grenada, West Indies.Department of Small Animal Medicine and Surgery, School of Veterinary Medicine, St. George's University, St. George's, Grenada, West Indies.Department of Pathobiology, School of Veterinary Medicine, St. George's University, St. George's, Grenada, West Indies.Department of Anatomy, Physiology and Pharmacology, School of Veterinary Medicine, St. George's University, St. George's, Grenada, West Indies.Department of Small Animal Clinic, School of Veterinary Medicine, St. George's University, St. George's, Grenada, West Indies.Department of Pathobiology, School of Veterinary Medicine, St. George's University, St. George's, Grenada, West Indies.Department of Pathobiology, School of Veterinary Medicine, St. George's University, St. George's, Grenada, West Indies.Department of Neuroscience, Physiology and Behavioral Science, School of Medicine, St. George's University, St. George's, Grenada, West Indies.Office of Research, School of Graduate Studies, St. George's University, St. George's, Grenada, West Indies.Department of Pathobiology, School of Veterinary Medicine, St. George's University, St. George's, Grenada, West Indies.Department of Pathobiology, School of Veterinary Medicine, St. George's University, St. George's, Grenada, West Indies.Background and Aim: There is currently no published information on the prevalence and antimicrobial susceptibility patterns of commensal Escherichia coli in dogs of Grenada origin. Monitoring antimicrobial resistance helps in the empirical selection of antibiotics. This study determined the occurrence of E. coli including the O157:H7 serotype in feces of non-diarrheic dogs of Grenada origin and the antibiotic resistance pattern of the E. coli isolates. Materials and Methods: Fecal samples from 142 of the 144 (98.6%) dogs were culture positive for E. coli. Selection of up to three colonies from each of the 142 E. coli-positive samples yielded a total of 402 E. coli isolates, which were analyzed for the presence of non-sorbitol fermenting colonies, and O157-agglutination. Results: Of the 402 E. coli isolates, 30 (7.5%) were non-sorbitol fermenters. However, none of the 402 isolates gave a positive reaction (O157:H7) to the E. coli O157:H7 latex kit. Antimicrobial susceptibility tests against 12 antibiotics revealed low resistance rates to all the tested antibiotics except for tetracycline (Te) (23.4%), cephalothin (CF) (13.2%), and ampicillin (AM) (7.7%). Thirty-nine out of the 402 (9.7%), E. coli isolates were resistant to two or more antibiotics of different classes. Conclusion: This is the first report of isolation and antimicrobial susceptibilities of commensal E. coli from non-diarrheic dogs in Grenada. Some of the isolates (39/402 isolates, 9.7%) were resistant to multiple antibiotics. This study showed that presently, dogs in Grenada should not be considered a reservoir for the E. coli O157:H7 serotype and for multiple antibiotic-resistant E. coli strains. Among the 402 E. coli isolates, the resistance rate to drugs other than Te, CF, and AM was very low.http://www.veterinaryworld.org/Vol.12/December-2019/25.pdfantimicrobial resistancecommensal escherichia colidogsgrenada |
| spellingShingle | Victor A. Amadi Harry Hariharan Ozioma A. Amadi Vanessa Matthew-Belmar Roxanne Nicholas-Thomas Marta Lanza Perea Kenrith Carter Eugene Rennie Keith Kalasi Andy Alhassan Richard M. Kabuusu Grant Ugochukwu Alozie Paul J. Fields Rhonda Pinckney Ravindra Sharma Antimicrobial resistance patterns of commensal Escherichia coli isolated from feces of non-diarrheic dogs in Grenada, West Indies Veterinary World antimicrobial resistance commensal escherichia coli dogs grenada |
| title | Antimicrobial resistance patterns of commensal Escherichia coli isolated from feces of non-diarrheic dogs in Grenada, West Indies |
| title_full | Antimicrobial resistance patterns of commensal Escherichia coli isolated from feces of non-diarrheic dogs in Grenada, West Indies |
| title_fullStr | Antimicrobial resistance patterns of commensal Escherichia coli isolated from feces of non-diarrheic dogs in Grenada, West Indies |
| title_full_unstemmed | Antimicrobial resistance patterns of commensal Escherichia coli isolated from feces of non-diarrheic dogs in Grenada, West Indies |
| title_short | Antimicrobial resistance patterns of commensal Escherichia coli isolated from feces of non-diarrheic dogs in Grenada, West Indies |
| title_sort | antimicrobial resistance patterns of commensal escherichia coli isolated from feces of non diarrheic dogs in grenada west indies |
| topic | antimicrobial resistance commensal escherichia coli dogs grenada |
| url | http://www.veterinaryworld.org/Vol.12/December-2019/25.pdf |
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