Evaluation of NS1 Antigen Detection for Early Diagnosis of Dengue in a Tertiary Hospital in Southern India
Introduction: Dengue is a mosquito-borne disease affecting mainly tropical and subtropical regions of the world. The early diagnosis of dengue is required for identifying an epidemic and also for implementing effective vector control measures. Aim: To evaluate NS1 antigen assay as an alternative...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2016-04-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/7562/15758_CE[Ra1]_F(AK)_PF1(BMAK)_PFA(AK)_PF2(PAG).pdf |
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| author | Ashwini Manoor Anand Sujatha Sistla Rahul Dhodapkar Abdoul Hamide Niranjan Biswal Badrinath Srinivasan |
| author_facet | Ashwini Manoor Anand Sujatha Sistla Rahul Dhodapkar Abdoul Hamide Niranjan Biswal Badrinath Srinivasan |
| author_sort | Ashwini Manoor Anand |
| collection | DOAJ |
| description | Introduction: Dengue is a mosquito-borne disease affecting
mainly tropical and subtropical regions of the world. The early
diagnosis of dengue is required for identifying an epidemic and
also for implementing effective vector control measures.
Aim: To evaluate NS1 antigen assay as an alternative to RTPCR for the early diagnosis of Dengue.
Materials and Methods: A comparative study was conducted
to evaluate NS1 antigen assay in clinically suspected dengue
cases admitted to JIPMER hospital from January to November
2011. Serum samples were tested for NS1 antigen, IgM and IgG
antibodies by ELISA and RT-PCR.
Results: Out of total 112 clinically suspected dengue, 94 were
laboratory-confirmed dengue cases (positive by one or more
of the following tests - IgM ELISA, NS1 antigen ELISA and RTPCR). NS1 was detectable from day 1 to day 12 of fever. The
positive detection rate of NS1 antigen ELISA, RT-PCR and IgM
ELISA were 80.9%, 68.1% and 47.9% respectively. NS1 antigen
ELISA was evaluated using RT-PCR as the reference standard
and showed a sensitivity of 96.8%, specificity of 53.3%,
positive predictive value of 81.6% and negative predictive value
of 88.9% with a likelihood ratio of 2.1 by Fisher’s-exact test.
The combination of NS1 and IgM had the highest sensitivity
of 97.8%. DEN-3 was the serotype identified by RT-PCR for
24 randomly selected samples. NS1 antigen detection had the
highest sensitivity in the early stages while IgM detection was
more sensitive in the later half of the illness.
Conclusion: Both NS1 and RT-PCR are useful for early dengue
diagnosis, although in terms of cost, ease of performance
and rapidity, NS1 is superior to RT-PCR. NS1 in combination
with IgM assay offers the most sensitive and cost-effective
diagnostic modality for dengue. |
| first_indexed | 2024-12-23T10:08:12Z |
| format | Article |
| id | doaj.art-7cecf9fb7b31402c814efd1ef6e348c0 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-23T10:08:12Z |
| publishDate | 2016-04-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-7cecf9fb7b31402c814efd1ef6e348c02022-12-21T17:51:02ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2016-04-01104DC01DC0410.7860/JCDR/2016/15758.7562Evaluation of NS1 Antigen Detection for Early Diagnosis of Dengue in a Tertiary Hospital in Southern IndiaAshwini Manoor Anand0Sujatha Sistla1Rahul Dhodapkar2Abdoul Hamide3Niranjan Biswal4Badrinath Srinivasan5Senior Resident, Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education & Research (JIPMER), Puducherry, India.Professor, Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education & Research (JIPMER), Puducherry, India.Associate Professor, Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education & Research (JIPMER), Puducherry, India.Professor, Department of Medicine, Jawaharlal Institute of Postgraduate Medical Education & Research (JIPMER), Puducherry, India.Professor, Department of Medicine, Jawaharlal Institute of Postgraduate Medical Education & Research (JIPMER), Puducherry, India.Professor, Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education & Research (JIPMER), Puducherry, India.Introduction: Dengue is a mosquito-borne disease affecting mainly tropical and subtropical regions of the world. The early diagnosis of dengue is required for identifying an epidemic and also for implementing effective vector control measures. Aim: To evaluate NS1 antigen assay as an alternative to RTPCR for the early diagnosis of Dengue. Materials and Methods: A comparative study was conducted to evaluate NS1 antigen assay in clinically suspected dengue cases admitted to JIPMER hospital from January to November 2011. Serum samples were tested for NS1 antigen, IgM and IgG antibodies by ELISA and RT-PCR. Results: Out of total 112 clinically suspected dengue, 94 were laboratory-confirmed dengue cases (positive by one or more of the following tests - IgM ELISA, NS1 antigen ELISA and RTPCR). NS1 was detectable from day 1 to day 12 of fever. The positive detection rate of NS1 antigen ELISA, RT-PCR and IgM ELISA were 80.9%, 68.1% and 47.9% respectively. NS1 antigen ELISA was evaluated using RT-PCR as the reference standard and showed a sensitivity of 96.8%, specificity of 53.3%, positive predictive value of 81.6% and negative predictive value of 88.9% with a likelihood ratio of 2.1 by Fisher’s-exact test. The combination of NS1 and IgM had the highest sensitivity of 97.8%. DEN-3 was the serotype identified by RT-PCR for 24 randomly selected samples. NS1 antigen detection had the highest sensitivity in the early stages while IgM detection was more sensitive in the later half of the illness. Conclusion: Both NS1 and RT-PCR are useful for early dengue diagnosis, although in terms of cost, ease of performance and rapidity, NS1 is superior to RT-PCR. NS1 in combination with IgM assay offers the most sensitive and cost-effective diagnostic modality for dengue.https://jcdr.net/articles/PDF/7562/15758_CE[Ra1]_F(AK)_PF1(BMAK)_PFA(AK)_PF2(PAG).pdfdengue diagnosiscomparisonns1 antigen assaydengue rt-pcrdengue igm elisa |
| spellingShingle | Ashwini Manoor Anand Sujatha Sistla Rahul Dhodapkar Abdoul Hamide Niranjan Biswal Badrinath Srinivasan Evaluation of NS1 Antigen Detection for Early Diagnosis of Dengue in a Tertiary Hospital in Southern India Journal of Clinical and Diagnostic Research dengue diagnosis comparison ns1 antigen assay dengue rt-pcr dengue igm elisa |
| title | Evaluation of NS1 Antigen Detection for Early Diagnosis of Dengue in a Tertiary Hospital in Southern India |
| title_full | Evaluation of NS1 Antigen Detection for Early Diagnosis of Dengue in a Tertiary Hospital in Southern India |
| title_fullStr | Evaluation of NS1 Antigen Detection for Early Diagnosis of Dengue in a Tertiary Hospital in Southern India |
| title_full_unstemmed | Evaluation of NS1 Antigen Detection for Early Diagnosis of Dengue in a Tertiary Hospital in Southern India |
| title_short | Evaluation of NS1 Antigen Detection for Early Diagnosis of Dengue in a Tertiary Hospital in Southern India |
| title_sort | evaluation of ns1 antigen detection for early diagnosis of dengue in a tertiary hospital in southern india |
| topic | dengue diagnosis comparison ns1 antigen assay dengue rt-pcr dengue igm elisa |
| url | https://jcdr.net/articles/PDF/7562/15758_CE[Ra1]_F(AK)_PF1(BMAK)_PFA(AK)_PF2(PAG).pdf |
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