Performance of Nano-Hydroxyapatite/Beta-Tricalcium Phosphate and Xenogenic Hydroxyapatite on Bone Regeneration in Rat Calvarial Defects: Histomorphometric, Immunohistochemical and Ultrastructural Analysis
Igor da Silva Brum,1 Lucio Frigo,2 Paulo Goncalo Pinto dos Santos,1 Carlos Nelson Elias,3 Guilherme Aparecido Monteiro Duque da Fonseca,2 Jorge Jose de Carvalho4 1Implantology Department, School of Dentistry, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil; 2Periodontology Departmen...
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| Format: | Article |
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Dove Medical Press
2021-05-01
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| Series: | International Journal of Nanomedicine |
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| Online Access: | https://www.dovepress.com/performance-of-nano-hydroxyapatitebeta-tricalcium-phosphate-and-xenoge-peer-reviewed-fulltext-article-IJN |
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| author | da Silva Brum I Frigo L Goncalo Pinto dos Santos P Nelson Elias C Fonseca GAMD Jose de Carvalho J |
| author_facet | da Silva Brum I Frigo L Goncalo Pinto dos Santos P Nelson Elias C Fonseca GAMD Jose de Carvalho J |
| author_sort | da Silva Brum I |
| collection | DOAJ |
| description | Igor da Silva Brum,1 Lucio Frigo,2 Paulo Goncalo Pinto dos Santos,1 Carlos Nelson Elias,3 Guilherme Aparecido Monteiro Duque da Fonseca,2 Jorge Jose de Carvalho4 1Implantology Department, School of Dentistry, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil; 2Periodontology Department, School of Dentistry, Universidade Guarulhos, São Paulo, Brazil; 3Instituto Militar de Engenharia, Rio de Janeiro, Brazil; 4Biology Department, School of Medicine, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, BrazilCorrespondence: Lucio FrigoPeriodontology Department, School of Dentistry, Universidade Guarulhos, Praça Teresa Cristina, 01, Guarulhos, São Paulo, 07023-070, BrazilTel/Fax +55-11- 2464-1684Email luciofrigo@uol.com.brBackground: Synthetic biomaterials have played an increasingly prominent role in the substitution of naturally derived biomaterials in current surgery practice. In vitro and in vivo characterization studies of new synthetic biomaterials are essential to analyze their physicochemical properties and the underlying mechanisms associated with the modulation of the inflammatory process and bone healing.Purpose: This study compares the in vivo tissue behavior of a synthetic biomaterial nano-hydroxyapatite/beta-tricalcium phosphate (nano-HA/ß-TCP mixture) and deproteinized bovine bone mineral (DBBM) in a rat calvarial defect model. The innovation of this work is in the comparative analysis of the effect of new synthetic and commercially xenogenic biomaterials on the inflammatory response, bone matrix gain, and stimulation of osteoclastogenesis and osteoblastogenesis.Methods: Both biomaterials were inserted in rat defects. The animals were divided into three groups, in which calvarial defects were filled with xenogenic biomaterials (group 1) and synthetic biomaterials (group 2), or left unfilled (group 3, controls). Sixty days after calvarial bone defects filled with biomaterials, periodic acid Schiff (PAS) and Masson’s trichrome staining, immunohistochemistry tumor necrosis factor-alpha (TNF-α), matrix metalloproteinase-9 (MMP-9), and electron microscopy analyses were conducted.Results: Histomorphometric analysis revealed powerful effects such as a higher amount of proteinaceous matrix and higher levels of TNF-α and MMP-9 in bone defects treated with alloplastic nano-HA/ß-TCP mixture than xenogenicxenogic biomaterial, as well as collagen-proteinaceous material in association with hydroxyapatite crystalloids.Conclusion: These data indicate that the synthetic nano-HA/ß-TCP mixture enhanced bone formation/remodeling in rat calvarial bone defects. The nano-HA/ß-TCP did not present risks of cross-infection/disease transmission. The synthetic nano-hydroxyapatite/beta-tricalcium phosphate mixture presented adequate properties for guided bone regeneration and guided tissue regeneration for dental surgical procedures.Keywords: biomaterial, hydroxyapatite, beta-tricalcium phosphate, bone healing, histomorphometry |
| first_indexed | 2024-12-19T02:41:20Z |
| format | Article |
| id | doaj.art-7ce1994306be49a5bc26f8f7e328a6c5 |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-19T02:41:20Z |
