Systematic Characterization of GATA Transcription Factors in <i>Liriodendron chinense</i> and Functional Validation in Abiotic Stresses
The <i>Liriodendron chinense</i> in the <i>Magnoliaceae</i> family is an endangered tree species useful for its socio-economic and ecological benefits. Abiotic stresses (cold, heat, and drought stress), among other factors, affect its growth, development, and distribution. Ho...
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MDPI AG
2023-06-01
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| Series: | Plants |
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| author | Delight Hwarari Yasmina Radani Yuanlin Guan Jinhui Chen Yang Liming |
| author_facet | Delight Hwarari Yasmina Radani Yuanlin Guan Jinhui Chen Yang Liming |
| author_sort | Delight Hwarari |
| collection | DOAJ |
| description | The <i>Liriodendron chinense</i> in the <i>Magnoliaceae</i> family is an endangered tree species useful for its socio-economic and ecological benefits. Abiotic stresses (cold, heat, and drought stress), among other factors, affect its growth, development, and distribution. However, GATA transcription factors (TFs) respond to various abiotic stresses and play a significant role in plant acclimatization to abiotic stresses. To determine the function of GATA TFs in <i>L. chinense</i>, we investigated the GATA genes in the genome of <i>L. chinense</i>. In this study, a total of 18 GATA genes were identified, which were randomly distributed on 12 of the total 17 chromosomes. These GATA genes clustered together in four separate groups based on their phylogenetic relationships, gene structures, and domain conservation arrangements. Detailed interspecies phylogenetic analyses of the GATA gene family demonstrated a conservation of the GATAs and a probable diversification that prompted gene diversification in plant species. In addition, the LcGATA gene family was shown to be evolutionarily closer to that of <i>O. sativa</i>, giving an insight into the possible LcGATA gene functions. Investigations of LcGATA gene duplication showed four gene duplicate pairs by the segmental duplication event, and these genes were a result of strong purified selection. Analysis of the <i>cis</i>-regulatory elements demonstrated a significant representation of the abiotic stress elements in the promoter regions of the LcGATA genes. Additional gene expressions through transcriptome and qPCR analyses revealed a significant upregulation of LcGATA17, and LcGATA18 in various stresses, including heat, cold, and drought stress in all time points analyzed. We concluded that the LcGATA genes play a pivotal role in regulating abiotic stress in <i>L. chinense</i>. In summary, our results provide new insights into understanding of the LcGATA gene family and their regulatory functions during abiotic stresses. |
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| spelling | doaj.art-7cdb499b725c4b539e7ce11211f832562023-11-18T12:10:53ZengMDPI AGPlants2223-77472023-06-011212234910.3390/plants12122349Systematic Characterization of GATA Transcription Factors in <i>Liriodendron chinense</i> and Functional Validation in Abiotic StressesDelight Hwarari0Yasmina Radani1Yuanlin Guan2Jinhui Chen3Yang Liming4State Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaThe <i>Liriodendron chinense</i> in the <i>Magnoliaceae</i> family is an endangered tree species useful for its socio-economic and ecological benefits. Abiotic stresses (cold, heat, and drought stress), among other factors, affect its growth, development, and distribution. However, GATA transcription factors (TFs) respond to various abiotic stresses and play a significant role in plant acclimatization to abiotic stresses. To determine the function of GATA TFs in <i>L. chinense</i>, we investigated the GATA genes in the genome of <i>L. chinense</i>. In this study, a total of 18 GATA genes were identified, which were randomly distributed on 12 of the total 17 chromosomes. These GATA genes clustered together in four separate groups based on their phylogenetic relationships, gene structures, and domain conservation arrangements. Detailed interspecies phylogenetic analyses of the GATA gene family demonstrated a conservation of the GATAs and a probable diversification that prompted gene diversification in plant species. In addition, the LcGATA gene family was shown to be evolutionarily closer to that of <i>O. sativa</i>, giving an insight into the possible LcGATA gene functions. Investigations of LcGATA gene duplication showed four gene duplicate pairs by the segmental duplication event, and these genes were a result of strong purified selection. Analysis of the <i>cis</i>-regulatory elements demonstrated a significant representation of the abiotic stress elements in the promoter regions of the LcGATA genes. Additional gene expressions through transcriptome and qPCR analyses revealed a significant upregulation of LcGATA17, and LcGATA18 in various stresses, including heat, cold, and drought stress in all time points analyzed. We concluded that the LcGATA genes play a pivotal role in regulating abiotic stress in <i>L. chinense</i>. In summary, our results provide new insights into understanding of the LcGATA gene family and their regulatory functions during abiotic stresses.https://www.mdpi.com/2223-7747/12/12/2349<i>Liriodendron chinense</i>GATA transcription factorsexpression patternabiotic stress |
| spellingShingle | Delight Hwarari Yasmina Radani Yuanlin Guan Jinhui Chen Yang Liming Systematic Characterization of GATA Transcription Factors in <i>Liriodendron chinense</i> and Functional Validation in Abiotic Stresses Plants <i>Liriodendron chinense</i> GATA transcription factors expression pattern abiotic stress |
| title | Systematic Characterization of GATA Transcription Factors in <i>Liriodendron chinense</i> and Functional Validation in Abiotic Stresses |
| title_full | Systematic Characterization of GATA Transcription Factors in <i>Liriodendron chinense</i> and Functional Validation in Abiotic Stresses |
| title_fullStr | Systematic Characterization of GATA Transcription Factors in <i>Liriodendron chinense</i> and Functional Validation in Abiotic Stresses |
| title_full_unstemmed | Systematic Characterization of GATA Transcription Factors in <i>Liriodendron chinense</i> and Functional Validation in Abiotic Stresses |
| title_short | Systematic Characterization of GATA Transcription Factors in <i>Liriodendron chinense</i> and Functional Validation in Abiotic Stresses |
| title_sort | systematic characterization of gata transcription factors in i liriodendron chinense i and functional validation in abiotic stresses |
| topic | <i>Liriodendron chinense</i> GATA transcription factors expression pattern abiotic stress |
| url | https://www.mdpi.com/2223-7747/12/12/2349 |
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