Manganese Mineralization of Pathogenic Viruses as a Universal Vaccine Platform
Abstract Biomimetic viral mineralization improves viral vaccine stability and immunogenicity using inorganic metals such as Ca, Al, or Fe. Mn is a metal found in high concentrations in mammalian tissues; however, under natural or laboratory conditions, Mn mineralization by medical viruses has yet to...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-11-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202303615 |
| _version_ | 1797465034896441344 |
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| author | Pan‐Deng Shi Yan‐Peng Xu Zhu Zhu Chao Zhou Mei Wu Yangzhige He Hui Zhao Liying Liu Linqing Zhao Xiao‐Feng Li Cheng‐Feng Qin |
| author_facet | Pan‐Deng Shi Yan‐Peng Xu Zhu Zhu Chao Zhou Mei Wu Yangzhige He Hui Zhao Liying Liu Linqing Zhao Xiao‐Feng Li Cheng‐Feng Qin |
| author_sort | Pan‐Deng Shi |
| collection | DOAJ |
| description | Abstract Biomimetic viral mineralization improves viral vaccine stability and immunogenicity using inorganic metals such as Ca, Al, or Fe. Mn is a metal found in high concentrations in mammalian tissues; however, under natural or laboratory conditions, Mn mineralization by medical viruses has yet to be established. Herein, a single IAV particle is successfully encapsulated with manganese phosphate (MnP) under specific conditions using the human influenza A virus (IAV). MnP‐mineralized IAVs (IAV@Mn) exhibited physiochemical and in vitro properties similar to Ca‐mineralized IAVs. In animal models, IAV@Mn shows limited replication in immune‐competent cells and a significant attenuation compared to naïve cells. Moreover, a single‐dose vaccination with IAV@Mn induced robust humoral and cellular immune responses and conferred significant protection against a wild‐type IAV challenge in mice. Thus, Mn mineralization in pathogenic viruses provides a rapid and universal strategy for generating an emergency vaccine in response to emerging viruses. |
| first_indexed | 2024-03-09T18:16:43Z |
| format | Article |
| id | doaj.art-7cf08426f3cb491d85001128fe49b83e |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-03-09T18:16:43Z |
| publishDate | 2023-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-7cf08426f3cb491d85001128fe49b83e2023-11-24T08:40:29ZengWileyAdvanced Science2198-38442023-11-011033n/an/a10.1002/advs.202303615Manganese Mineralization of Pathogenic Viruses as a Universal Vaccine PlatformPan‐Deng Shi0Yan‐Peng Xu1Zhu Zhu2Chao Zhou3Mei Wu4Yangzhige He5Hui Zhao6Liying Liu7Linqing Zhao8Xiao‐Feng Li9Cheng‐Feng Qin10State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Academy of Military Medical Sciences Beijing 100071 ChinaLaboratory of Virology Beijing Key Laboratory of Etiology of Viral Diseases in Children Capital Institute of Pediatrics Beijing 100020 ChinaState Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Academy of Military Medical Sciences Beijing 100071 ChinaState Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Academy of Military Medical Sciences Beijing 100071 ChinaState Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Academy of Military Medical Sciences Beijing 100071 ChinaDepartment of Medical Research Center State Key Laboratory of Complex Severe and Rare Diseases Peking Union Medical College Hospital Chinese Academy of Medical Science & Peking Union Medical College Beijing 100730 ChinaState Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Academy of Military Medical Sciences Beijing 100071 ChinaLaboratory of Virology Beijing Key Laboratory of Etiology of Viral Diseases in Children Capital Institute of Pediatrics Beijing 100020 ChinaLaboratory of Virology Beijing Key Laboratory of Etiology of Viral Diseases in Children Capital Institute of Pediatrics Beijing 100020 ChinaState Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Academy of Military Medical Sciences Beijing 100071 ChinaState Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Academy of Military Medical Sciences Beijing 100071 ChinaAbstract Biomimetic viral mineralization improves viral vaccine stability and immunogenicity using inorganic metals such as Ca, Al, or Fe. Mn is a metal found in high concentrations in mammalian tissues; however, under natural or laboratory conditions, Mn mineralization by medical viruses has yet to be established. Herein, a single IAV particle is successfully encapsulated with manganese phosphate (MnP) under specific conditions using the human influenza A virus (IAV). MnP‐mineralized IAVs (IAV@Mn) exhibited physiochemical and in vitro properties similar to Ca‐mineralized IAVs. In animal models, IAV@Mn shows limited replication in immune‐competent cells and a significant attenuation compared to naïve cells. Moreover, a single‐dose vaccination with IAV@Mn induced robust humoral and cellular immune responses and conferred significant protection against a wild‐type IAV challenge in mice. Thus, Mn mineralization in pathogenic viruses provides a rapid and universal strategy for generating an emergency vaccine in response to emerging viruses.https://doi.org/10.1002/advs.202303615innate immune activationmanganese mineralizationnovel vaccineresist infectionvirulence attenuation |
| spellingShingle | Pan‐Deng Shi Yan‐Peng Xu Zhu Zhu Chao Zhou Mei Wu Yangzhige He Hui Zhao Liying Liu Linqing Zhao Xiao‐Feng Li Cheng‐Feng Qin Manganese Mineralization of Pathogenic Viruses as a Universal Vaccine Platform Advanced Science innate immune activation manganese mineralization novel vaccine resist infection virulence attenuation |
| title | Manganese Mineralization of Pathogenic Viruses as a Universal Vaccine Platform |
| title_full | Manganese Mineralization of Pathogenic Viruses as a Universal Vaccine Platform |
| title_fullStr | Manganese Mineralization of Pathogenic Viruses as a Universal Vaccine Platform |
| title_full_unstemmed | Manganese Mineralization of Pathogenic Viruses as a Universal Vaccine Platform |
| title_short | Manganese Mineralization of Pathogenic Viruses as a Universal Vaccine Platform |
| title_sort | manganese mineralization of pathogenic viruses as a universal vaccine platform |
| topic | innate immune activation manganese mineralization novel vaccine resist infection virulence attenuation |
| url | https://doi.org/10.1002/advs.202303615 |
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