Effects of dietary supplementation with Lactobacillus acidophilus on the performance, intestinal physical barrier function, and the expression of NOD-like receptors in weaned piglets
Lactobacillus supplementation is beneficial to the barrier function of the intestinal physical barrier in piglets. However, the mechanisms underlying this beneficial function remain largely unknown. Here, we investigated the effects of dietary supplementation with Lactobacillus acidophilus on the pe...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2018-12-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/6060.pdf |
| _version_ | 1827606085249269760 |
|---|---|
| author | Shiqiong Wang Haihua Li Chenhong Du Qian Liu Dongji Yang Longbin Chen Qi Zhu Zhixiang Wang |
| author_facet | Shiqiong Wang Haihua Li Chenhong Du Qian Liu Dongji Yang Longbin Chen Qi Zhu Zhixiang Wang |
| author_sort | Shiqiong Wang |
| collection | DOAJ |
| description | Lactobacillus supplementation is beneficial to the barrier function of the intestinal physical barrier in piglets. However, the mechanisms underlying this beneficial function remain largely unknown. Here, we investigated the effects of dietary supplementation with Lactobacillus acidophilus on the performance, intestinal physical barrier functioning, and NOD-like receptors (NLRs) expression in weaned piglets. Sixteen weaned piglets were randomly allocated to two groups. The control group received a corn-soybean basal diet, while the treatment group received the same diet adding 0.1% L. acidophilus, for 14 days. As a result, dietary L. acidophilus supplementation was found to increase the average daily gain (ADG) (P < 0.05), reduced serum diamine oxidase (DAO) activity (P < 0.05), increased the mRNA expression and protein abundance of occludin in the jejunum and ileum (P < 0.01), reduced the mRNA levels of NOD1 (P < 0.01), receptor interacting serine/threonine kinase 2 (RIPK2) (P < 0.05), nuclear factor κB (NF-κB) (P < 0.01), NLR family pyrin domain containing 3 (NLRP3) (P < 0.01), caspase-1 (P < 0.01), interleukin 1β (IL-1β) (P < 0.05) and IL-18 (P < 0.01) in the jejunum tissues of the weaned pigs. The expression of NLRP3 (P < 0.05), caspase-1 (P < 0.01), IL-1β (P < 0.05) and IL-18 (P < 0.05) was also reduced in the ileum tissues of the weaned pigs. These results showed that L. acidophilus supplementation improves the growth performance, enhances the intestinal physical barrier function, and inhibits the expression of NOD1 and NLRP3 signaling-pathway-related genes in jejunum and ileum tissues. They also suggest that L. acidophilus enhances the intestinal physical barrier functioning by inhibiting IL-1β and IL-18 pro-inflammatory cytokines via the NOD1/NLRP3 signaling pathway in weaned piglets. |
| first_indexed | 2024-03-09T06:32:04Z |
| format | Article |
| id | doaj.art-7cdd9e73a67e41169de71f5f4c17335c |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:32:04Z |
| publishDate | 2018-12-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-7cdd9e73a67e41169de71f5f4c17335c2023-12-03T11:03:59ZengPeerJ Inc.PeerJ2167-83592018-12-016e606010.7717/peerj.6060Effects of dietary supplementation with Lactobacillus acidophilus on the performance, intestinal physical barrier function, and the expression of NOD-like receptors in weaned pigletsShiqiong Wang0Haihua Li1Chenhong Du2Qian Liu3Dongji Yang4Longbin Chen5Qi Zhu6Zhixiang Wang7College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaCollege of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaTianjin Institute of Animal Husbandry and Veterinary Medicine, Tianjin Academy of Agricultural Sciences, Tianjin, ChinaTianjin Institute of Animal Husbandry and Veterinary Medicine, Tianjin Academy of Agricultural Sciences, Tianjin, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaLactobacillus supplementation is beneficial to the barrier function of the intestinal physical barrier in piglets. However, the mechanisms underlying this beneficial function remain largely unknown. Here, we investigated the effects of dietary supplementation with Lactobacillus acidophilus on the performance, intestinal physical barrier functioning, and NOD-like receptors (NLRs) expression in weaned piglets. Sixteen weaned piglets were randomly allocated to two groups. The control group received a corn-soybean basal diet, while the treatment group received the same diet adding 0.1% L. acidophilus, for 