The Antioxidant Guaiacol Exerts Fungicidal Activity Against Fungal Growth and Deoxynivalenol Production in Fusarium graminearum
The main component of creosote obtained from dry wood distillation—guaiacol—is a natural antioxidant that has been widely used in pharmaceutical and food preservation applications. However, the antifungal mechanism of guaiacol against phytopathogens remains unclear. In this study, we found that guai...
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.762844/full |
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| author | Tao Gao Tao Gao Tao Gao Tao Gao Yao Zhang Jianrong Shi Jianrong Shi Jianrong Shi Jianrong Shi Jianrong Shi Sherif Ramzy Mohamed Jianhong Xu Jianhong Xu Jianhong Xu Jianhong Xu Jianhong Xu Xin Liu Xin Liu Xin Liu Xin Liu Xin Liu |
| author_facet | Tao Gao Tao Gao Tao Gao Tao Gao Yao Zhang Jianrong Shi Jianrong Shi Jianrong Shi Jianrong Shi Jianrong Shi Sherif Ramzy Mohamed Jianhong Xu Jianhong Xu Jianhong Xu Jianhong Xu Jianhong Xu Xin Liu Xin Liu Xin Liu Xin Liu Xin Liu |
| author_sort | Tao Gao |
| collection | DOAJ |
| description | The main component of creosote obtained from dry wood distillation—guaiacol—is a natural antioxidant that has been widely used in pharmaceutical and food preservation applications. However, the antifungal mechanism of guaiacol against phytopathogens remains unclear. In this study, we found that guaiacol exerts inhibitory effects against mycelial growth, conidial formation and germination, and deoxynivalenol (DON) biosynthesis in Fusarium graminearum in a dose-dependent manner. The median effective concentration (EC50) value of guaiacol for the standard F. graminearum strain PH-1 was 1.838 mM. Guaiacol strongly inhibited conidial production and germination. The antifungal effects of guaiacol may be attributed to its capability to cause damage to the cell membrane by disrupting Ca2+ transport channels. In addition, the decreased malondialdehyde (MDA) levels and catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) activity by guaiacol treatment indicate that guaiacol displays activity against DON production by modulating the oxidative response in F. graminearum. Taken together, this study revealed the potentials of antioxidant in inhibiting mycotoxins in F. graminearum. |
| first_indexed | 2024-12-19T03:08:31Z |
| format | Article |
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| language | English |
| last_indexed | 2024-12-19T03:08:31Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-7cd99315bc7b49a98469342ba3766aea2022-12-21T20:38:04ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-11-011210.3389/fmicb.2021.762844762844The Antioxidant Guaiacol Exerts Fungicidal Activity Against Fungal Growth and Deoxynivalenol Production in Fusarium graminearumTao Gao0Tao Gao1Tao Gao2Tao Gao3Yao Zhang4Jianrong Shi5Jianrong Shi6Jianrong Shi7Jianrong Shi8Jianrong Shi9Sherif Ramzy Mohamed10Jianhong Xu11Jianhong Xu12Jianhong Xu13Jianhong Xu14Jianhong Xu15Xin Liu16Xin Liu17Xin Liu18Xin Liu19Xin Liu20Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory for Control Technology and Standard for Agro-Product Safety and Quality, Ministry of Agriculture and Rural Affairs, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory for Agro-Product Safety Risk Evaluation (Nanjing), Ministry of Agriculture and Rural Affairs, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaCollaborative Innovation Center for Modern Grain Circulation and Safety, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaSchool of Food Science And Engineering, Jiangsu Ocean University, Lianyungang, ChinaJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory for Control Technology and Standard for Agro-Product Safety and Quality, Ministry of Agriculture and Rural Affairs, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory for Agro-Product Safety Risk Evaluation (Nanjing), Ministry of Agriculture and Rural Affairs, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaCollaborative Innovation Center for Modern Grain Circulation and Safety, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang, ChinaDepartment of Food Toxicology and Contaminant, National Research Centre of Egypt, Giza, EgyptJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory for Control Technology and Standard for Agro-Product Safety and Quality, Ministry of Agriculture and Rural Affairs, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory for Agro-Product Safety Risk Evaluation (Nanjing), Ministry of Agriculture and Rural Affairs, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaCollaborative Innovation Center for Modern Grain Circulation and Safety, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang, ChinaJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory for Control Technology and Standard for Agro-Product Safety and Quality, Ministry of Agriculture and Rural Affairs, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaKey Laboratory for Agro-Product Safety Risk Evaluation (Nanjing), Ministry of Agriculture and Rural Affairs, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaCollaborative Innovation Center for Modern Grain Circulation and Safety, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang, ChinaThe main component of creosote obtained from dry wood distillation—guaiacol—is a natural antioxidant that has been widely used in pharmaceutical and food preservation applications. However, the antifungal mechanism of guaiacol against phytopathogens remains unclear. In this study, we found that guaiacol exerts inhibitory effects against mycelial growth, conidial formation and germination, and deoxynivalenol (DON) biosynthesis in Fusarium graminearum in a dose-dependent manner. The median effective concentration (EC50) value of guaiacol for the standard F. graminearum strain PH-1 was 1.838 mM. Guaiacol strongly inhibited conidial production and germination. The antifungal effects of guaiacol may be attributed to its capability to cause damage to the cell membrane by disrupting Ca2+ transport channels. In addition, the decreased malondialdehyde (MDA) levels and catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) activity by guaiacol treatment indicate that guaiacol displays activity against DON production by modulating the oxidative response in F. graminearum. Taken together, this study revealed the potentials of antioxidant in inhibiting mycotoxins in F. graminearum.https://www.frontiersin.org/articles/10.3389/fmicb.2021.762844/fullFusarium graminearumguaiacolintracellular Ca2+deoxynivalenolantioxidant |
| spellingShingle | Tao Gao Tao Gao Tao Gao Tao Gao Yao Zhang Jianrong Shi Jianrong Shi Jianrong Shi Jianrong Shi Jianrong Shi Sherif Ramzy Mohamed Jianhong Xu Jianhong Xu Jianhong Xu Jianhong Xu Jianhong Xu Xin Liu Xin Liu Xin Liu Xin Liu Xin Liu The Antioxidant Guaiacol Exerts Fungicidal Activity Against Fungal Growth and Deoxynivalenol Production in Fusarium graminearum Frontiers in Microbiology Fusarium graminearum guaiacol intracellular Ca2+ deoxynivalenol antioxidant |
| title | The Antioxidant Guaiacol Exerts Fungicidal Activity Against Fungal Growth and Deoxynivalenol Production in Fusarium graminearum |
| title_full | The Antioxidant Guaiacol Exerts Fungicidal Activity Against Fungal Growth and Deoxynivalenol Production in Fusarium graminearum |
| title_fullStr | The Antioxidant Guaiacol Exerts Fungicidal Activity Against Fungal Growth and Deoxynivalenol Production in Fusarium graminearum |
| title_full_unstemmed | The Antioxidant Guaiacol Exerts Fungicidal Activity Against Fungal Growth and Deoxynivalenol Production in Fusarium graminearum |
| title_short | The Antioxidant Guaiacol Exerts Fungicidal Activity Against Fungal Growth and Deoxynivalenol Production in Fusarium graminearum |
| title_sort | antioxidant guaiacol exerts fungicidal activity against fungal growth and deoxynivalenol production in fusarium graminearum |
| topic | Fusarium graminearum guaiacol intracellular Ca2+ deoxynivalenol antioxidant |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.762844/full |
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