The Evaluation Effect of methanol extracts from Prangos ferulacea and Prangos acaulis on Human lymphocytes proliferation and their mutagenicity in Ames test
Abstract Background: Medicinal plants are primery source of many drugs to cure different diseases. The genus Prangos, (Umbelliferae family) consists of several medicinal plants that their desirable dffects have been approved. The aim of this study is to investigate the effectiveness of methanol ext...
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| Format: | Article |
| Language: | fas |
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Arak Medical University
2015-07-01
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| Series: | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
| Subjects: | |
| Online Access: | http://amuj.arakmu.ac.ir/article-1-3405-en.pdf |
| _version_ | 1828493229968850944 |
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| author | Mokhtar Nosrati Mandana Behbahani |
| author_facet | Mokhtar Nosrati Mandana Behbahani |
| author_sort | Mokhtar Nosrati |
| collection | DOAJ |
| description | Abstract
Background: Medicinal plants are primery source of many drugs to cure different diseases. The genus Prangos, (Umbelliferae family) consists of several medicinal plants that their desirable dffects have been approved. The aim of this study is to investigate the effectiveness of methanol extract in different parts of prangos ferulacea and prangos acaualis on human lymphocytes proliferation and their mutagenicity in salmonella typhimurium TA98.
Materials and Methods: In this experimental study, the plants were collected from different areas of Kurdistan. Then, samples were air dried and powdered and methanol material of plants was extracted. The extracts were diluted to give concentrations of 10, 100, 500, 1000, 1500, 2000, 2500 μg/ml. Finally, the effects of these extracts on human lymphocytes proliferation and their mutagenecity have been investigated by the MTT and Ames test.
Results: The results showed that different organs extract from both tested plants caused a significant increase in lymphocytes proliferation, specially in concentrations of 500 to 2500 μg/ml. Of studied excrtacts, the highest and lowest effect on lymphocytes proliferation was obtained in presence of flower and seed, respectively. In total, the levels of proliferation resulted of prangos ferulacea as compared with prangos acaulis were higher. Also, the results of study showed no mutagenicity of studied plant exctracts with considered concentrations.
Conclusion: The findings revealed that both species of prangos can increase immune system function and were used as an safe medicinal plant to cure patients with immune deficiencies and microbial infections. |
| first_indexed | 2024-12-11T11:35:15Z |
| format | Article |
| id | doaj.art-7cdebb075da6434a8bd19e3ceb666b62 |
| institution | Directory Open Access Journal |
| issn | 1735-5338 2008-644X |
| language | fas |
| last_indexed | 2024-12-11T11:35:15Z |
| publishDate | 2015-07-01 |
| publisher | Arak Medical University |
| record_format | Article |
| series | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
| spelling | doaj.art-7cdebb075da6434a8bd19e3ceb666b622022-12-22T01:08:46ZfasArak Medical UniversityMajallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk1735-53382008-644X2015-07-011848193The Evaluation Effect of methanol extracts from Prangos ferulacea and Prangos acaulis on Human lymphocytes proliferation and their mutagenicity in Ames testMokhtar Nosrati0Mandana Behbahani1Department of Microbial Biotechnology, Isfahan University, Isfahan, IranDepartment of Microbial Biotechnology, Isfahan University, Isfahan, IranAbstract Background: Medicinal plants are primery source of many drugs to cure different diseases. The genus Prangos, (Umbelliferae family) consists of several medicinal plants that their desirable dffects have been approved. The aim of this study is to investigate the effectiveness of methanol extract in different parts of prangos ferulacea and prangos acaualis on human lymphocytes proliferation and their mutagenicity in salmonella typhimurium TA98. Materials and Methods: In this experimental study, the plants were collected from different areas of Kurdistan. Then, samples were air dried and powdered and methanol material of plants was extracted. The extracts were diluted to give concentrations of 10, 100, 500, 1000, 1500, 2000, 2500 μg/ml. Finally, the effects of these extracts on human lymphocytes proliferation and their mutagenecity have been investigated by the MTT and Ames test. Results: The results showed that different organs extract from both tested plants caused a significant increase in lymphocytes proliferation, specially in concentrations of 500 to 2500 μg/ml. Of studied excrtacts, the highest and lowest effect on lymphocytes proliferation was obtained in presence of flower and seed, respectively. In total, the levels of proliferation resulted of prangos ferulacea as compared with prangos acaulis were higher. Also, the results of study showed no mutagenicity of studied plant exctracts with considered concentrations. Conclusion: The findings revealed that both species of prangos can increase immune system function and were used as an safe medicinal plant to cure patients with immune deficiencies and microbial infections.http://amuj.arakmu.ac.ir/article-1-3405-en.pdflymphocytes proliferationMutagenesisPrangosSalmonella Typhimurium TA98 |
| spellingShingle | Mokhtar Nosrati Mandana Behbahani The Evaluation Effect of methanol extracts from Prangos ferulacea and Prangos acaulis on Human lymphocytes proliferation and their mutagenicity in Ames test Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk lymphocytes proliferation Mutagenesis Prangos Salmonella Typhimurium TA98 |
| title | The Evaluation Effect of methanol extracts from Prangos ferulacea and Prangos acaulis on Human lymphocytes proliferation and their mutagenicity in Ames test |
| title_full | The Evaluation Effect of methanol extracts from Prangos ferulacea and Prangos acaulis on Human lymphocytes proliferation and their mutagenicity in Ames test |
| title_fullStr | The Evaluation Effect of methanol extracts from Prangos ferulacea and Prangos acaulis on Human lymphocytes proliferation and their mutagenicity in Ames test |
| title_full_unstemmed | The Evaluation Effect of methanol extracts from Prangos ferulacea and Prangos acaulis on Human lymphocytes proliferation and their mutagenicity in Ames test |
| title_short | The Evaluation Effect of methanol extracts from Prangos ferulacea and Prangos acaulis on Human lymphocytes proliferation and their mutagenicity in Ames test |
| title_sort | evaluation effect of methanol extracts from prangos ferulacea and prangos acaulis on human lymphocytes proliferation and their mutagenicity in ames test |
| topic | lymphocytes proliferation Mutagenesis Prangos Salmonella Typhimurium TA98 |
| url | http://amuj.arakmu.ac.ir/article-1-3405-en.pdf |
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