Isolation, Identification and Antimicrobial Susceptibility of Brucella Isolates From Human Cases
Introduction: Brucellosis is a worldwide zoonotic disease that remains an important public health problem in India. As clinical manifestations of human brucellosis are variable in nature, and no constellation of clinical findings can be considered, characteristic laboratory help is must in the d...
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JCDR Research and Publications Pvt. Ltd.
2015-10-01
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| author | Smita S Mangalgi Archana Prakash Selvam Duraipandian Thava Annapurna G. Sajjan Shivajirao T. Mohite Shivali Gajul |
| author_facet | Smita S Mangalgi Archana Prakash Selvam Duraipandian Thava Annapurna G. Sajjan Shivajirao T. Mohite Shivali Gajul |
| author_sort | Smita S Mangalgi |
| collection | DOAJ |
| description | Introduction: Brucellosis is a worldwide zoonotic disease that
remains an important public health problem in India. As clinical
manifestations of human brucellosis are variable in nature,
and no constellation of clinical findings can be considered,
characteristic laboratory help is must in the diagnosis. In the
laboratory, brucellosis is generally diagnosed by serological
tests. Though many serological tests with different principles
are available, serological testing does not provide direct
evidence for the presence of the pathogen, hence isolation of
Brucella spp. from the clinical specimen is considered to be
the gold standard. Brucella is highly infectious and requires
level 3 bio-containment facilities and technically skilled
personnel, Brucella cultures are rarely performed. Due to the
rampant use of rifampin for the treatment of tuberculosis and
reports on development of resistance in virtually all organisms
the sensitivity of Brucellae to the traditional drugs cannot be
presumed.
Aim: To isolate, identify the Brucellae from blood culture and
to assess the antimicrobial susceptibility of the isolates to
WHO recommended anti-brucellar antibiotics by agar dilution
method.
Materials and Methods: A total of 169 blood samples were
collected for brucella culture, from 593 epidemiologically,
clinically and serologically suspected cases of human
brucellosis. Of the 169 blood cultures 77 yielded Brucella,
of which nine were from the western Maharashtra and 68
from Northern Karnataka. The isolates were identified using
conventional methods. The minimal inhibitory concentration
(MIC) values for streptomycin, gentamicin, doxycycline,
rifampin and trimethoprim-sulfamethoxazole were determined
by using agar dilution method.
Results: Isolation rate for Brucella was 45.5%. Of the 77 isolates,
75 were identified as B. melitensis and two as B. abortus. All the
isolates were sensitive to all the drugs tested.
Conclusion: Human brucellosis due to B.melitensis is fairly a
common disease in this area. The current WHO recommended
drug regimen for the treatment of human brucellosis continues
to be effective as no drug resistance is noted in the study |
| first_indexed | 2024-12-22T02:04:17Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2277-8551 2455-6882 |
| language | English |
| last_indexed | 2024-12-22T02:04:17Z |
| publishDate | 2015-10-01 |
| publisher | JCDR Research and Publications Pvt. Ltd. |
| record_format | Article |
| series | National Journal of Laboratory Medicine |
| spelling | doaj.art-7ceb6ca9064a44fcbeb01585f35373ae2022-12-21T18:42:33ZengJCDR Research and Publications Pvt. Ltd.National Journal of Laboratory Medicine2277-85512455-68822015-10-0144394210.7860/NJLM/2015/14156:2070Isolation, Identification and Antimicrobial Susceptibility of Brucella Isolates From Human CasesSmita S Mangalgi0Archana Prakash1Selvam Duraipandian Thava2Annapurna G. Sajjan3Shivajirao T. Mohite4Shivali Gajul5Assistant Professor, Department of Microbiology, BLDEU’s Shri B M Patil Medical College, Bijapur, Karnataka, India.Research Scholar, MB Division, D.R.D.E. Gwalior, India.Scientist E, and Deputy Director, Microbiology Division D.R.D.E. Gwalior, India.. Professor, Department of Microbiology, BLDEU’s Shri B M Patil Medical College, Bijapur, Karnataka, India.Professor, Department of Microbiology, Krishna Institute of Medical Sciences, Karad – Maharashtra, India.Assistant Professor, Department of Microbiology, BLDEU’s Shri B M Patil Medical College, Bijapur Karnataka, India.Introduction: Brucellosis is a worldwide zoonotic disease that remains an important public health problem in India. As clinical manifestations of human brucellosis are variable in nature, and no constellation of clinical findings can be considered, characteristic laboratory help is must in the diagnosis. In the laboratory, brucellosis is generally diagnosed by serological tests. Though many serological tests with different principles are available, serological testing does not provide direct evidence for the presence of the pathogen, hence isolation of Brucella spp. from the clinical specimen is considered to be the gold standard. Brucella is highly infectious and requires level 3 bio-containment facilities and technically skilled personnel, Brucella cultures are rarely performed. Due to the rampant use of rifampin for the treatment of tuberculosis and reports on development of resistance in virtually all organisms the sensitivity of Brucellae to the traditional drugs cannot be presumed. Aim: To isolate, identify the Brucellae from blood culture and to assess the antimicrobial susceptibility of the isolates to WHO recommended anti-brucellar antibiotics by agar dilution method. Materials and Methods: A total of 169 blood samples were collected for brucella culture, from 593 epidemiologically, clinically and serologically suspected cases of human brucellosis. Of the 169 blood cultures 77 yielded Brucella, of which nine were from the western Maharashtra and 68 from Northern Karnataka. The isolates were identified using conventional methods. The minimal inhibitory concentration (MIC) values for streptomycin, gentamicin, doxycycline, rifampin and trimethoprim-sulfamethoxazole were determined by using agar dilution method. Results: Isolation rate for Brucella was 45.5%. Of the 77 isolates, 75 were identified as B. melitensis and two as B. abortus. All the isolates were sensitive to all the drugs tested. Conclusion: Human brucellosis due to B.melitensis is fairly a common disease in this area. The current WHO recommended drug regimen for the treatment of human brucellosis continues to be effective as no drug resistance is noted in the studyhttp://www.njlm.net/articles/PDF/2070/9-%2014156_F(AK)_PF1(VSUAK)_PFA(AK)_PF2(PVSU).pdfagar dilution methodantibiotic sensitivityblood culture |
| spellingShingle | Smita S Mangalgi Archana Prakash Selvam Duraipandian Thava Annapurna G. Sajjan Shivajirao T. Mohite Shivali Gajul Isolation, Identification and Antimicrobial Susceptibility of Brucella Isolates From Human Cases National Journal of Laboratory Medicine agar dilution method antibiotic sensitivity blood culture |
| title | Isolation, Identification and Antimicrobial Susceptibility of Brucella Isolates From Human Cases |
| title_full | Isolation, Identification and Antimicrobial Susceptibility of Brucella Isolates From Human Cases |
| title_fullStr | Isolation, Identification and Antimicrobial Susceptibility of Brucella Isolates From Human Cases |
| title_full_unstemmed | Isolation, Identification and Antimicrobial Susceptibility of Brucella Isolates From Human Cases |
| title_short | Isolation, Identification and Antimicrobial Susceptibility of Brucella Isolates From Human Cases |
| title_sort | isolation identification and antimicrobial susceptibility of brucella isolates from human cases |
| topic | agar dilution method antibiotic sensitivity blood culture |
| url | http://www.njlm.net/articles/PDF/2070/9-%2014156_F(AK)_PF1(VSUAK)_PFA(AK)_PF2(PVSU).pdf |
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