14 days. As a result, dietary L. acidophilus supplementation was found to increase the average daily gain (ADG) (P < 0.05), reduced serum diamine oxidase (DAO) activity (P < 0.05), increased the mRNA expression and protein abundance of occludin in the jejunum and ileum (P < 0.01), reduced the mRNA levels of NOD1 (P < 0.01), receptor interacting serine/threonine kinase 2 (RIPK2) (P < 0.05), nuclear factor κB (NF-κB) (P < 0.01), NLR family pyrin domain containing 3 (NLRP3) (P < 0.01), caspase-1 (P < 0.01), interleukin 1β (IL-1β) (P < 0.05) and IL-18 (P < 0.01) in the jejunum tissues of the weaned pigs. The expression of NLRP3 (P < 0.05), caspase-1 (P < 0.01), IL-1β (P < 0.05) and IL-18 (P < 0.05) was also reduced in the ileum tissues of the weaned pigs. These results showed that L. acidophilus supplementation improves the growth performance, enhances the intestinal physical barrier function, and inhibits the expression of NOD1 and NLRP3 signaling-pathway-related genes in jejunum and ileum tissues. They also suggest that L. acidophilus enhances the intestinal physical barrier functioning by inhibiting IL-1β and IL-18 pro-inflammatory cytokines via the NOD1/NLRP3 signaling pathway in weaned piglets.https://peerj.com/articles/6060.pdfLactobacillus acidophilusIntestinal physical barrierWeaned pigletNLRs |
| spellingShingle | Shiqiong Wang Haihua Li Chenhong Du Qian Liu Dongji Yang Longbin Chen Qi Zhu Zhixiang Wang Effects of dietary supplementation with Lactobacillus acidophilus on the performance, intestinal physical barrier function, and the expression of NOD-like receptors in weaned piglets PeerJ Lactobacillus acidophilus Intestinal physical barrier Weaned piglet NLRs |
| title | Effects of dietary supplementation with Lactobacillus acidophilus on the performance, intestinal physical barrier function, and the expression of NOD-like receptors in weaned piglets |
| title_full | Effects of dietary supplementation with Lactobacillus acidophilus on the performance, intestinal physical barrier function, and the expression of NOD-like receptors in weaned piglets |
| title_fullStr | Effects of dietary supplementation with Lactobacillus acidophilus on the performance, intestinal physical barrier function, and the expression of NOD-like receptors in weaned piglets |
| title_full_unstemmed | Effects of dietary supplementation with Lactobacillus acidophilus on the performance, intestinal physical barrier function, and the expression of NOD-like receptors in weaned piglets |
| title_short | Effects of dietary supplementation with Lactobacillus acidophilus on the performance, intestinal physical barrier function, and the expression of NOD-like receptors in weaned piglets |
| title_sort | effects of dietary supplementation with lactobacillus acidophilus on the performance intestinal physical barrier function and the expression of nod like receptors in weaned piglets |
| topic | Lactobacillus acidophilus Intestinal physical barrier Weaned piglet NLRs |
| url | https://peerj.com/articles/6060.pdf |
| work_keys_str_mv | AT shiqiongwang effectsofdietarysupplementationwithlactobacillusacidophilusontheperformanceintestinalphysicalbarrierfunctionandtheexpressionofnodlikereceptorsinweanedpiglets AT haihuali effectsofdietarysupplementationwithlactobacillusacidophilusontheperformanceintestinalphysicalbarrierfunctionandtheexpressionofnodlikereceptorsinweanedpiglets AT chenhongdu effectsofdietarysupplementationwithlactobacillusacidophilusontheperformanceintestinalphysicalbarrierfunctionandtheexpressionofnodlikereceptorsinweanedpiglets AT qianliu effectsofdietarysupplementationwithlactobacillusacidophilusontheperformanceintestinalphysicalbarrierfunctionandtheexpressionofnodlikereceptorsinweanedpiglets AT dongjiyang effectsofdietarysupplementationwithlactobacillusacidophilusontheperformanceintestinalphysicalbarrierfunctionandtheexpressionofnodlikereceptorsinweanedpiglets AT longbinchen effectsofdietarysupplementationwithlactobacillusacidophilusontheperformanceintestinalphysicalbarrierfunctionandtheexpressionofnodlikereceptorsinweanedpiglets AT qizhu effectsofdietarysupplementationwithlactobacillusacidophilusontheperformanceintestinalphysicalbarrierfunctionandtheexpressionofnodlikereceptorsinweanedpiglets AT zhixiangwang effectsofdietarysupplementationwithlactobacillusacidophilusontheperformanceintestinalphysicalbarrierfunctionandtheexpressionofnodlikereceptorsinweanedpiglets |