| publishDate | 2021-05-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-7ce1994306be49a5bc26f8f7e328a6c52022-12-21T20:39:07ZengDove Medical PressInternational Journal of Nanomedicine1178-20132021-05-01Volume 163473348564862Performance of Nano-Hydroxyapatite/Beta-Tricalcium Phosphate and Xenogenic Hydroxyapatite on Bone Regeneration in Rat Calvarial Defects: Histomorphometric, Immunohistochemical and Ultrastructural Analysisda Silva Brum IFrigo LGoncalo Pinto dos Santos PNelson Elias CFonseca GAMDJose de Carvalho JIgor da Silva Brum,1 Lucio Frigo,2 Paulo Goncalo Pinto dos Santos,1 Carlos Nelson Elias,3 Guilherme Aparecido Monteiro Duque da Fonseca,2 Jorge Jose de Carvalho4 1Implantology Department, School of Dentistry, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil; 2Periodontology Department, School of Dentistry, Universidade Guarulhos, São Paulo, Brazil; 3Instituto Militar de Engenharia, Rio de Janeiro, Brazil; 4Biology Department, School of Medicine, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, BrazilCorrespondence: Lucio FrigoPeriodontology Department, School of Dentistry, Universidade Guarulhos, Praça Teresa Cristina, 01, Guarulhos, São Paulo, 07023-070, BrazilTel/Fax +55-11- 2464-1684Email luciofrigo@uol.com.brBackground: Synthetic biomaterials have played an increasingly prominent role in the substitution of naturally derived biomaterials in current surgery practice. In vitro and in vivo characterization studies of new synthetic biomaterials are essential to analyze their physicochemical properties and the underlying mechanisms associated with the modulation of the inflammatory process and bone healing.Purpose: This study compares the in vivo tissue behavior of a synthetic biomaterial nano-hydroxyapatite/beta-tricalcium phosphate (nano-HA/ß-TCP mixture) and deproteinized bovine bone mineral (DBBM) in a rat calvarial defect model. The innovation of this work is in the comparative analysis of the effect of new synthetic and commercially xenogenic biomaterials on the inflammatory response, bone matrix gain, and stimulation of osteoclastogenesis and osteoblastogenesis.Methods: Both biomaterials were inserted in rat defects. The animals were divided into three groups, in which calvarial defects were filled with xenogenic biomaterials (group 1) and synthetic biomaterials (group 2), or left unfilled (group 3, controls). Sixty days after calvarial bone defects filled with biomaterials, periodic acid Schiff (PAS) and Masson’s trichrome staining, immunohistochemistry tumor necrosis factor-alpha (TNF-α), matrix metalloproteinase-9 (MMP-9), and electron microscopy analyses were conducted.Results: Histomorphometric analysis revealed powerful effects such as a higher amount of proteinaceous matrix and higher levels of TNF-α and MMP-9 in bone defects treated with alloplastic nano-HA/ß-TCP mixture than xenogenicxenogic biomaterial, as well as collagen-proteinaceous material in association with hydroxyapatite crystalloids.Conclusion: These data indicate that the synthetic nano-HA/ß-TCP mixture enhanced bone formation/remodeling in rat calvarial bone defects. The nano-HA/ß-TCP did not present risks of cross-infection/disease transmission. The synthetic nano-hydroxyapatite/beta-tricalcium phosphate mixture presented adequate properties for guided bone regeneration and guided tissue regeneration for dental surgical procedures.Keywords: biomaterial, hydroxyapatite, beta-tricalcium phosphate, bone healing, histomorphometryhttps://www.dovepress.com/performance-of-nano-hydroxyapatitebeta-tricalcium-phosphate-and-xenoge-peer-reviewed-fulltext-article-IJNbiomaterialhydroxyapatitebeta-tricalcium phosphatebone healinghistomorphometry |
| spellingShingle | da Silva Brum I Frigo L Goncalo Pinto dos Santos P Nelson Elias C Fonseca GAMD Jose de Carvalho J Performance of Nano-Hydroxyapatite/Beta-Tricalcium Phosphate and Xenogenic Hydroxyapatite on Bone Regeneration in Rat Calvarial Defects: Histomorphometric, Immunohistochemical and Ultrastructural Analysis International Journal of Nanomedicine biomaterial hydroxyapatite beta-tricalcium phosphate bone healing histomorphometry |
| title | Performance of Nano-Hydroxyapatite/Beta-Tricalcium Phosphate and Xenogenic Hydroxyapatite on Bone Regeneration in Rat Calvarial Defects: Histomorphometric, Immunohistochemical and Ultrastructural Analysis |
| title_full | Performance of Nano-Hydroxyapatite/Beta-Tricalcium Phosphate and Xenogenic Hydroxyapatite on Bone Regeneration in Rat Calvarial Defects: Histomorphometric, Immunohistochemical and Ultrastructural Analysis |
| title_fullStr | Performance of Nano-Hydroxyapatite/Beta-Tricalcium Phosphate and Xenogenic Hydroxyapatite on Bone Regeneration in Rat Calvarial Defects: Histomorphometric, Immunohistochemical and Ultrastructural Analysis |
| title_full_unstemmed | Performance of Nano-Hydroxyapatite/Beta-Tricalcium Phosphate and Xenogenic Hydroxyapatite on Bone Regeneration in Rat Calvarial Defects: Histomorphometric, Immunohistochemical and Ultrastructural Analysis |
| title_short | Performance of Nano-Hydroxyapatite/Beta-Tricalcium Phosphate and Xenogenic Hydroxyapatite on Bone Regeneration in Rat Calvarial Defects: Histomorphometric, Immunohistochemical and Ultrastructural Analysis |
| title_sort | performance of nano hydroxyapatite beta tricalcium phosphate and xenogenic hydroxyapatite on bone regeneration in rat calvarial defects histomorphometric immunohistochemical and ultrastructural analysis |
| topic | biomaterial hydroxyapatite beta-tricalcium phosphate bone healing histomorphometry |
| url | https://www.dovepress.com/performance-of-nano-hydroxyapatitebeta-tricalcium-phosphate-and-xenoge-peer-reviewed-fulltext-article-IJN |
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